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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00973
  • pan locus tag?: SAUPAN003234000
  • symbol: SAOUHSC_00973
  • pan gene symbol?: tarM
  • synonym:
  • product:

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00973
  • symbol: SAOUHSC_00973
  • product:
  • replicon: chromosome
  • strand: +
  • coordinates: 947205..948687
  • length: 1482
  • essential: no DEG
  • comment: The sequence of SAOUHSC_00973 was corrected based on the resequencing performed by Berscheid et al., 2012 [1].

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAAAAAATATTTATGATGGTACATGAGTTAGATGTGAATAAAGGTGGTATGACCTCT
    TCGATGTTCAATAGAAGTAAAGAGTTTTATGATGCGGACATACCTGCTGATATTGTTACT
    TTCGATTACAAAGGAAACTATGATGAAATTATTAAAGCTTTGAAAAAACAAGGTAAAATG
    GATCGAAGAACGAAAATGTATAATGTATTTGAGTATTTTAAACAAATTTCAAATAATAAA
    CATTTTAAGTCTAATAAATTGTTATATAAACATATTTCAGAAAGACTAAAAAATACGATT
    GAAATTGAAGAGAGTAAAGGTATTTCAAGATATTTTGATATAACGACTGGTACATATATT
    GCCTACATTAGAAAAAGTAAATCTGAAAAAGTGATTGATTTCTTTAAAGATAATAAACGA
    ATTGAACGGTTTAGTTTTATAGATAATAAAGTGCATATGAAGGAAACATTTAATGTAGAT
    AATAAAGTTTGTTATCAAGTATTTTATGATGAAAAGGGATACCCATATATTTCAAGGAAT
    ATTAATGCTAATAATGGTGCTGTAGGTAAAACTTATGTGTTAGTTAATAAAAAAGAATTT
    AAAAACAATTTAGCACTGTGTGTTTACTATTTAGAAAAACTAATAAAAGATTCTAAAGAT
    AGTATTATGATTTGTGATGGACCAGGGAGTTTTCCAAAAATGTTTAATACAAATCATAAA
    AATGCTCAGAAATATGGCGTTATTCATGTTAATCATCATGAAAATTTCGATGATACGGGT
    GCATTTAAAAAAAGTGAGAAATATATTATTGAGAATGCGAATAAAATTAACGGTGTAATT
    GTATTAACAGAGGCACAAAGATTAGATATTCTTAATCAATTTGATGTAGAAAATATTTTC
    ACTATTAGCAATTTTGTTAAGATACATAATGCTCCAAAACATTTTCAAACTGAAAAAATC
    GTAGGTCATATTTCTAGAATGGTACCAACGAAGCGAATTGATTTGCTTATTGAAGTGGCT
    GAGTTAGTCGTAAAAAAAGATAATGCTGTTAAATTTCATATATATGGAGAAGGATCTGTC
    AAAGATAAAATAGCTAAAATGATTGAAGATAAAAATTTAGAAAGAAATGTTTTTCTTAAA
    GGATATACAACAACTCCACAAAAATGCTTGGAAGATTTTAAATTAGTCGTTTCTACATCT
    CAATATGAAGGTCAAGGGTTAAGTATGATAGAAGCAATGATTTCTAAAAGGCCTGTTGTT
    GCCTTTGACATCAAATACGGACCAAGTGATTTTATAGAAGATAATAAAAATGGTTATTTA
    ATAGAAAACCATAATATTAACGACATGGCTGATAAAATACTTCAGCTTGTTAATAATGAT
    GTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACG
    GAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1482

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00973
  • symbol: SAOUHSC_00973
  • description:
  • length: 493
  • theoretical pI: 9.34538
  • theoretical MW: 57274.4
  • GRAVY: -0.49858

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 117)
    and 15 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 89.2)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 84.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 35.2)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 32.7)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 31.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 29.3)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 29)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 27.7)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 25.5)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 24.8)
    sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 19.6)
    Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 16.6)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03087; HMM-score: 16.4)
    putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 14.8)
    RNA polymerase sigma factor, SigM family (TIGR02950; HMM-score: 12.8)
  • TheSEED  :
    • Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
    Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 112.3)
    and 4 more
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 88.5)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 32.5)
    no clan defined DUF4116; Domain of unknown function (DUF4116) (PF13475; HMM-score: 13.9)
    DUF3695; Protein of unknown function (DUF3695) (PF12494; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010241
    • TAT(Tat/SPI): 0.000295
    • LIPO(Sec/SPII): 0.001551
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt: Q2FZM7 UniProt
  • STRING: 93061.SAOUHSC_00973 STRING

⊟Protein sequence[edit | edit source]

  • MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYKGNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEIEESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMADKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
    Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
    Int J Med Microbiol: 2012, 302(2);84-7
    [PubMed:22417616] [WorldCat.org] [DOI] (I p)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Solmaz Sobhanifar, Liam James Worrall, Robert J Gruninger, Gregory A Wasney, Markus Blaukopf, Lars Baumann, Emilie Lameignere, Matthew Solomonson, Eric D Brown, Stephen G Withers, Natalie C J Strynadka
Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid α-glycosyltransferase.
Proc Natl Acad Sci U S A: 2015, 112(6);E576-85
[PubMed:25624472] [WorldCat.org] [DOI] (I p)

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