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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01370
  • pan locus tag?: SAUPAN003766000
  • symbol: SAOUHSC_01370
  • pan gene symbol?: trpF
  • synonym:
  • product: N-(5'-phosphoribosyl)anthranilate isomerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01370
  • symbol: SAOUHSC_01370
  • product: N-(5'-phosphoribosyl)anthranilate isomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 1314194..1314826
  • length: 633
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAATTGAAATTTTGTGGCTTTACATCAATAAAGGATGTTACAGCGGCCAGTCAATTA
    CCTATTGATGCGATAGGTTTCATCCATTATGAAAAAAGTAAAAGGCATCAAACAATTACC
    CAAATAAAAAAGTTAGCGTCTGCTGTTCCAAATCATATCGATAAAGTATGTGTCATGGTA
    AATCCTGATTTAACAACAATTGAACACGTATTAAGCAATACGTCAATTAACACAATACAG
    TTACACGGCACAGAATCTATTGATTTTATACAGGAAATTAAAAAGAAATATTCAAGCATT
    AAAATCACTAAAGCTTTAGCTGCAGATGAAAACATAATCCAAAACATAAATAAATATAAA
    GGGTTCGTAGATTTATTTATTATCGACACACCCTCAGTGTCGTATGGTGGTACCGGTCAA
    ACATATGACTGGACTATTTTGAAGCACATAAAAGACATACCTTATTTGATAGCAGGAGGC
    ATTAACTCTGAAAATATTCAAACAGTTAATCAACTTAAATTATCACATCAAGGTTATGAT
    CTTGCATCAGGTATAGAAGTAAATGGGCGAAAAGATATAGAAAAAATGACAGCAATTGTA
    AATATTGTGAAAGGAGATAGAGACAATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01370
  • symbol: SAOUHSC_01370
  • description: N-(5'-phosphoribosyl)anthranilate isomerase
  • length: 210
  • theoretical pI: 8.22155
  • theoretical MW: 23388.7
  • GRAVY: -0.201429

⊟Function[edit | edit source]

  • reaction:
    EC 5.3.1.24?  ExPASy
    Phosphoribosylanthranilate isomerase N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
  • TIGRFAM:
    His-Xaa-Ser system putative quinone modification maturase (TIGR03981; HMM-score: 12.3)
  • TheSEED  :
    • Phosphoribosylanthranilate isomerase (EC 5.3.1.24)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Tryptophan synthesis  Phosphoribosylanthranilate isomerase (EC 5.3.1.24)
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Phosphoribosylanthranilate isomerase (EC 5.3.1.24)
  • PFAM:
    TIM_barrel (CL0036) PRAI; N-(5'phosphoribosyl)anthranilate (PRA) isomerase (PF00697; HMM-score: 130.8)
    and 3 more
    PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 14.6)
    no clan defined Mer2; Mer2 (PF09074; HMM-score: 12.9)
    DUF1995; Domain of unknown function (DUF1995) (PF09353; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011139
    • TAT(Tat/SPI): 0.000449
    • LIPO(Sec/SPII): 0.001166
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKKLASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKITKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGGINSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: T-box(Trp) (transcription antitermination) regulon, CodY* (repression) regulon
    T-box(Trp)(5' cis-acting region)important in Amino acid metabolism; transcription unit transferred from N315 data RegPrecise 
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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