PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS13585 [old locus tag: SACOL2594 ]
• pan locus tag^?: SAUPAN006253000
• symbol: SACOL_RS13585
• pan gene symbol^?: gbaB
• synonym:
• product: short-chain dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS13585 [old locus tag: SACOL2594 ]
• symbol: SACOL_RS13585
• product: short-chain dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 2655607..2656311
• length: 705
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2655607..2656311) NCBI
• BioCyc: SACOL_RS13585 BioCyc
• MicrobesOnline: see SACOL2594

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACTAAAATTGTGTTAATCACAGGAGGCAATAAAGGGTTAGGCTATGCAAGTGCAGAG
  GCACTTAAAGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAA
  CAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCA
  GTCAAAAATGCTTATAACATGATTGCTGAAAAAGAAGGCCGTCTTGATATCCTCATTAAC
  AATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAA
  GAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGATGAATACATTCGTCCCTCTC
  TTAGAAAAATCTGAACAACCTGTTGTCGTCAACGTATCAAGTGGTTTAGGTTCATTTGGA
  ATGGTTACAAACCCCGAAACAGCTGAATCTAAAGTGAATTCATTAGCTTATTGTTCGTCT
  AAATCGGCAGTAACAATGCTAACGCTACAATACGCTAAAGGATTACCTAACATGCAAATC
  AATGCTGCTGATCCAGGCGCTACAAATACTGATTTAGTTGGTGATTTTAGTAATAATTCC
  AAACACGTTTCTGAAGGAATCAAGCCAATCATTCAATTAGCAACGATTGGTGCAGATGGC
  CCAACAGGTACATTTATTAATGGCGATGGAGAGATGCCTTGGTAG
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⊟Protein

⊟General

• locus tag: SACOL_RS13585 [old locus tag: SACOL2594 ]
• symbol: SACOL_RS13585
• description: short-chain dehydrogenase
• length: 234
• theoretical pI: 5.80622
• theoretical MW: 24931.1
• GRAVY: -0.126923

⊟Function

• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 79.9)
  2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 79)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 69)
  Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 64.8)
  acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 64.1)
  and 12 more
  3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 62.9)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 58.1)
  cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 49.1)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 47.3)
  2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 42.7)
  rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 36.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-dependent protochlorophyllide reductase (TIGR01289; EC 1.3.1.33; HMM-score: 34.4)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 33.3)
  sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 26.4)
  pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 16)
  Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 13.5)
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 12.1)
• TheSEED: see SACOL2594
• PFAM:
  NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 135.4)
  and 6 more
  adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 93)
  KR; KR domain (PF08659; HMM-score: 39.4)
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 17.6)
  ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 15.1)
  no clan defined PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 14.5)
  GT-B (CL0113) Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.030131
  • TAT(Tat/SPI): 0.001482
  • LIPO(Sec/SPII): 0.004751
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446083974 NCBI
• RefSeq: WP_000161829 NCBI
• UniProt: see SACOL2594

⊟Protein sequence

• MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQKLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEVYQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSSKSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGADGPTGTFINGDGEMPW

⊟Experimental data

• experimentally validated: see SACOL2594
• protein localization: see SACOL2594
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications