From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00828
  • pan locus tag?: SAUPAN002812000
  • symbol: SAOUHSC_00828
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00828
  • symbol: SAOUHSC_00828
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 805315..805932
  • length: 618
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTAACCGCAATTATTCATGGATTTATCTTAGCGATTGGTCTAATTTTACCTTTAGGT
    GCACAAAATGTATTTATTTTTAACCAAGGAGCTAATCAACCAAAATATAGATATGTATTG
    CCTGCAATAATTACAGCCGGGTTGTCAGACAGCTTACTTATTATTATTGCAGTGGTAGGA
    GTATCTATCATTATTATGTCTTTACCTGTACTTCAAGCAATTATTTATATAGTTGGTTTA
    ATTTTCTTGATGTATATGGCTTGGACCATTTGGCATGATAAACCCTCAACAGATGGAGAA
    ACTCAAATTATGTCTCCAATGAAACAAGTAAGTTTTGCTTTATCAGTTTCATTACTCAAT
    CCACATGCTATTTTAGATACAATTGGAGTAATTGGTAGTAGTGCTGCATTATATAGTGGC
    AGCAATAAAATTGCGTTTACAATCGCTTGTATTAGCGTTTCATGGTTATGGTTCTTTTTA
    CTCGCAATTTTAGGAAAAATGGTTGGATCAATTGATAAAACCGGAAAATTATTAACAATA
    ATCAATAAAATATCAAGCATAATAATTATTATTGTTGCTCTGATGATATTACAAAAACTT
    ATTCAATTATTATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    618

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00828
  • symbol: SAOUHSC_00828
  • description: hypothetical protein
  • length: 205
  • theoretical pI: 9.71156
  • theoretical MW: 22211.9
  • GRAVY: 1.18488

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-lysine exporter (TIGR00948; HMM-score: 154.3)
    and 1 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines homoserine/Threonine efflux protein (TIGR00949; HMM-score: 35.3)
  • TheSEED  :
    • Transporter, LysE family
  • PFAM:
    LysE (CL0292) LysE; LysE type translocator (PF01810; HMM-score: 94.9)
    and 1 more
    no clan defined CAAD; CAAD domains of cyanobacterial aminoacyl-tRNA synthetase (PF14159; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.038834
    • TAT(Tat/SPI): 0.001598
    • LIPO(Sec/SPII): 0.07802
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAIITAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQIMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFLLAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_00828&oldid=99491"