From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03995 [old locus tag: SACOL0777 ]
  • pan locus tag?: SAUPAN002604000
  • symbol: SACOL_RS03995
  • pan gene symbol?:
  • synonym:
  • product: allophanate hydrolase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03995 [old locus tag: SACOL0777 ]
  • symbol: SACOL_RS03995
  • product: allophanate hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 797212..798216
  • length: 1005
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (797212..798216) NCBI
  • BioCyc: SACOL_RS03995 BioCyc
  • MicrobesOnline: see SACOL0777

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCAATCATAATTGAAAAAAGTGGCTTATTCAGTAGCTTTCAGGACTTTGGCAGAAGG
    GGATATGAACATGATGGTGTAATTCCATGTGGTGCACTTGATACTTTAGCACATGAAATT
    GCTAATCGATTAGTTGCAAATGACAAGAATGAAGCAACTTTGGAAATGACTAATAAAATG
    GCAACGATTCGTTTTACAGAACCTACGCTGATTGCATTAGCAGGGGGTAATGTCAAAGCT
    TACACTGAGCATATGACTATATCTCCATATAAATTGTATTTGTTAGATAAAGGCGATGTT
    TTAAAGTTTAGAGAAACAAGTTATACATCGCGAGTGTATTTAGCTGTGGGAGGCGGATTT
    GAATTAGATGCATGGTTAGGATCTAACTCAACCGACTTTAATGTAAAAATTGGTGGTTTT
    AAAGGTAGAACATTACAAGATGGCGATGAAATAAAGCTTAAGAGAGATTATACAGCTCGT
    CATCATAAGTTATTTGAAAACCTTGCTCACACGAAACAAACAGATTGGGGTATTGATGGA
    TACGCCTTGTCATTTAATTATATGTCTGATGTATTTCATGTCGTTAAAAATAAAGGTACG
    GAAGATTTTAAAGAAGATGCCATTCAAAGATTTGTGAAACATGATTATAAAGTAACGAGC
    AAAGCAAATCGCATGGGGATGATGCTTGAAGGTGAAAAAATCAAAGCTTTTTATGAAGAT
    ATGCCACCGTATCAGACTGTCAAAAAAGGAACGATACAAATTAAGCGTGATGGCACACCT
    ATTATCCTATTAAATGATCATTATACGCTAGGTAGCTACCCGCAAATCGGTACAATCGCA
    AGTTATCATTTAACGAAATTAGCACAAAAACCGCAAGGATCACGTTTGAAATTTCAATTT
    ATAGATATTTTAACGGCTGAAAAGAACCTTGTTAAGTATAGTAACTGGTTAAACCAATTA
    TTCCATGGAATAGAATATAGAATGCAATTAGAAATGATGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS03995 [old locus tag: SACOL0777 ]
  • symbol: SACOL_RS03995
  • description: allophanate hydrolase
  • length: 334
  • theoretical pI: 9.15837
  • theoretical MW: 38133.4
  • GRAVY: -0.428743

Function[edit | edit source]

  • TIGRFAM:
    Unknown function General biotin-dependent carboxylase uncharacterized domain (TIGR00724; HMM-score: 230.1)
    and 1 more
    Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 135.3)
  • TheSEED: see SACOL0777
  • PFAM:
    Cyclophil-like (CL0475) CT_A_B; Carboxyltransferase domain, subdomain A and B (PF02626; HMM-score: 234.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007644
    • TAT(Tat/SPI): 0.000741
    • LIPO(Sec/SPII): 0.001336
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRLVANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLKFRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTARHHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKVTSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQIGTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK

Experimental data[edit | edit source]

  • experimentally validated: see SACOL0777
  • protein localization: see SACOL0777
  • quantitative data / protein copy number per cell: see SACOL0777
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]