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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09800 [old locus tag: SACOL1903 ]
  • pan locus tag?: SAUPAN004777000
  • symbol: SACOL_RS09800
  • pan gene symbol?:
  • synonym:
  • product: DUF445 domain-containing protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS09800 [old locus tag: SACOL1903 ]
  • symbol: SACOL_RS09800
  • product: DUF445 domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1958239..1959363
  • length: 1125
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1958239..1959363) NCBI
  • BioCyc: SACOL_RS09800 BioCyc
  • MicrobesOnline: see SACOL1903

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAATGCACTATTTATTATCATATTTATGATTGTAGTAGGGGCGATAATTGGTGGCATT
    ACTAATGTAATTGCAATTAGAATGCTGTTTCACCCCTTTAAACCATACTATATATTTAAA
    TTTAGAGTCCCATTTACACCAGGTTTAATACCGAAAAGACGCGAAGAAATTGCAACTAAA
    ATTGGCCAAGTGATTGAAGAGCATTTGCTTACAGAAACTTTAATTAATGAAAAATTGAAA
    AGCGAGCAATCACAGCAAGCAATAGAATCTATGATTCAACAGCAGTTACAAAAGTTGACG
    AAAGATCAATTGTCAATAAAACAAATTACTTCTCAAATCGATATTGATTTAGAACAAGTA
    TTACAAACTAATGGAAATCAATATATTGAATCACAATTGAATAATTATTATACAAAGCAT
    CAAAACCAAACAATAGCATCTTTATTGCCAAATCAACTTGTAACATTTTTAAATCAGCAT
    GTAGATAACGCAACAGACTTATTATGTGATCGTGCAAGGAATTATTTATCATCTGCAAAA
    GGCACACAAGATATTAATGATATGTTGGATACTTTTTTCAATGAGAAAGGTAAGTTAATT
    GGTATGTTGCAAATGTTTATGACAAAAGAGAGTATTGCAGATCGCATTCAACAAGAACTT
    ATACGTTTAACATCTCATCCTAAAGCAAGAACAATTGTGACATCGTTAATTACCAATGAA
    TATCAAACTTTTAAAGATAAACCATTGAATGAACTGTTAGATGCATCGCAGTTTAATGAA
    ATCGCTGAAAATCTATCAGTGTATGTAACAACATATGCAAGCAAACAAGCGAATAAGCCA
    GTGGTTACGTTAATGCCTCAATTCGTTGATTATTTAGAAGGTCAACTCTCTAGCAAATTA
    GCAAATTTAATCATTGAAAAATTATCGATTCACTTATCTACAATTATGAAAAAAGTGGAT
    TTACGAGGATTAATTGAAGAACAAATTAATACGTTTGACTTAGATTATATTGAAAAATTA
    ATTATAGAAATTGCTAACAAAGAACTAAAGTTGATTATGTCATTAGGTTTCATTTTAGGT
    GGTATTATTGGATTTTTCCAAGGTTTGGTTGCAATCTTTGTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS09800 [old locus tag: SACOL1903 ]
  • symbol: SACOL_RS09800
  • description: DUF445 domain-containing protein
  • length: 374
  • theoretical pI: 6.26855
  • theoretical MW: 42695.4
  • GRAVY: 0.0117647

Function[edit | edit source]

  • TIGRFAM:
    conserved hypothetical protein (TIGR04540; HMM-score: 5.6)
    6-pyruvoyl tetrahydropterin synthase-related domain (TIGR03112; HMM-score: 4.9)
  • TheSEED: see SACOL1903
  • PFAM:
    no clan defined DUF445; Protein of unknown function (DUF445) (PF04286; HMM-score: 252.7)
    and 4 more
    HUP (CL0039) BshC; Bacillithiol biosynthesis BshC (PF10079; HMM-score: 16.3)
    Dim_A_B_barrel (CL0032) Cyclase_polyket; Polyketide synthesis cyclase (PF04673; HMM-score: 12.4)
    no clan defined MukF_C; MukF C-terminal domain (PF17193; HMM-score: 10.3)
    Nexin_C; Sorting nexin C terminal (PF08628; HMM-score: 9.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.066344
    • TAT(Tat/SPI): 0.001417
    • LIPO(Sec/SPII): 0.042124
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVPFTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKDQLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQHVDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDLDYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV

Experimental data[edit | edit source]

  • experimentally validated: see SACOL1903
  • protein localization: see SACOL1903
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]