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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS14015 [old locus tag: SACOL2676 ]
  • pan locus tag?: SAUPAN006390000
  • symbol: SACOL_RS14015
  • pan gene symbol?: sasA
  • synonym:
  • product: serine-rich repeat glycoprotein adhesin SasA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS14015 [old locus tag: SACOL2676 ]
  • symbol: SACOL_RS14015
  • product: serine-rich repeat glycoprotein adhesin SasA
  • replicon: chromosome
  • strand: -
  • coordinates: 2748105..2754890
  • length: 6786
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2748105..2754890) NCBI
  • BioCyc: SACOL_RS14015 BioCyc
  • MicrobesOnline: see SACOL2676

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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    ATGAGTAAAAGACAGAAAGCATTTCATGACAGCTTAGCAAACGAAAAAACAAGAGTAAGA
    CTTTATAAATCTGGAAAAAATTGGGTAAAATCCGGAATTAAAGAAATAGAAATGTTCAAA
    ATTATGGGGCTACCATTTATTAGTCATAGTTTAGTGAGTCAAGATAATCAAAGCATTAGT
    AAAAAAATGACGGGATACGGACTGAAAACTACGGCGGTTATTGGTGGTGCATTCACGGTA
    AATATGTTGCATGACCAGCAAGCTTTTGCGGCTTCTGATGCACCATTAACTTCTGAATTA
    AACACACAAAGTGAAACAGTAGGTAATCAAAACTCAACGACAATCGAAGCATCAACATCA
    ACAGCCGATTCCACAAGTGTAACGAAAAATAGTAGTTCGGTACAAACATCAAATAGTGAC
    ACAGTCTCAAGTGAAAAGTCTGAAAAGGTCACTTCGACAACTAATAGTACAAGCAATCAA
    CAAGAGAAATTGACATCTACATCAGAATCAACATCCTCAAAGAATACTACATCAAGTTCT
    GATACTAAATCTGTAGCTTCAACTTCAAGTACAGAACAACCAATTAATACATCAACAAAT
    CAAAGTACTGCATCAAATAACACTTCACAAAGCACAACGCCATCTTCGGTCAACTTAAAC
    AAAACTAGCACAACGTCAACTAGCACCGCACCAGTAAAACTTCGAACTTTCAGTCGCTTA
    GCTATGTCAACATTTGCGTCAGCAGCGACGACAACCGCAGTAACTGCTAATACAATTACA
    GTTAATAAAGATAACTTAAAACAATATATGACAACGTCAGGTAATGCTACCTATGATCAA
    AGTACCGGTATTGTGACGTTAACACAGGATGCATACAGCCAAAAAGGTGCTATTACATTA
    GGAACACGTATTGACTCTAATAAGAGTTTTCATTTTTCTGGAAAAGTAAATTTAGGTAAC
    AAATATGAAGGGCATGGAAATGGTGGAGATGGTATCGGTTTTGCCTTTTCACCAGGTGTA
    TTAGGTGAAACAGGGTTAAACGGTGCCGCAGTAGGTATTGGTGGCTTAAGTAACGCATTT
    GGCTTCAAATTGGATACGTATCACAATACATCTAAACCAAATTCAGCTGCAAAGGCGAAT
    GCTGACCCATCTAATGTAGCTGGTGGAGGTGCGTTTGGTGCATTTGTAACAACAGATAGT
    TATGGTGTTGCGACAACGTATACATCAAGTTCAACAGCTGATAATGCTGCGAAGTTAAAT
    GTTCAACCTACAAATAACACGTTCCAAGATTTTGATATTAACTATAATGGTGATACAAAG
    GTTATGACTGTCAAATATGCAGGTCAAACATGGACACGTAATATTTCAGATTGGATTGCG
    AAAAGTGGTACGACCAACTTTTCATTATCAATGACAGCCTCAACAGGTGGCGCGACAAAT
    TTACAACAAGTACAATTTGGAACATTCGAATATACAGAGTCTGCTGTTACACAAGTGAGA
    TACGTTGATGTAACAACAGGTAAAGATATTATTCCACCAAAAACATATTCAGGAAATGTT
    GATCAAGTCGTGACAATCGATAATCAGCAATCTGCATTGACTGCTAAAGGATATAACTAC
    ACGTCCGTCGATAGTTCATATGCGTCAACTTATAATGATACAAATAAAACTGTAAAAATG
    ACGAATGCTGGACAATCAGTGACATATTATTTTACTGATGTAAAAGCACCAACTGTAACT
