PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS09245 [old locus tag: SACOL1804 ]
• pan locus tag^?: SAUPAN004432000
• symbol: SACOL_RS09245
• pan gene symbol^?: —
• synonym:
• product: polysaccharide biosynthesis protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS09245 [old locus tag: SACOL1804 ]
• symbol: SACOL_RS09245
• product: polysaccharide biosynthesis protein
• replicon: chromosome
• strand: -
• coordinates: 1852144..1853805
• length: 1662
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1852144..1853805) NCBI
• BioCyc: SACOL_RS09245 BioCyc
• MicrobesOnline: see SACOL1804

⊟Phenotype

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⊟DNA sequence

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  ATGAGTGAAAGTAAAGAAATGGTGCGTGGAACCTTTTTAATTACCATTAGTATATTAATT
  ACCAAGGTATTGGGCGTACTTTTTATCATTCCATTCAACTATTTAATTGGTGGACAAGAA
  AATATGGCGCCGTTCACATACGCTTATGCACCATATAATATTGCAATTGCAGTTGCGACA
  GCAGGTGTACCATTAGCAGCTTCGAAATATGTTGCGAAATATAATGCTATTGGGGCATAT
  AAAGTAAGTCAGAAATTTTATAAATCGAGTTTTATTGTAATGAGTATTACCGGTGTTTTA
  GGTTTCTTGGTACTGTATTTCTTAGCACCTTATATTTCTGAATTAACATTAGCTAGAAAC
  ATTCATGATAAAAACGGTTGGTCAGTCGATGATATTACTTGGATTATAAGAATCATTAGT
  ATGGTTGTTATCTTTATTCCTGTATTAGCGACATGGAGAGGGATATTCCAAGGTTATAAA
  TCTATGGGGCCAACAGCAGTGTCAGAAGTAACTGAGCAAATTGCACGCGTGATTTTCATA
  TTAATCGGAAGTTATTTAGTACTAAATGTCTTTGATGGCTCTATTTTATTAGCCAATGGT
  ATTGCTACTTTTGCAGCAGCAGTTGGTGCGATTATTGGTATATTCACACTCTGGTATTAC
  TGGAGAAAGCGTAAGCATAATATTGATCGTATGGTCGAATCTGATTACACAGATATAGAT
  GTGTCATATGGCAAGATGTATAAAGAAATTATTGCTTATAGTATACCGTTCGTTATTGTA
  AGTTTGAATTATCCATTATTTAACTTAGTAGATCAATTTACACATAACGGTGCTTTATCT
  TTAGTTGGTATACCTTCACAGTTACAAGATATATTCTTTAATATGCTAAATATGTCAACT
  AATAAAATTGTAATGATTCCGACATCATTAAGTGCTGGTTTTGCAGTGAGTTTAATTCCT
  TATATTACAAAAACATTTGCAGAAGGTCGCTTACATGAAATGCATCATCAAATTAGAACA
  TCTATTGGTGTATTAATGTTTATAACTGTTCCAGCTAGTATTGGAATTATGGCCTTGGCA
  CAACCATTATTCACAGTTTTCTATGGTTACGATCCAATTGTTTTAGGTCATGACCCTAAC
  CATGATGGTAGTCGCTTATTATTCTATTATGCGCCTGTAGCAATACTAATTTCACTATTA
  AGTGTAACGGCATCAATGTTACAAGGTATAGATAAACAGAAATTAACAGTTTATGTTATT
  TTAGCTTCTGTAGTTATCAAGTTAGCGTTAAACTATCCATTAATTATGTTATTCCATACA
  CCTGGTGCAATTTTAAGTACTAGTATCGCATTATTATTTGCAATTGGATGTAATTTCTAT
  ATTCTTAAAAAATATGCGAAGTTTAAATTCAGCTATAGTTGGATTCATTTTGCAAAAATA
  TTCTTGTATTCATTTATTATGATGCTAGGTGTAGAATTAGTATTTTTCCTTGCAAATCTA
  TTCTTAGAACCTACTAAACTTGGTTACTTAATCATTATTATTTTAGGTGTTACAGTAGGT
  ATTCTAATTTACGGTACAATCACAATTAAAACGCGTCTTGCGGACGAATTCTTAGGTGAA
  ATTCCTGAAAAATTAAGACGTAGAGTTAGGTTTTTACGATGA
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⊟Protein

⊟General

• locus tag: SACOL_RS09245 [old locus tag: SACOL1804 ]
• symbol: SACOL_RS09245
• description: polysaccharide biosynthesis protein
• length: 553
• theoretical pI: 9.70447
• theoretical MW: 61945.3
• GRAVY: 0.712116

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination stage V sporulation protein B (TIGR02900; HMM-score: 122.7)
  and 2 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan murein biosynthesis integral membrane protein MurJ (TIGR01695; HMM-score: 43.5)
  Metabolism Transport and binding proteins Other MATE efflux family protein (TIGR00797; HMM-score: 21.5)
• TheSEED: see SACOL1804
• PFAM:
  MviN_MATE (CL0222) Polysacc_synt; Polysaccharide biosynthesis protein (PF01943; HMM-score: 103.8)
  Polysacc_synt_C; Polysaccharide biosynthesis C-terminal domain (PF14667; HMM-score: 92.1)
  and 3 more
  MatE; MatE (PF01554; HMM-score: 42.2)
  MVIN; MviN-like protein (PF03023; HMM-score: 37.4)
  Holin-II (CL0563) Phage_holin_2_4; Bacteriophage holin family, superfamily II-like (PF16082; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.043737
  • TAT(Tat/SPI): 0.000861
  • LIPO(Sec/SPII): 0.002372
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 445926480 NCBI
• RefSeq: WP_000004335 NCBI
• UniProt: see SACOL1804

⊟Protein sequence

• MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAPFTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGFLVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYKSMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLWYYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHNGALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEMHHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAPVAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIALLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLGYLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR

⊟Experimental data

• experimentally validated: see SACOL1804
• protein localization: see SACOL1804
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications