PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS06220 [old locus tag: SACOL1214 ]
• pan locus tag^?: SAUPAN003482000
• symbol: SACOL_RS06220
• pan gene symbol^?: carA
• synonym:
• product: carbamoyl-phosphate synthase small chain

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS06220 [old locus tag: SACOL1214 ]
• symbol: SACOL_RS06220
• product: carbamoyl-phosphate synthase small chain
• replicon: chromosome
• strand: +
• coordinates: 1221623..1222723
• length: 1101
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1221623..1222723) NCBI
• BioCyc: SACOL_RS06220 BioCyc
• MicrobesOnline: see SACOL1214

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCAAAGCAAACGTTATCTAGTGTTAGAAGACGGTTCTTTTTACGAAGGCTACCGTTTA
  GGATCTGATAACTTAACTGTAGGAGAAATTGTATTTAATACAGCGATGACAGGTTATCAA
  GAAACTATTTCAGATCCATCATATACAGGTCAGATCATTACTTTTACGTATCCATTAATC
  GGTAATTATGGTATCAATAGAGACGATTTTGAATCATTAGTACCTACATTAAACGGTATT
  GTAGTGAAAGAAGCGAGTGCGCATCCAAGTAATTTTAGACAGCAAAAGACACTTCATGAC
  GTTTTAGAATTGCATCAAATTCCAGGGATTGCAGGTGTTGATACAAGAAGTATTACGCGT
  AAAATTCGACAACACGGTGTGTTAAAAGCTGGTTTTACTGATCGAAAAGAAGATATTGAT
  CAACTTGTCAAACATTTACAACAAGTAGAATTACCTAAAAACGAAGTAGAAATCGTTTCG
  ACTAAAACACCGTATGTTTCGACAGGTAAGGATCTAAGTGTCGTACTTGTAGACTTTGGT
  AAGAAGCAAAATATTGTTCGAGAATTAAACGTCAGAGGTTGTAACGTCACAGTTGTACCA
  TATACAACTACTGCCGAAGAAATTTTAGCAATGGCTCCAGATGGCGTTATGCTATCAAAC
  GGACCAGGTAATCCTGAAGTTGTAGAATGTGCGATTCCAATGATTCAAGGAATTTTAGGG
  AAAATTCCGTTCTTTGGTATCTGTCTAGGACATCAACTTTTTGCATTATCTCAAGGAGCA
  AGCTCATTTAAAATGAAGTTTGGTCATCGTGGTGCGAACCATCCAGTTAAAAATTTAGAG
  ACTGGAAAAGTTGATATTACGAGTCAAAACCATGGATATGCAATAGATATAGATTCGTTA
  AAAAGTACTGATTTAGAAGTTACTCATCTTGCATTAAATGATGGTACTGTAGAAGGTTTA
  AAACATAAAACATTACCAGCATTTTCTGTTCAATACCATCCTGAAGCAAATCCAGGACCG
  TCAGATTCAAACTATCTATTTGATGATTTTGTAGCAATGATGACTAATTTTAAGGAAAAG
  GAGCGTCATATCAATGCCTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS06220 [old locus tag: SACOL1214 ]
• symbol: SACOL_RS06220
• description: carbamoyl-phosphate synthase small chain
• length: 366
• theoretical pI: 6.18896
• theoretical MW: 40393.6
• GRAVY: -0.25847

⊟Function

• reaction:
  EC 6.3.5.5?  ExPASy
  Carbamoyl-phosphate synthase (glutamine-hydrolyzing) 2 ATP + L-glutamine + HCO₃(-) + H₂O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 484.5)
  and 5 more
  glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 62.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 59.9)
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 50.1)
  aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 48.6)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 20)
• TheSEED: see SACOL1214
• PFAM:
  Leu-IlvD (CL0364) CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain (PF00988; HMM-score: 167.7)
  Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 162.1)
  and 1 more
  Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 30.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016429
  • TAT(Tat/SPI): 0.000433
  • LIPO(Sec/SPII): 0.00096
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447113657 NCBI
• RefSeq: WP_001190913 NCBI
• UniProt: see SACOL1214

⊟Protein sequence

• MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVLELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVSTKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEKERHINA

⊟Experimental data

• experimentally validated: see SACOL1214
• protein localization: see SACOL1214
• quantitative data / protein copy number per cell: see SACOL1214
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: pyrR leader see SACOL1214

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1214

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications