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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS13905 [old locus tag: SACOL2655 ]
- pan locus tag?: SAUPAN006362000
- symbol: SACOL_RS13905
- pan gene symbol?: arcD
- synonym:
- product: arginine-ornithine antiporter
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS13905 [old locus tag: SACOL2655 ]
- symbol: SACOL_RS13905
- product: arginine-ornithine antiporter
- replicon: chromosome
- strand: -
- coordinates: 2715888..2717318
- length: 1431
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1381ATGAACGAATCAGGAGATAACAAACTCAGTAAATCTTCTTTAATTGGACTAGTTATAGGA
TCCATGATTGGTGGCGGTGCGTTCAATATAATGTCTGATATGGGCGGTAAAGCCGGTGGA
TTAGCCATTATTATTGGTTGGATTATTACAGCTATAGGAATGATTTCATTAGCGTTCGTA
TTTCAAAATTTAACCAATGAACGGCCGGAGCTAGACGGTGGTATTTATAGTTATGCTCAA
GCAGGATTTGGCGATTTTGTAGGATTTATCAGTGCTTGGGGATATTGGTTCTCAGCGTTT
TTAGGCAATGTTGCCTATGCAACACTATTGATGTCAGCAGTAGGTAACTTTTTCCCGATT
TTTAAAGGAGGCAACACATTACCAAGTGTTATTGTCGCCTCGTTACTACTCTGGGGTGTC
CATTTCTTGATTTTAAAAGGCGTTGAAACAGCAGCATTTATCAATAGTATTGTTACTGTT
GCAAAGTTAATACCGATTTTACTTGTAATCATATGCATGATAATTGCATTCAATTTTGAC
ACTTTTAAAACAGGCTTTTTCAGTATGACGTCAGAGGGTGTATTGCCATTTAGTTGGGCG
AGCACAATGAGCCAAGTTAAAAGTACGATGCTAGTGACAGTTTGGGTGTTTATCGGTATC
GAAGGTGCAGTAATTTTTTCTAGTAGAGCTAAAAATAAAAAAGATGTAGGTAGTGCCACG
GTTATAGGACTTATATCAGTTTTAATTATCTATTTCTTATTAACTGTATTAGCTCAAGGC
GTGATTTTGCAAAATCATATTTCGCAATTAGATTCGCCAAGTATGGCACAGGTGCTTGCA
ACTATTGTAGGTGGTTGGGGATCTACACTTGTAAATATTGGTTTAATTATTTCGGTACTA
GGTGCATGGTTAGGATGGACACTGCTTGCTGGTGAATTACCTTTCATTGTTGCAAAAGAT
GGATTATTTCCAAAATGGTTTGCTAAAGAAAATAAAAATGGAGCACCTGTAAATGCACTG
CTTATTACCAATATATTAGTACAATTATTTTTAATAAGTATGCTATTTACACAGAGTGCG
TATCAATTTGCATTTTCACTAGCATCAAGTGCTATTTTATACCCTTACATGTTCAGTGCA
TTTTACCAAGTTAAATACACTTTAGAGCATCGACAGCAAGCAACTACTAAACAATGGACG
ATTGGTATCATAGCCTCAATTTATGCTATATGGCTTATATATGCAGCAGGTATCAATTAC
TTATTATTGACGATGTTACTTTATATTCCAGCTCTTCTTGTTTATACAATCGTTCAAAAG
AATAATCAGACACGTTTGATTAAATCAGACTATATTCTTTTTATGATTATTATCGTACTT
GCAGTTATCGGGTTAATTAAGTTATTGATGGGAACGATAAATGTTTTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS13905 [old locus tag: SACOL2655 ]
- symbol: SACOL_RS13905
- description: arginine-ornithine antiporter
- length: 476
- theoretical pI: 9.50412
- theoretical MW: 51716.2
- GRAVY: 0.907983
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 619.7)Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 568.9)and 8 morehistidine-histamine antiporter (TIGR04298; HMM-score: 140)Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 99)putrescine-ornithine antiporter (TIGR04299; HMM-score: 81.7)Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 59.2)Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 51.9)putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 46.1)Transport and binding proteins Amino acids, peptides and amines glutamate:gamma-aminobutyrate antiporter (TIGR00910; HMM-score: 24.4)Transport and binding proteins Amino acids, peptides and amines spore germination protein (amino acid permease) (TIGR00912; HMM-score: 21.5)
- TheSEED: see SACOL2655
- PFAM: APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 240.8)and 4 moreAA_permease; Amino acid permease (PF00324; HMM-score: 84.2)Spore_permease; Spore germination protein (PF03845; HMM-score: 30.4)no clan defined YwiC; YwiC-like protein (PF14256; HMM-score: 17.9)Gemini_mov; Geminivirus putative movement protein (PF01708; HMM-score: 12.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 13
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.012824
- TAT(Tat/SPI): 0.000841
- LIPO(Sec/SPII): 0.020508
- predicted transmembrane helices (TMHMM): 14
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAIIIGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLGNVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTVAKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFIGIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMAQVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNGAPVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQQATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSDYILFMIIIVLAVIGLIKLLMGTINVF
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: ArcR*, ArgR/AhrC, CcpA, Rex* see SACOL2655
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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