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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS03725 [old locus tag: SACOL0725 ]
- pan locus tag?: SAUPAN002547000
- symbol: SACOL_RS03725
- pan gene symbol?: rbf
- synonym:
- product: AraC family transcriptional regulator
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS03725 [old locus tag: SACOL0725 ]
- symbol: SACOL_RS03725
- product: AraC family transcriptional regulator
- replicon: chromosome
- strand: +
- coordinates: 750431..752581
- length: 2151
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2101ATGGCAAAATCATGCTTGCATATACTTACTAATAATGAATATGCGACAACGCGTTGCCAA
GATGGCATAGTCTTATTTTGGCCAATTGACGGGGAAATCGAACTACAAAAATTTCGTAAA
AGTAAAATAATTGAAGATGATATATATATTATTAATCATCTGGATGTATTTAGTATTAAG
AATAATAAAAAAACGATCATGTTGTATTTGAGTAGCGATTGGTTTGCGGAATTAGGCTTT
ACTTTCTTTAATTACCACTATACAGCAAAGTTGATTAAATCATCCTATAATTTGAAATGT
CTACTATTAAAATTGACATATCGATACCTTGATAATCAGCCTCTTAATGACGCTGATATT
AGAAAATTACAGGATATTATTAAAATCATTGCAAAAGAAGCAAGTATGGATAAAAAGATT
GCACAAAATCAATATCGATATGCGTATTATGGTGATTTGCGTGATGAGCTCGAATATATT
TATCAAAATGTAAATCAACGATTGACATTAAAAAGTGTCGCTGATAAATTATTTGTCTCA
AAGTCAAATTTGTCATCACAATTCCACTTACTTATGGGCATGGGTTTTAAAAAATATATT
GATACTTTGAAAATTGGTAAATCGATTGAAATTCTACTTACTACTGATAGTACTATTAGC
AACATAAGTGAACATTTAGGTTTTAGTAGTAGCTCCACTTACTCTAAAATGTTTAAAAGT
TATATGGATATAACACCGAATGAATATCGTAATTTATCAAAATATAATAAATGTTTAATG
CTAAAGCCAGAACCACTAGTAGGCAAAATGGTGCAAGAAGTAAAAGAAATCATATTGAAT
TATATTGAACATTATAAAAACCACCTAACTGATGTTATACATATTGATGAAGACAAATTT
GAAACACCTAAATTGTTTCAAACGGTTATTCAAATAAATACTTATACAGAAATGAAATTA
GTTTTCTTAGAAGGAATCTTTAAAACCTTATTGAATAAGAACAGTCAAGTTGTCTTTTTC
ATCATGCCATCGATTCTAAAAAGTAAAAATACCATGTCCGAAGAAGAAAAATTCACAATC
ATTAAAACAATAATTGAAAGTGATCTAAAGATAGCATTTAATATAAATAATATTGAAACA
ACTTATTTTGTTGAAGAAGCTTTTATGAGTGTTTTCAGACAAATATCTCCAAACGAATTA
AGTAATCATAATAATTACGAAGTGCATTTTGTTTTTGATTTATCATTGATGGAAATTAGA
ACAATTTATCGAATGATATTAAAATTACATAACATCATGTTGAATGTGAAATTAGGATTG
AACATTACCTGTTTATTTGAAAAACCTTCAGTTTTTAAATCACTAGTATCACAAATAAAG
CGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATG
GGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTA
ATTAATGAATTGGATATTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAA
CTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTA
AGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAAACATTTTTGACAATCATCAAGCA
TTTAATGCGATGCATCTATATGACAAAAATGGATTTAAAACAACACTAGGTCTTATATTA
GAAAAGTTTATCGAATATGTCTCGAAACCAAAATACGAAAACAGTTATTATTCTATTTTT
GATATAGAGAATTATTATTGTCTTGTTATTTATGATTGGCGAGTGATAGAGAGCGAGACA
ATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTTAAAAACAATGTTTTAAAC
GATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTG
ATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGAC
AACTACCTTAAACCAGCAATAGAGATTAAAGAGCATAATTTTAGTGATAATTCCTTGAAT
ATTAACGTTACTTTTAATGCGTTATACATAATTAAAATAGGAAAAAAATAA60
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2151
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS03725 [old locus tag: SACOL0725 ]
- symbol: SACOL_RS03725
- description: AraC family transcriptional regulator
- length: 716
- theoretical pI: 7.02006
- theoretical MW: 84116.6
- GRAVY: -0.215922
⊟Function[edit | edit source]
- TIGRFAM: YSIRK-targeted surface antigen transcriptional regulator (TIGR04094; HMM-score: 62)and 1 more4-hydroxyphenylacetate catabolism regulatory protein HpaA (TIGR02297; HMM-score: 27.8)
- TheSEED: see SACOL0725
- PFAM: HTH (CL0123) HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 60.2)HTH_18; Helix-turn-helix domain (PF12833; HMM-score: 51.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.78
- Cytoplasmic Membrane Score: 8.16
- Cellwall Score: 0.06
- Extracellular Score: 0.01
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003011
- TAT(Tat/SPI): 0.000108
- LIPO(Sec/SPII): 0.000899
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKIIEDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLLLKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYIYQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDSTISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKEIILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKNSQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINNIETTYFVEEAFMSVFRQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFEKPSVFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFKNLKQVINELDIEQEKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLNIFDNHQAFNAMHLYDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIYDWRVIESETIMSNFEDSQVYINFKNNVLNDKYLIVIETLDENSGNINHLISKELRDKYEWNPSLLSKIDNYLKPAIEIKEHNFSDNSLNINVTFNALYIIKIGKK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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