    GTAGGCAATCAAACCATAGAAGTGGGTAAAACAATGAATCCTATTGTATTGACTACAACG
    GATAATGGTACTGGGACTGTGACAAATACAGTTACAGGATTACCAAGCGGATTAAGTTAC
    GATAGTGCAACGAATTCAATCATTGGGACACCAACAAAAATTGGTCAATCAACAGTGACA
    GTTGTGTCTACTGACCAAGCAAATAACAAATCGACGACAACTTTTACAATAAATGTTGTG
    GATACGACAGCACCAACAGTGACACCAATAGGAGATCAATCATCAGAAGTGTATTCACCA
    ATATCCCCGATTAAAATTGCTACGCAAGATAACAGTGGAAATGCGGTGACGAATACAGTG
    ACTGGATTGCCATCCGGACTAACATTTGATAGTACAAATAATACTATTAGTGGTACACCA
    ACAAACATTGGTACAAGTACTATATCAATCGTTTCTACAGATGCGAGCGGTAACAAAACG
    ACGACAACTTTTAAATATGAAGTAACAAGAAATAGCATGAGTGATTCCGTATCAACATCA
    GGAAGTACACAACAATCTCAAAGTGTGTCAACAAGTAAAGCTGACTCACAAAGTGCATCA
    ACGAGTACATCAGGATCGATTGTGGTATCTACATCAGCTAGTACCTCGAAATCGACAAGT
    GTAAGCCTATCTGATTCTGTGAGTGCATCTAAGTCATTAAGCACATCTGAAAGTAATAGT
    GTATCAAGCTCAACAAGCACAAGTTTAGTGAATTCACAAAGTGTATCATCAAGCATGTCG
    GATTCAGCTAGTAAATCAACATCATTAAGCGATTCTATTTCAAACTCTAGCAGTACTGAA
    AAATCCGAAAGTCTATCAACAAGTACATCTGATTCATTGCGTACATCAACATCACTCAGT
    GACTCATTAAGTATGAGTACATCAGGAAGCTTGTCTAAGTCACAAAGCTTATCAACGAGT
    ATATCAGGGTCGTCTAGTACATCAGCATCATTAAGTGACAGTACATCGAATGCAATTAGT
    ACATCAACATCATTGAGCGAGTCAGCTAGCACCTCGGACTCTATCAGTATTTCAAATAGC
    ATAGCCAACTCTCAAAGTGCGTCAACAAGCAAATCAGATTCACAAAGTACATCAATATCA
    TTAAGTACAAGTGATTCAAAATCGATGAGTACATCAGAATCATTGAGCGATTCGACGAGC
    ACAAGTGGTTCTGTTTCTGGATCACTAAGCATAGCAGCATCACAAAGTGTCTCAACAAGT
    ACATCAGACTCGATGAGTACTTCAGAGATAGTAAGTGACTCTATCAGTACAAGTGGGTCA
    TTATCTGCATCAGACAGTAAATCAATGTCCGTAAGTAGTTCAATGAGCACGTCTCAGTCA
    GGTAGTACATCAGAATCATTAAGTGATTCACAAAGTACATCTGATTCTGATAGTAAGTCA
    TTATCACAAAGTACTAGTCAATCAGGTTCAACAAGTACATCAACGTCGACAAGTGCTTCA
    GTACGTACTTCGGAATCACAAAGTACGTCTGGTTCAATGAGTGCAAGTCAATCCGATTCA
    ATGAGCATATCAACGTCGTTTAGTGATTCAACGAGTGATAGCAAATCAGCATCAACTGCA
    TCAAGTGAATCAATATCACAAAGTGCTTCTACGAGCACATCTGGTTCGGTAAGTACTTCG
    ACATCGTTAAGTACAAGTAATTCAGAACGTACATCAACATCTATGAGTGATTCCACAAGC
    TTAAGTACATCAGAGTCTGATTCAATAAGTGAATCAACGTCAACGAGCGACTCTATAAGT
    GAAGCAATATCTGCTTCAGAGAGCACGTTTATATCATTAAGTGAATCAAATAGTACTAGC
    GATTCAGAATCACAAAGTGCATCTGCCTTTTTAAGTGAATCATTAAGTGAAAGTACGTCT
    GAATCAACATCAGAGTCAGTGAGTAGTTCGACAAGTGAGAGTACGTCATTATCAGACAGT
    ACATCAGAATCTGGTAGCACATCAACATCATTAAGTAATTCAACAAGTGGTAGTACGTCC
    ATTTCAACATCGACAAGTATCAGTGAATCAACGTCAACGTTTAAGAGCGAGAGTGTTTCA
    ACATCACTGAGTATGTCAACGAGTACAAGTTTGTCTGACTCTACAAGTTTGTCAACATCA
    TTAAGTGATTCCACAAGTGATAGTAAGTCTGATTCATTAAGTACATCAATGTCGACAAGT
    GATTCAATCAGTACAAGTAAATCTGATTCCATTAGTACATCCACATCATTAAGTGGTTCT
    ACAAGTGAAAGTAAATCCGATTCAACATCAATGAGCATAAGTATGTCTCAATCAACATCA
    GGAAGTACAAGTACGTCAACGAGTACAAGTTTGTCTGACTCAACGAGTACATCATTGTCA
    CTAAGTGCCTCAATGAATCAAAGCGGAGTAGACTCAAACTCAGCAAGCCAAAGTGCCTCA
    AACTCAACAAGTACAAGCACGAGCGAATCCGATTCACAAAGCACATCATCATATACAAGT
