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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07515 [old locus tag: SACOL1475 ]
  • pan locus tag?: SAUPAN003890000
  • symbol: SACOL_RS07515
  • pan gene symbol?: norB
  • synonym:
  • product: quinolone resistance protein NorB

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS07515 [old locus tag: SACOL1475 ]
  • symbol: SACOL_RS07515
  • product: quinolone resistance protein NorB
  • replicon: chromosome
  • strand: -
  • coordinates: 1511761..1513152
  • length: 1392
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1511761..1513152) NCBI
  • BioCyc: SACOL_RS07515 BioCyc
  • MicrobesOnline: see SACOL1475

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAAGCCGTCAAGAGAGGCATTTGAAGGCAATAATAAGTTGTTAATAGGAATTGTT
    CTAAGTGTAATAACGTTTTGGCTATTTGCACAATCATTGGTTAATGTTGTACCAATACTT
    GAAGATAGTTTCAATACAGATATTGGAACGGTTAATATCGCCGTTAGTATAACTGCTTTA
    TTTTCAGGAATGTTTGTAGTAGGAGCAGGTGGTCTTGCTGATAAATATGGCAGAATTAAA
    CTCACGAACATTGGTATTATCTTAAATATATTAGGTTCATTATTAATCATTATTTCAAAT
    ATTCCTTTATTACTTATTATAGGAAGATTAATTCAAGGACTTTCAGCAGCATGTATTATG
    CCTGCAACTTTGTCTATTATTAAGTCATATTACATTGGGAAAGATAGACAACGCGCTTTA
    AGTTATTGGTCAATTGGCTCATGGGGCGGCTCTGGTGTTTGTTCATTTTTTGGAGGTGCA
    GTTGCAACGCTTTTAGGTTGGCGTTGGATTTTCATCCTATCAATTATAATTTCATTAATT
    GCACTGTTTCTTATTAAAGGCACACCTGAAACTAAATCTAAATCGATTTCTCTAAATAAA
    TTTGACATTAAAGGTCTGGTTCTTTTAGTCATTATGCTCCTCAGTTTAAATATTTTAATT
    ACTAAAGGATCAGAATTAGGTGTAACCTCACTTCTTTTTATTACTTTATTAGCTATTGCA
    ATTGGATCTTTTAGTTTATTTATAGTTCTTGAAAAGCGTGCTACAAATCCTTTAATCGAT
    TTTAAATTATTTAAAAATAAAGCTTACACAGGTGCAACAGCTTCAAACTTTTTGTTAAAT
    GGTGTTGCAGGAACATTAATAGTAGCCAACACATTTGTTCAAAGAGGTTTAGGATATTCT
    TCATTGCAAGCAGGAAGTTTATCAATCACTTATTTAGTAATGGTACTAATTATGATTCGT
    GTTGGTGAAAAGTTACTTCAAACACTCGGATGCAAGAAACCAATGTTAATTGGAACAGGA
    GTTCTTATTGTCGGAGAATGTCTCATTTCATTAACTTTCTTGCCAGAAATATTCTATGTC
    ATTTGTTGTATTATAGGTTATTTATTCTTTGGTTTAGGACTAGGGATATATGCTACACCA
    TCAACAGATACAGCAATTGCAAATGCACCGTTAGAAAAAGTAGGCGTTGCTGCAGGTATC
    TATAAAATGGCTTCTGCATTAGGTGGAGCATTTGGCGTCGCATTGAGTGGTGCAGTATAT
    GCAATCGTATCAAATATGACAAACATTTATACAGGTGCAATGATTGCATTATGGTTAAAT
    GCAGCTATGGGAATATTATCATTCGTTATCATTTTGTTACTTGTGCCTAAACAAAACGAT
    ACTCAATTATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1392

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS07515 [old locus tag: SACOL1475 ]
  • symbol: SACOL_RS07515
  • description: quinolone resistance protein NorB
  • length: 463
  • theoretical pI: 9.70893
  • theoretical MW: 49306.9
  • GRAVY: 0.974946

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 132.4)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 132.4)
    and 7 more
    multidrug resistance protein (TIGR00880; HMM-score: 62.6)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 45.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 40.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 31.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 31.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 23.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 16.9)
  • TheSEED: see SACOL1475
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 178.9)
    and 1 more
    PUCC; PUCC protein (PF03209; HMM-score: 15.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.06735
    • TAT(Tat/SPI): 0.003796
    • LIPO(Sec/SPII): 0.018709
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGAVATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNILITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAAMGILSFVIILLLVPKQNDTQL

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]