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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01102
  • pan locus tag?: SAUPAN003385000
  • symbol: uvrC
  • pan gene symbol?: uvrC
  • synonym:
  • product: excinuclease ABC subunit C

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01102
  • symbol: uvrC
  • product: excinuclease ABC subunit C
  • replicon: chromosome
  • strand: +
  • coordinates: 1062565..1064346
  • length: 1782
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    TTGGAAGACTATAAGCAACGAATTAAAAATAAATTAAATGTCGTACCTATGGAACCAGGA
    TGCTATTTAATGAAAGATCGTAATGATCAAGTGATATATGTTGGCAAAGCTAAAAAGCTA
    AGAAATCGATTGCGATCATATTTTACGGGTGCTCATGATGCTAAAACAACGAGACTGGTT
    GGTGAAATACGTCGCTTTGAGTTTATTGTCACGTCAAGTGAAACAGAATCACTTTTACTG
    GAATTGAATCTGATTAAACAATATCAACCAAGATATAATATATTATTAAAGGATGATAAA
    AGTTATCCATTTATTAAAATTACGAAGGAGAAATATCCTAGACTACTAGTGACGAGAACT
    GTAAAACAAGGTACTGGCAAATATTTCGGACCGTATCCGAATGCATATTCTGCTCAAGAA
    ACTAAAAAGTTATTAGACAGAATATATCCATATCGCAAATGTGATAAGATGCCAGATAAA
    TTATGTCTTTATTACCATATTGGACAATGTTTAGGACCATGTGTATATGACGTTGATTTG
    AGTAAATACGCACAAATGACGAAGGAAATTACTGATTTTCTGAATGGGGAAGACAAAACA
    ATTTTAAAAAGTTTAGAAGAGCGAATGTTAACTGCAAGTGAATCACTTGATTTTGAACGG
    GCTAAAGAATATAGAGATTTAATTCAACATATTCAAAATCTGACAAACAAACAAAAAATT
    ATGTCATCAGATAAAACGATTCGTGATGTCTTTGGTTATAGTGTTGATAAAGGATGGATG
    TGTATCCAAGTTTTCTTTATACGACAAGGTAATATGATAAAGCGAGATACAACGATGATT
    CCATTACAGCAAACAGAAGAAGAAGAATTTTATACATTTATTGGACAATTTTATAGCTTA
    AACCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATT
    CAATCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTT
    GACCTAGCTGCACATAACGCTAAAGTATCCTTAAATAATAAATTTGAATTAATATCACGT
    GATGAGTCCAGAACGATTAAAGCTATTGAAGAACTTGGAACACAAATGGGAATTCAAACA
    CCAATTAGAATTGAAGCATTCGATAATTCTAATATTCAAGGTGTGGATCCAGTGTCAGCA
    ATGGTTACATTTGTCGACGGTAAACCAGATAAGAAAAATTATAGAAAGTATAAAATCAAA
    ACGGTTAAAGGTCCAGATGATTACAAATCAATGAGAGAAGTAGTAAGACGACGATATTCT
    CGCGTTTTAAACGAAGGATTACCATTACCTGATTTAATAATAGTAGATGGTGGTAAAGGA
    CATATGAACGGGGTTATTGATGTGCTACAAAACGAATTAGGTCTTGATATCCCTGTTGCA
    GGTTTGCAGAAAAATGATAAACACCAAACATCTGAATTATTATATGGCGCTAGTGCAGAA
    ATTGTACCACTGAAGAAAAATAGCCAGGCATTTTATTTGTTGCACCGTATCCAAGATGAG
    GTTCACAGATTCGCAATCACATTTCATAGACAAACACGTCAAAAGACAGGCTTGAAATCA
    ATACTTGATGATATAGATGGTATCGGTAACAAACGTAAAACATTATTATTGCGTTCATTC
    GGTTCAATCAAGAAAATGAAGGAAGCTACACTTGAAGATTTTAAAAATATAGGTATTCCT
    GAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1782

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01102
  • symbol: UvrC
  • description: excinuclease ABC subunit C
  • length: 593
  • theoretical pI: 9.78595
  • theoretical MW: 68670.9
  • GRAVY: -0.577234

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit C (TIGR00194; EC 3.1.25.-; HMM-score: 660.4)
    and 1 more
    group I intron endonuclease (TIGR01453; HMM-score: 13.5)
  • TheSEED  :
    • Excinuclease ABC subunit C
    DNA Metabolism DNA repair DNA repair, UvrABC system  Excinuclease ABC subunit C
  • PFAM:
    Endonuclease (CL0189) UvrC_HhH_N; UvrC Helix-hairpin-helix N-terminal (PF08459; HMM-score: 179.3)
    and 4 more
    GIY-YIG (CL0418) GIY-YIG; GIY-YIG catalytic domain (PF01541; HMM-score: 44.9)
    no clan defined UVR; UvrB/uvrC motif (PF02151; HMM-score: 40)
    HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 18.4)
    no clan defined DUF3936; Protein of unknown function (DUF3936) (PF13072; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00368
    • TAT(Tat/SPI): 0.000393
    • LIPO(Sec/SPII): 0.001302
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRLRSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSYPFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDKLCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDFERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRDTTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARGPKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNIQGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLPDLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNSQAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKMKEATLEDFKNIGIPENVAKNLHEQLHK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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