    CAGTCAACAAGCCAAAGTGAATCCACATCGACATCAACGTCACTAAGCGATTCAACAAGT
    ATATCTAAAAGTACGAGTCAATCAGGTTCGGTAAGCACATCAGCGTCATTAAGTGGTTCA
    GAGAGTGAATCTGATTCACAAAGTATCTCAACAAGTGCAAGTGAGTCAACATCAGAAAGT
    GCGTCAACATCACTCAGTGACTCAACAAGTACAAGTAACTCAGGATCAGCAAGTACGTCA
    ACATCGCTCAGTAACTCAGCAAGCGCAAGTGAATCCGATTTGTCGTCAACATCTTTAAGT
    GATTCAACATCTGCGTCAATGCAAAGCAGTGAATCCGATTCACAAAGCACATCAGCATCA
    TTAAGTGATTCGCTAAGTACATCAACTTCAAACCGCATGTCGACCATTGCAAGTTTATCT
    ACATCGGTAAGTACATCAGAGTCTGGCTCAACATCAGAAAGTACAAGTGAATCCGATTCA
    ACATCAACATCATTAAGCGATTCACAAAGCACATCAAGAAGTACAAGTGCATCAGGATCA
    GCAAGTACATCAACATCAACAAGTGACTCTCGTAGTACATCAGCTTCAACTAGTACTTCG
    ATGCGTACAAGTACTAGTGATTCACAAAGTATGTCGCTTTCGACAAGTACATCAACAAGT
    ATGAGTGATTCAACGTCATTATCTGATAGTGTTAGTGATTCAACATCAGACTCAACAAGT
    GCGAGTACATCTGGTTCGATGAGTGTGTCTATATCGTTAAGTGATTCGACAAGTACATCA
    ACATCGGCTAGTGAAGTAATGAGCGCAAGCATATCTGATTCACAAAGTATGTCAGAATCT
    GTAAATGATTCAGAAAGTGTAAGTGAATCTAATTCTGAAAGTGACTCTAAATCGATGAGT
    GGCTCAACAAGTGTCAGTGATTCTGGCTCATTGAGCGTCTCAACGTCATTAAGAAAATCA
    GAAAGTGTAAGCGAGTCAAGTTCATTGAGTTGCTCACAATCGATGAGCGATTCAGTAAGC
    ACAAGCGATTCGTCATCATTAAGTGTATCGACGTCACTAAGAAGTTCAGAAAGCGTGAGT
    GAATCTGATTCATTAAGTGATTCAAAATCAACAAGTGGTTCGACTTCAACAAGTACATCT
    GGTTCATTGAGTACCTCAACATCATTAAGTGGTTCAGAAAGCGTAAGCGAGTCTACCTCG
    CTAAGTGATTCAATATCAATGAGTGATTCTACTAGTACAAGTGACTCCGACTCATTAAGT
    GGATCAATATCTTTAAGTGGTTCCACAAGTCTTAGCACTTCGGATTCATTAAGTGATTCA
    AAATCATTGAGTAGCTCGCAAAGTATGAGTGGATCAGAATCAACGTCAACAAGTGTGAGC
    GATTCGCAGTCAAGCTCAACAAGTAATAGTCAATTTGACTCTATGAGCATCAGTGCATCA
    GAAAGCGACTCAATGTCTACAAGTGATTCGTCTAGCATCAGTGGATCAAATTCAACGAGT
    ACATCACTTTCAACATCTGACTCAATGAGCGGAAGCGTATCAGTTTCAACATCGACAAGT
    TTAAGTGACTCAATATCAGGTTCAACAAGTGTAAGTGACTCGAGCTCAACAAGCACATCT
    ACATCATTAAGTGATTCAATGTCACAAAGCCAGTCAACAAGTACAAGTGCATCTGGTTCC
    TTAAGTACATCGATATCAACATCAATGTCAATGAGTGCTAGTACATCGTCATCACAAAGC
    ACATCGGTGTCGACATCATTATCAACATCAGACAGTATCAGTGATTCTACTTCAATAAGT
    ATCAGTGGTTCACAAAGTACAGTAGAATCAGAATCTACAAGTGATTCAACTTCTATCAGT
    GACTCAGAATCATTGAGTACATCAGATTCAGACTCGACATCGACAAGTACATCGGACTCA
    ACAAGTGGTTCAACTTCAACAAGCATATCTGAATCATTAAGTACGTCTGGTTCAGGTTCA
    ACGAGCGTATCTGACTCAACATCAATGAGTGAATCTAATTCATCGAGTGTTTCAATGTCA
    CAAGACAAATCCGACTCAACATCAATTAGTGACTCAGAATCAGTGTCAACAAGCACATCA
    ACGTCATTGAGCACATCCGATTCGACAAGCACATCCGAATCACTGAGTACATCTATGTCT
    GGTTCACAAAGCATTTCTGACTCAACATCAACAAGTATGTCCGGCTCAACAAGTACATCT
    GAATCTAACTCAATGCATCCGTCAGACTCAATGAGTATGCATCATACTCACAGCACGAGC
    ACATCTCGCTTATCAAGTGAAGCAACAACGAGCACGAGTGAATCTCAGTCTACATTAAGT
    GCAACATCTGAAGTGACTAAACATAATGGCACACCAGCACAAAGTGAAAAAAGATTGCCA
    GATACAGGTGACTCAATAAAACAAAATGGATTACTAGGTGGCGTTATGACATTATTAGTT
    GGTTTAGGTTTAATGAAGAGAAAGAAAAAGAAAGATGAAAATGATCAAGATGATTCTCAA
    GCATAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS14015 [old locus tag: SACOL2676 ]
  • symbol: SACOL_RS14015
  • description: serine-rich repeat glycoprotein adhesin SasA
  • length: 2261
  • theoretical pI: 3.9827
  • theoretical MW: 227019
  • GRAVY: -0.53441

⊟Function[edit | edit source]

  • TIGRFAM:
    KxYKxGKxW signal peptide (TIGR03715; HMM-score: 28.4)
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 24.4)
  • TheSEED: see SACOL2676
  • PFAM:
    no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 34.2)
    and 1 more
    E-set (CL0159) HYR; HYR domain (PF02494; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01278
    • TAT(Tat/SPI): 0.001466
    • LIPO(Sec/SPII): 0.001104
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGLPFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNTQSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQQEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVNLNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNATYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGFAFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAFGAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQTWTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGKDIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQSVTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSATNSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPISPIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKTTTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKSTSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNSSSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDSTSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTSESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMSVSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQSTSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTSNSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSESQSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSISTSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTSKSDSISTSTSLSGSTSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQSESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTSLSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNSQFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGSTSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTSLSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGSTSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTSLSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTSTSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTLLVGLGLMKRKKKKDENDQDDSQA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL2676
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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