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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07300 [old locus tag: SACOL1433 ]
  • pan locus tag?: SAUPAN003811000
  • symbol: SACOL_RS07300
  • pan gene symbol?: dapI
  • synonym:
  • product: hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS07300 [old locus tag: SACOL1433 ]
  • symbol: SACOL_RS07300
  • product: hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1445356..1446507
  • length: 1152
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1445356..1446507) NCBI
  • BioCyc: SACOL_RS07300 BioCyc
  • MicrobesOnline: see SACOL1433

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATGAATTAGAATTTGTTACGAAACATCGCCGTCATTTACATCAACATCCTGAATTA
    AGCTTACATGAATTTGAAACAACTGCTTATATTAAAGCGTTTTTAGATAGTTTAAATATT
    AAATACGATTGCCCATTGGAAACTGGCGTCATTGCATACTTAGAAGGTAATGGCTCACAT
    ACGATAGCGTATAGAGCTGATATTGATGCGTTACCTATTTTAGAGGAAAATGATGTGCCT
    TATCGCAGTCAATCTGATCATGTGATGCATGCTTGTGGACATGATGGTCATACAACTGCA
    TTAATGCTTTTTGTACAACGTTGCAAAGACATGCAAGATGCAGGTCAATTACCGCAAAAT
    GTCGTTTTCATTTTCCAACCTGCAGAAGAAACTGGTGGCGGTGCAAATCGATTAATAAAA
    GCCGGTGCCTTTGATAAGTATCCAATTGAAGCGGTATTTGGTATTCATGTTAACCCATTT
    GCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTAT
    CGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGT
    GGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAAC
    GGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACT
    GTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACA
    ATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAT
    TGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGAAATTACGTACT
    CAAATAGAGGACGCCTTAATAAAAGCTGATTTAAATGTCTATGACAAACCAACGCCATTC
    TTATTTGGGGAAGATTTTAGTTTTTATGGTCAACAACTAGCTCCAGCTTACTTTGTTTTT
    ATAGGAACACGAAATGAAGATAAAGGTTTTGTAACTGGTTTGCACACATCACATTTAAAT
    TTTGATGAAAAAGTGTTAATAAACGTGATTAATTTTTACGAAAATTTATTAAATAATTAC
    AAAGAGGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1152

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS07300 [old locus tag: SACOL1433 ]
  • symbol: SACOL_RS07300
  • description: hydrolase
  • length: 383
  • theoretical pI: 5.35281
  • theoretical MW: 43157.4
  • GRAVY: -0.251436

⊟Function[edit | edit source]

  • reaction:
    EC 3.-.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 279.5)
    and 2 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 20.6)
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 20.4)
  • TheSEED: see SACOL1433
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 117.6)
    and 2 more
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 22.7)
    DUF1310; Protein of unknown function (DUF1310) (PF07006; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002731
    • TAT(Tat/SPI): 0.000328
    • LIPO(Sec/SPII): 0.000364
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDCPLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALMLFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPFADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFHLNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLLFNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLAPAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVINFYENLLNNYKEV

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1433
  • protein localization: see SACOL1433
  • quantitative data / protein copy number per cell: see SACOL1433
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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