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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00060
  • pan locus tag?: SAUPAN000899000
  • symbol: SAOUHSC_00060
  • pan gene symbol?: nptA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00060
  • symbol: SAOUHSC_00060
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 64265..65926
  • length: 1662
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCGGTTACAGAAGTCATTTTCTCCTTTTTAGGTGGTTTAGGTATTTTCCTTTACGGC
    TTAAAAATCATGGGAGACGGGCTTCAAGCATCAGCAGGAGACAGGCTACGAGATATTTTA
    AACAAATTTACATCAAATCCAGTATTAGGTGTTATTGCAGGTATCGTTGTAACTATTTTA
    ATACAAAGTAGTTCAGGTACGACAGTTATCACAATCGGACTGGTAACAGCTGGATTTATG
    ACATTGAAACAAGCCATTGGAGTGATAATGGGTGCTAATATCGGAACAACGGTAACTGCA
    TTTATTATCGGTATAGATTTAGGCGAATATGCAATGCCAATTTTAGCATTAGGTGCATTC
    TTAATCTTTTTCTTTAAACGCTCTAAAATCAATAACATTGGCCGCATACTATTCGGTTTC
    GGTTCACTATTCTTCGGTCTAGAATTTATGGGTGATGCCGTTAAACCTTTAGCATCATTA
    GATGGATTTAAGCAATTAATGCTTGATATGTCTACAAATCCAATACTCGCTGTCATTGTC
    GGCGCAGGGTTAACAGCACTAGTTCAAAGTTCAAGTGCGACGATTGGTATTTTACAAGAA
    TTTTATCAACAAGATTTAATTAGCTTAAACGCAGCAATCCCTGTGTTACTAGGCGATAAC
    ATTGGTACCACGATTACAGCTATCTTAGCTAGTTTAGCCGGCTCAATCGCTGCAAAACGT
    GCGGCGCTTGTACACGTCATCTTTAACTTAATCGGGGTAATTATCTTCACAATTTTCTTG
    CCAGTTGTGATTCATTTGATTAGTTTGTTACAAGATTTATGGCACTTAAAACCAGCGATG
    ACGATTGCAGTATCACATGGTATCTTCAACATAACAAATACTTTGATTCAATTACCATTT
    GTAGCAGGTTTAGCATGGATTGTTACAAAGCTTGTCCCAGGTAAAGATATTGCTGATGAC
    TATAAACCTCAGCACTTAAACAAAGATCTTGTTTATCACGCACCTGGTGTTGCATTACAA
    GAAACTCAAAAAGAATTACAAAATGTGGGTCAGATTGTCTTATCAATGTTTGAAGACATT
    CGCGAAATTACAAAAGACGATAAAAAATTGATCAAAAAGCTTGAACAAAAGCATCAAGCT
    GTTGAAACAATCAATGATAGCATTCGAAATTATTTAGTTAGAATTTCTACAAAAGCCATT
    ACGAAGGCAGACGTTGAGCGTTTAGCAGTTATGTTTGATGTCAATCGCTCTATTTTAAAA
    GTAGCAGAGCTAACAGAAGAGTATGTCGCTCAATTAAAACGCCAACATGATGAAGATATT
    CGCATTACAGAAGATGCACAACGCGGTATGGATAAATTATTCAACCATGTTGCTGAGTCA
    TTTGATAAAGCCATCGACATGTTAGATGTTTATGACAAAACGAAAAAAGATGAAATTGTA
    GAACGTAGTAGAGAATCATTTAATATTGAACATAAACTACGCAAAGGTCATATTAAACGC
    CTTAATCGTGGTGAATGTACAACAAAAGGCGGATTACTATATATCGATATGATTGGTGTT
    CTTGAACGTATCGGTTATCATTCACGAAATGTTTCTGAAGCACTTGTTGGCCTTAACGAT
    GATGTACCTACAGATGAAGAAATTGCAACAACTGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00060
  • symbol: SAOUHSC_00060
  • description: hypothetical protein
  • length: 553
  • theoretical pI: 6.38787
  • theoretical MW: 60647.5
  • GRAVY: 0.333454

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds Na/Pi-cotransporter II-related protein (TIGR00704; HMM-score: 250.1)
    and 3 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds sodium-dependent inorganic phosphate (Pi) transporter (TIGR01013; HMM-score: 121.5)
    Metabolism Transport and binding proteins Anions phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 34.8)
    Signal transduction Regulatory functions Other phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 34.8)
  • TheSEED  :
    • Na/Pi cotransporter II-related protein
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Na/Pi cotransporter II-related protein
  • PFAM:
    no clan defined Na_Pi_cotrans; Na+/Pi-cotransporter (PF02690; HMM-score: 234.3)
    and 8 more
    PhoU (CL0297) PhoU; PhoU domain (PF01895; HMM-score: 49.8)
    no clan defined ApoO; Apolipoprotein O (PF09769; HMM-score: 13.8)
    Mis12; Mis12 protein (PF05859; HMM-score: 13.4)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 11.8)
    no clan defined IBP39; Initiator binding protein 39 kDa (PF11422; HMM-score: 11.7)
    MutS_III; MutS domain III (PF05192; HMM-score: 11.1)
    KxDL; Uncharacterized conserved protein (PF10241; HMM-score: 8.2)
    Med30; Mediator complex subunit 30 (PF11315; HMM-score: 7.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.07588
    • TAT(Tat/SPI): 0.006252
    • LIPO(Sec/SPII): 0.006966
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFTSNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFIIGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASLDGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLGDNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHLKPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHAPGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYLVRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGMDKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTTKGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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