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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00779
  • pan locus tag?: SAUPAN002675000
  • symbol: SAOUHSC_00779
  • pan gene symbol?: uvrB2
  • synonym:
  • product: excinuclease ABC subunit B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00779
  • symbol: SAOUHSC_00779
  • product: excinuclease ABC subunit B
  • replicon: chromosome
  • strand: +
  • coordinates: 758680..759654
  • length: 975
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTGATGAATCACATGTGACATTACCGCAAGTTCGAGGCATGTATAACGGAGACAGA
    GCGCGTAAACAAGTTTTGGTGGATCATGGGTTTAGATTACCGAGTGCATTAGATAACCGT
    CCACTTAAATTTGAAGAATTTGAAGAAAAGACAAAACAACTTGTGTATGTATCTGCAACG
    CCTGGACCATACGAAATTGAACATACGGATAAGATGGTTGAACAAATTATTCGTCCTACT
    GGTTTACTGGATCCTAAGATTGAGGTTAGACCTACTGAAAATCAAATTGACGATTTATTA
    AGTGAAATTCAAACAAGAGTTGAGCGTAATGAACGCGTACTTGTTACAACGCTCACTAAA
    AAGATGAGTGAAGATTTAACCACATACATGAAAGAAGCGGGTATTAAAGTTAATTATCTG
    CATTCAGAAATCAAGACATTAGAACGAATTGAAATAATTAGAGACTTACGAATGGGTACA
    TATGATGTTATCGTAGGTATTAATTTATTAAGAGAGGGTATTGATATACCAGAAGTTTCT
    CTAGTTGTCATATTAGATGCAGATAAAGAAGGGTTTTTACGTTCTAACCGCTCATTAATT
    CAAACAATAGGTAGAGCTGCGCGTAACGATAAAGGTGAAGTCATTATGTATGCCGATAAA
    ATGACTGATTCGATGAAGTATGCAATTGATGAGACACAACGTCGTCGAGAAATACAGATG
    AAACATAATGAAAAACATGGTATTACACCTAAAACAATTAATAAAAAAATACATGATTTA
    ATTAGTGCTACTGTTGAAAATGACGAAAATAATGACAAAGCACAAACTGTGATACCTAAG
    AAGATGACGAAAAAAGAACGTCAAAAGACAATCGACAATATAGAAAAAGAAATGAAACAA
    GCAGCGAAAGATTTAGATTTCGAGAAAGCTACAGAATTAAGAGATATGTTATTTGAATTA
    AAAGCAGAAGGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00779
  • symbol: SAOUHSC_00779
  • description: excinuclease ABC subunit B
  • length: 324
  • theoretical pI: 6.21157
  • theoretical MW: 37476.8
  • GRAVY: -0.672531

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 533.6)
    and 10 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 33.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 31.1)
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 31)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 30.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 29.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 20.8)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 18.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit C (TIGR00194; EC 3.1.25.-; HMM-score: 17)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 15.3)
    CRISPR type I-A/APERN-associated protein Csa5 (TIGR01878; HMM-score: 5.7)
  • TheSEED  :
    • Excinuclease ABC subunit B
    DNA Metabolism DNA repair DNA repair, UvrABC system  Excinuclease ABC subunit B
  • PFAM:
    P-loop_NTPase (CL0023) UvrB; Ultra-violet resistance protein B (PF12344; HMM-score: 85.8)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 75.6)
    and 2 more
    no clan defined UVR; UvrB/uvrC motif (PF02151; HMM-score: 36.6)
    PDDEXK (CL0236) Sen15; Sen15 protein (PF09631; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003806
    • TAT(Tat/SPI): 0.001246
    • LIPO(Sec/SPII): 0.001384
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLVYVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERVLVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLREGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAIDETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKERQKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LexA* (repression) regulon
    LexA*(TF)important in SOS response; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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