From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09245 [old locus tag: SACOL1804 ]
  • pan locus tag?: SAUPAN004432000
  • symbol: SACOL_RS09245
  • pan gene symbol?:
  • synonym:
  • product: polysaccharide biosynthesis protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS09245 [old locus tag: SACOL1804 ]
  • symbol: SACOL_RS09245
  • product: polysaccharide biosynthesis protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1852144..1853805
  • length: 1662
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1852144..1853805) NCBI
  • BioCyc: SACOL_RS09245 BioCyc
  • MicrobesOnline: see SACOL1804

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGTGAAAGTAAAGAAATGGTGCGTGGAACCTTTTTAATTACCATTAGTATATTAATT
    ACCAAGGTATTGGGCGTACTTTTTATCATTCCATTCAACTATTTAATTGGTGGACAAGAA
    AATATGGCGCCGTTCACATACGCTTATGCACCATATAATATTGCAATTGCAGTTGCGACA
    GCAGGTGTACCATTAGCAGCTTCGAAATATGTTGCGAAATATAATGCTATTGGGGCATAT
    AAAGTAAGTCAGAAATTTTATAAATCGAGTTTTATTGTAATGAGTATTACCGGTGTTTTA
    GGTTTCTTGGTACTGTATTTCTTAGCACCTTATATTTCTGAATTAACATTAGCTAGAAAC
    ATTCATGATAAAAACGGTTGGTCAGTCGATGATATTACTTGGATTATAAGAATCATTAGT
    ATGGTTGTTATCTTTATTCCTGTATTAGCGACATGGAGAGGGATATTCCAAGGTTATAAA
    TCTATGGGGCCAACAGCAGTGTCAGAAGTAACTGAGCAAATTGCACGCGTGATTTTCATA
    TTAATCGGAAGTTATTTAGTACTAAATGTCTTTGATGGCTCTATTTTATTAGCCAATGGT
    ATTGCTACTTTTGCAGCAGCAGTTGGTGCGATTATTGGTATATTCACACTCTGGTATTAC
    TGGAGAAAGCGTAAGCATAATATTGATCGTATGGTCGAATCTGATTACACAGATATAGAT
    GTGTCATATGGCAAGATGTATAAAGAAATTATTGCTTATAGTATACCGTTCGTTATTGTA
    AGTTTGAATTATCCATTATTTAACTTAGTAGATCAATTTACACATAACGGTGCTTTATCT
    TTAGTTGGTATACCTTCACAGTTACAAGATATATTCTTTAATATGCTAAATATGTCAACT
    AATAAAATTGTAATGATTCCGACATCATTAAGTGCTGGTTTTGCAGTGAGTTTAATTCCT
    TATATTACAAAAACATTTGCAGAAGGTCGCTTACATGAAATGCATCATCAAATTAGAACA
    TCTATTGGTGTATTAATGTTTATAACTGTTCCAGCTAGTATTGGAATTATGGCCTTGGCA
    CAACCATTATTCACAGTTTTCTATGGTTACGATCCAATTGTTTTAGGTCATGACCCTAAC
    CATGATGGTAGTCGCTTATTATTCTATTATGCGCCTGTAGCAATACTAATTTCACTATTA
    AGTGTAACGGCATCAATGTTACAAGGTATAGATAAACAGAAATTAACAGTTTATGTTATT
    TTAGCTTCTGTAGTTATCAAGTTAGCGTTAAACTATCCATTAATTATGTTATTCCATACA
    CCTGGTGCAATTTTAAGTACTAGTATCGCATTATTATTTGCAATTGGATGTAATTTCTAT
    ATTCTTAAAAAATATGCGAAGTTTAAATTCAGCTATAGTTGGATTCATTTTGCAAAAATA
    TTCTTGTATTCATTTATTATGATGCTAGGTGTAGAATTAGTATTTTTCCTTGCAAATCTA
    TTCTTAGAACCTACTAAACTTGGTTACTTAATCATTATTATTTTAGGTGTTACAGTAGGT
    ATTCTAATTTACGGTACAATCACAATTAAAACGCGTCTTGCGGACGAATTCTTAGGTGAA
    ATTCCTGAAAAATTAAGACGTAGAGTTAGGTTTTTACGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS09245 [old locus tag: SACOL1804 ]
  • symbol: SACOL_RS09245
  • description: polysaccharide biosynthesis protein
  • length: 553
  • theoretical pI: 9.70447
  • theoretical MW: 61945.3
  • GRAVY: 0.712116

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein B (TIGR02900; HMM-score: 122.7)
    and 2 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan murein biosynthesis integral membrane protein MurJ (TIGR01695; HMM-score: 43.5)
    Metabolism Transport and binding proteins Other MATE efflux family protein (TIGR00797; HMM-score: 21.5)
  • TheSEED: see SACOL1804
  • PFAM:
    MviN_MATE (CL0222) Polysacc_synt; Polysaccharide biosynthesis protein (PF01943; HMM-score: 103.8)
    Polysacc_synt_C; Polysaccharide biosynthesis C-terminal domain (PF14667; HMM-score: 92.1)
    and 3 more
    MatE; MatE (PF01554; HMM-score: 42.2)
    MVIN; MviN-like protein (PF03023; HMM-score: 37.4)
    Holin-II (CL0563) Phage_holin_2_4; Bacteriophage holin family, superfamily II-like (PF16082; HMM-score: 13)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.043737
    • TAT(Tat/SPI): 0.000861
    • LIPO(Sec/SPII): 0.002372
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAPFTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGFLVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYKSMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLWYYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHNGALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEMHHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAPVAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIALLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLGYLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR

Experimental data[edit | edit source]

  • experimentally validated: see SACOL1804
  • protein localization: see SACOL1804
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]