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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01515
- pan locus tag?:
- symbol: SAOUHSC_01515
- pan gene symbol?: —
- synonym:
- product: petidoglycan hydrolase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_01515
- symbol: SAOUHSC_01515
- product: petidoglycan hydrolase
- replicon: chromosome
- strand: -
- coordinates: 1464415..1465869
- length: 1455
- essential: unknown
⊟Accession numbers[edit | edit source]
- Gene ID: 3919056 NCBI
- RefSeq: YP_500032 NCBI
- BioCyc: G1I0R-1406 BioCyc
- MicrobesOnline: 1289946 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1441ATGTTGATAACAAAAAACCAAGCAGAAAAATGGTTTGATAATTCATTAGGGAAGCAGTTC
AATCCTGATTTGTTTTATGGATTTCAGTGTTACGATTACGCAAATATGTTTTTTATGATA
GCAACAGGCGAAAGGTTACAAGGTTTATACGCTTATAATATTCCATTTGATAATAAAGCA
AGGATTGAAAAATACGGGCAAATAATTAAAAACTATGATAGCTTTTTACCGCAAAAGTTG
GACATTGTCGTTTTCCCGTCAAAGTATGGTGGCGGAGCTGGACATGTTGAAATTGTTGAG
AGCGCTAATCTAAACACTTTCACATCGTTTGGCCAAAATTGGAATGGTAAAGGTTGGACA
AATGGCGTTGCGCAACCTGGTTGGGGTCCCGAAACCGTTACAAGACATGTTCATTATTAC
GATGACCCAATGTATTTTATTAGATTAAATTTCCCAGATAAAGTAAGTGTTGGAGATAAA
GCTAAAAGCGTTATTAAGCAAGCAACTGCCAAAAAGCAAGCAGTAATTAAACCTAAAAAA
ATTATGCTTGTAGCCGGTCATGGTTATAACGATCCTGGAGCAGTCGGAAACGGAACAAAT
GAACGTGATTTTATCCGTAAATATATAACACCAAATATCGCTAAGTATTTAAGACATGCA
GGTCACGAAGTTGCATTATATGGTGGCTCAAGTCAATCACAAGATATGTATCAAGATACT
GCTTACGGTGTTAATGTAGGAAATAATAAAGATTATGGCTTATATTGGGTTAAATCACAT
GGGTATGACATTGTTCTAGAGATTCATTTAGACGCAGCAGGAGAAAGTGCAAGTGGTGGG
CATGTTATTATTTCAAGTCAATTCAATGCAGATACTATTGATAAAAGTATACAAGATGTT
ATTAAAAATAACTTAGGACAAATAAGAGGTGTAACACCTCGTAATGATTTACTAAACGTT
AATGTATCAGCAGAAATAAATATAAACTATCGTTTATCTGAATTAGGTTTTATTACTAAT
AAAAATGATATGGATTGGATTAAGAAAAACTATGACTTGTATTCTAAATTAATAGCCGGT
GCGATTCATGGTAAGCCTATAGGTGGTTTGGTAGCTGGTAATGTTAAAACATCAGCTAAA
AACCAAAAAAATCCACCAGTGCCAGCAGGTTATACACTTGATAAAAACAATGTACCGTAT
AAAAAAGAGACTGGTTATTACACAGTTGCCAATGTTAAAGGTAATAACGTAAGGGACGGC
TATTCAACTAATTCAAGAATTACAGGTGTATTACCTAATAACGCAACAATTAAATATGAC
GGCGCATATTGCATCAATGGCTATAGATGGATTACTTATATTGCTAATAGTGGACAACGT
CGTTATATAGCGACAGGAGAGGTAGACAAGGCAGGTAATAGAATAAGTAGTTTTGGTAAG
TTTAGCACGATTTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01515
- symbol: SAOUHSC_01515
- description: petidoglycan hydrolase
- length: 484
- theoretical pI: 9.59909
- theoretical MW: 53829.2
- GRAVY: -0.472934
⊟Function[edit | edit source]
- TIGRFAM: Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.4)Cellular processes Sporulation and germination N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.4)
- TheSEED :
- Phage endolysin CHAP endopeptidase
- Phage endolysin N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
- PFAM: Peptidase_MH (CL0035) Amidase_3; N-acetylmuramoyl-L-alanine amidase (PF01520; HMM-score: 137.1)and 3 morePeptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 36.4)SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 31.8)SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 16)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.009029
- TAT(Tat/SPI): 0.000337
- LIPO(Sec/SPII): 0.000871
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMIATGERLQGLYAYNIPFDNKARIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDDPMYFIRLNFPDKVSVGDKAKSVIKQATAKKQAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNNKDYGLYWVKSHGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSELGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNQKNPPVPAGYTLDKNNVPYKKETGYYTVANVKGNNVRDGYSTNSRITGVLPNNATIKYDGAYCINGYRWITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [1] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)