From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS11400 [old locus tag: SACOL2168 ]
  • pan locus tag?: SAUPAN005569000
  • symbol: SACOL_RS11400
  • pan gene symbol?: sfnaC
  • synonym:
  • product: alanine racemase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS11400 [old locus tag: SACOL2168 ]
  • symbol: SACOL_RS11400
  • product: alanine racemase
  • replicon: chromosome
  • strand: -
  • coordinates: 2247238..2248308
  • length: 1071
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2247238..2248308) NCBI
  • BioCyc: SACOL_RS11400 BioCyc
  • MicrobesOnline: see SACOL2168

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCACACGTTAACATAAATATATCGAAGATTAAATATAACGCCAAAGTACTTCAAACA
    GTTTTTCAAAGTAAAAATATGCAATTCACACCAGTAATTAAGTGCATAGCTGGTGACCGT
    ACAATTGTAGAAAGCTTAAAAGCGTTAGGTATCAATCATGTTGCAGAATCCAGATTGGAT
    AACATAATTAGTATTGCAGATCAGGATTTAACATATACGTTATTGCGTACGCCTGCCAAA
    AAAGAGATTTCAGATATGATAGAAAAAGTTGATATGAGTATCCAAACAGAATTATCTACC
    ATACATCAAATAAATGAAGTAGCAGAAGTTTTAGGAAAAAAACATAAGATTTTATTAATG
    GTAGATTGGAAAGATGGCCGTGAAGGTGTTCTGACTTATGATGTTTTAGATTATATTAAA
    GAGATTATTCACTTGAAAAATATCCATTTTGTAGGTTTAGCATTCAATTTTATGTGTTTT
    AAATCTGATGCGCCAAGTGATGATGATATCTTTATGATTAATCGATTCGTCTCAGCTGTT
    GAAAGAGAAATAGGATATCGATTGAAAATTATTTCAGGTGGTAATTCGAGCATGTTACCA
    CAACTTTTATATAATGATTTGGGTAAAATAAATGAATTGCGAATAGGTGAAACGTTGTTT
    AGAGGTGTTGATACAACGACAAATCAAGCGATCGCTATGTTATATCAAGATGCGATTACA
    TTGGAGGCTGAGATTTTAGAAATTAAACCTCGAGTTAATACACAAACGCATGAATCATTC
    TTACAAGCTATTGTAGATATTGGTTATTTAGATACAAAAGTCGATAATATTTCACCTATG
    GATCAGCATATCAATATTTTAGGCGCTTCGAGTGATCATTTAATGCTTGATTTAAATGGT
    CAAGGACACTATCAAGTTGGAGATCATATATCTTTTTCATTAAATTATGAAGCACTTTCT
    CATTCGATGTATATGAAAAATTTACATAAGGTATATATTGATGATTCAAAGATTGATACG
    CTACTTCAAAATTTTGATGTGAAATCTCCTGCTATGGTAAACCACTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1071

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS11400 [old locus tag: SACOL2168 ]
  • symbol: SACOL_RS11400
  • description: alanine racemase
  • length: 356
  • theoretical pI: 5.85992
  • theoretical MW: 40348.2
  • GRAVY: -0.0932584

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan alanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 32.3)
  • TheSEED: see SACOL2168
  • PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 72)
    and 1 more
    no clan defined CPV_Polyhedrin; Cypovirus polyhedrin protein (PF17515; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002213
    • TAT(Tat/SPI): 0.000125
    • LIPO(Sec/SPII): 0.000462
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVESLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIHQINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCFKSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGETLFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDNISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYIDDSKIDTLLQNFDVKSPAMVNHY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL2168
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS11400&oldid=98648"