PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS10665 [old locus tag: SACOL2042 ]
• pan locus tag^?: SAUPAN005303000
• symbol: SACOL_RS10665
• pan gene symbol^?: ilvD
• synonym:
• product: dihydroxy-acid dehydratase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS10665 [old locus tag: SACOL2042 ]
• symbol: SACOL_RS10665
• product: dihydroxy-acid dehydratase
• replicon: chromosome
• strand: +
• coordinates: 2101069..2102757
• length: 1689
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2101069..2102757) NCBI
• BioCyc: SACOL_RS10665 BioCyc
• MicrobesOnline: see SACOL2042

⊟Phenotype

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⊟DNA sequence

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  ATGCGAAGCGACATGATCAAAAAAGGAGATCACCAAGCACCAGCAAGAAGTCTTTTACAT
  GCCACGGGCGCGCTAAAAAGTCCAACTGATATGAACAAACCATTTGTAGCTATTTGTAAC
  TCTTATATTGATATTGTTCCTGGACATGTTCACTTGAGAGAGCTTGCAGATATAGCTAAA
  GAAGCAATTAGAGAAGCCGGTGCCATTCCATTTGAATTCAATACAATTGGTGTTGATGAT
  GGAATAGCTATGGGACATATCGGAATGCGATATTCTCTACCATCACGTGAAATTATTGCA
  GATGCAGCTGAAACTGTAATTAACGCTCATTGGTTTGACGGCGTATTTTACATTCCTAAT
  TGTGACAAGATTACACCCGGTATGATTTTAGCAGCCATGAGGACAAACGTACCAGCTATC
  TTTTGCTCTGGTGGACCAATGAAAGCTGGCTTATCTGCACATGGAAAAGCATTAACACTT
  TCATCAATGTTTGAAGCAGTCGGCGCATTTAAAGAAGGATCGATTTCTAAAGAAGAATTT
  TTAGATATGGAACAAAATGCCTGCCCTACTTGTGGTTCATGTGCTGGGATGTTTACTGCA
  AATTCAATGAACTGTTTGATGGAAGTTTTAGGTCTAGCATTACCATACAACGGTACTGCA
  CTTGCAGTCAGTGATCAGCGACGAGAAATGATTCGCCAAGCAGCTTTTAAATTAGTTGAA
  AATATTAAAAATGATTTAAAACCACGTGATATCGTTACTCGCGAAGCAATTGATGATGCA
  TTTGCACTTGATATGGCTATGGGTGGTTCAACAAACACGGTACTGCATACGTTAGCCATT
  GCCAATGAAGCTGGTATTGATTATGACTTAGAGCGCATTAATGCTATTGCCAAACGCACG
  CCATATTTATCAAAAATAGCACCTAGTTCATCGTATTCAATGCATGATGTGCATGAAGCT
  GGTGGCGTCCCAGCAATTATTAATGAATTGATGAAGAAAGATGGCACGTTACACCCAGAT
  AGAATCACAGTTACTGGCAAAACGTTACGTGAAAATAACGAAGGCAAAGAAATTAAGAAC
  TTTGATGTCATTCACCCTCTTGATGCACCATATGATGCACAAGGCGGTTTATCTATCTTA
  TTTGGTAATATCGCCCCTAAAGGCGCAGTTATTAAAGTTGGCGGCGTTGATCCATCTATC
  AAAACATTTACTAGGAAAGCAATTTGTTTCAATTCGCATGATGAAGCTGTTGAAGCAATA
  GACAATCGTACCGTTCGTGCAGGCCACGTCGTTGTCATTAGATATGAAGGACCTAAAGGT
  GGACCAGGTATGCCTGAAATGTTAGCACCTACTTCCTCTATTGTTGGTCGCGGCTTAGGT
  AAAGATGTTGCATTAATTACTGATGGGCGTTTTTCCGGTGCCACAAGAGGTATTGCAGTT
  GGTCATATTTCCCCTGAAGCTGCATCTGGTGGACCAATTGCCTTAATTGAAGATGGTGAT
  GAGATTACTATTGATTTAACAAATCGTACATTAAACGTAAACCAGCCTGAAGATGTTCTA
  GCGCGTCGCCGAGAATCTTTAACACCATTTAAAGCGAAAGTAAAAACAGGTTATCTAGCT
  CGTTATACTGCCCTAGTAACTAGCGCAAATACAGGTGGCGTCATGCAAGTCCCTGAGAAT
  TTAATTTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS10665 [old locus tag: SACOL2042 ]
• symbol: SACOL_RS10665
• description: dihydroxy-acid dehydratase
• length: 562
• theoretical pI: 6.35197
• theoretical MW: 60120.6
• GRAVY: -0.0613879

⊟Function

• reaction:
  EC 4.2.1.9?  ExPASy
  Dihydroxy-acid dehydratase 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H₂O
• TIGRFAM:
  Metabolism Amino acid biosynthesis Pyruvate family dihydroxy-acid dehydratase (TIGR00110; EC 4.2.1.9; HMM-score: 779.7)
  and 2 more
  Metabolism Energy metabolism Entner-Doudoroff phosphogluconate dehydratase (TIGR01196; EC 4.2.1.12; HMM-score: 285.2)
  Unknown function Enzymes of unknown specificity putative dehydratase, YjhG/YagF family (TIGR03432; HMM-score: 220.3)
• TheSEED: see SACOL2042
• PFAM:
  no clan defined ILVD_EDD; Dehydratase family (PF00920; HMM-score: 731.9)
  and 1 more
  NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: [4Fe-4S] cluster
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013615
  • TAT(Tat/SPI): 0.0042
  • LIPO(Sec/SPII): 0.004851
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447178526 NCBI
• RefSeq: WP_001255782 NCBI
• UniProt: see SACOL2042

⊟Protein sequence

• MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYIDIVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADAAETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTLSSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNGTALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLHTLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKDGTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIKVGGVDPSIKTFTRKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTNRTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI

⊟Experimental data

• experimentally validated: see SACOL2042
• protein localization: see SACOL2042
• quantitative data / protein copy number per cell: see SACOL2042
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SACOL2042

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL2042

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications