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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01257
- pan locus tag?: SAUPAN003582000
- symbol: SAOUHSC_01257
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_01257
- symbol: SAOUHSC_01257
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 1212021..1212725
- length: 705
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3919988 NCBI
- RefSeq: YP_499790 NCBI
- BioCyc: G1I0R-1175 BioCyc
- MicrobesOnline: 1289704 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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661ATGAAAGCATTAGTATTAGGTGGTTCTGGTTCAATTGGTTCTGAGATAGTCAAACAATTA
TTAACTGATGGATTTGAAGTTTATGTGCAATATTATCGTACTGATATAAATGAATTAACT
AGCAAATTTAATGATGATAAAGTTCGTTTTATACAAGCGGATTTATCTCAAACAATTGAT
ATTGACAAAACATTTGGTGACATTAAATCATTAGACTGTTTAATATATGCAAGTGGTCAG
TCTTTATATGGTGTTTTACAAGATATGAAAGACCATGATATTGATGCATGTTATCAGTTA
AATGTCTTGCAATTAATTCGATTATGTAGATATTTCGTTGATGTTTTACGTCAAAGTGCC
AATGGAAGAATTATTGTAATTTCATCAATTTGGGGTGAGACAGGAGCTAGTATGGAAACT
ATTTATTCGGCGATGAAAAGTGCACAATTAGGTTTCGTTAAGGCGCTTAGTCAAGAGCTT
GCACTAACATCAGTGACAGTAAATGCTATCGCACCTGGATTTGTAGCCGGTAATATGGCA
AGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTG
GTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGT
GTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01257
- symbol: SAOUHSC_01257
- description: hypothetical protein
- length: 234
- theoretical pI: 4.61995
- theoretical MW: 25924.4
- GRAVY: 0.0589744
⊟Function[edit | edit source]
- TIGRFAM: acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 142.7)Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 140.9)and 18 more3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 97.6)Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 85.3)Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 85.1)Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 81.5)Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 77.4)2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 73.4)Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 67.7)2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 54)pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 47.2)cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 46.3)rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 38)Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 27.5)sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 26.6)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 16)hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 15.9)ergot alkaloid biosynthesis protein, AFUA_2G17970 family (TIGR03649; HMM-score: 14.8)UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 14.3)Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 13.4)
- TheSEED :
- 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)
- PFAM: NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 124)adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 118)and 9 moreEpimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 31.1)NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 30.8)KR; KR domain (PF08659; HMM-score: 27.9)3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 21.5)NmrA; NmrA-like family (PF05368; HMM-score: 21.5)GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 21.5)Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 13.6)RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 12.6)HTH (CL0123) RepL; Firmicute plasmid replication protein (RepL) (PF05732; HMM-score: 11.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007583
- TAT(Tat/SPI): 0.000124
- LIPO(Sec/SPII): 0.001252
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_01253 > SAOUHSC_01254 > SAOUHSC_01255 > SAOUHSC_01256 > SAOUHSC_01257 > SAOUHSC_01258 > SAOUHSC_01259 > SAOUHSC_01260predicted SigA promoter [3] : S521 > SAOUHSC_01252 > S522 > SAOUHSC_01253 > SAOUHSC_01254 > SAOUHSC_01255 > SAOUHSC_01256 > SAOUHSC_01257 > S523 > SAOUHSC_01258 > SAOUHSC_01259 > SAOUHSC_01260
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)