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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01606
  • pan locus tag?: SAUPAN004064000
  • symbol: SAOUHSC_01606
  • pan gene symbol?: —
  • synonym:
  • product: peptidase T

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01606
  • symbol: SAOUHSC_01606
  • product: peptidase T
  • replicon: chromosome
  • strand: -
  • coordinates: 1531153..1532286
  • length: 1134
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGATTAATGAGCAAAGATTACTTAATACGTTTTTTGAACTTGTACAAATCGATTCTGAG
    ACAGGGAATGAATCAACAATCCAACCTATTTTAAAAGAAAAATTCATAGCATTAGGGTTA
    GATGTTAAAGAAGATGAAGCGGCTAAACATCCTAAATTAGGTGCCAATAATCTAGTGTGT
    ACAATGAATAGTACTATCGAAGAAGGCGAAGTACCTAAATTATATTTGACGAGCCATATG
    GATACTGTTGTACCCGCAATTAATGTAAAGCCAATTGTAAAAGATGACGGCTACATATAC
    TCTGATGGTACGACTATTCTAGGTGCAGATGATAAAGCAGGATTATCAGCGATGCTTGAA
    GTTTTGCAAGTGATAAAGGAACAACAAATCCCACATGGACAAATTCAATTTGTGATTACT
    GTTGGAGAAGAATCAGGTTTAATTGGTGCTAAAGAATTAAATTCAGAGTTGTTGGACGCG
    GACTTTGGTTATGCTATTGATGCAAGTGCTGATGTCGGTACTACGGTTGTAGGTGCACCG
    ACGCAAATGTTAATTTCAGCTAAAATTTTTGGCAAAACGGCTCATGCAAGTACGCCAAAA
    GAGGGTGTTAGTGCTATTAATATTGCGGCGAAAGCAATTAGTCGAATGAAATTAGGACAA
    GTTGATGAAATAACTACTGCCAATATTGGTAAGTTTCATGGCGGTTCAGCTACGAATATT
    GTTGCAGATGAAGTCATTTTAGAAGCAGAGGCACGTTCTCACGATCCAGAAAGAATAAAA
    ACACAAGTCAAACATATGACTGATGTGTTTGAAACTACTGCATCAGAATTAGGTGGGAAA
    GCAGTAGTTACAGTGGAGCAAAGTTATCCAGGATTTAAAATTAATGATAATGAAGCGGTT
    GTTAAAATTGCACAAGAAAGTGCTCGTAATTTAGGATTATCTGCAAATACAATTATTTCT
    GGTGGCGGTTCAGACGGCAGTATTATTAATACATTTGGTATTCCTTCAGTCATTTTAGGA
    GTGGGTTATGAAAAAATCCATACAACAAATGAGAGAATGCCGATTAAATCATTGAACTTG
    CTTGCAAGTCAAGTGTTAGAAATAATCAAAATAGTTGCGCGACATTCGAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1134

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01606
  • symbol: SAOUHSC_01606
  • description: peptidase T
  • length: 377
  • theoretical pI: 4.79982
  • theoretical MW: 40259.6
  • GRAVY: -0.0161804

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.11.4?  ExPASy
    Tripeptide aminopeptidase Release of the N-terminal residue from a tripeptide
    EC 3.4.11.14?  ExPASy
    Cytosol alanyl aminopeptidase Release of an N-terminal amino acid, preferentially alanine, from a wide range of peptides, amides and arylamides
  • TIGRFAM:
    peptidase T-like protein (TIGR01883; HMM-score: 400.4)
    and 14 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 112)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase T (TIGR01882; EC 3.4.11.-; HMM-score: 85.9)
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 82.5)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 76.6)
    Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 75.6)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 64.9)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 55)
    N-acetyl-ornithine/N-acetyl-lysine deacetylase (TIGR01902; HMM-score: 38)
    putative selenium metabolism hydrolase (TIGR03526; HMM-score: 36.9)
    amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 26)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides dipeptidase PepV (TIGR01886; EC 3.4.13.-; HMM-score: 25.2)
    hydrolase, peptidase M42 family (TIGR03106; HMM-score: 21.2)
    putative dipeptidase (TIGR01887; EC 3.4.13.-; HMM-score: 20.7)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01801; EC 5.4.99.5; HMM-score: 12.6)
  • TheSEED  :
    • Peptidase T (EC 3.4.11.4)
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 84.1)
    and 3 more
    Peptidase_M42; M42 glutamyl aminopeptidase (PF05343; HMM-score: 61)
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 49.7)
    Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 41.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: a divalent metal cation
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004919
    • TAT(Tat/SPI): 0.000424
    • LIPO(Sec/SPII): 0.000471
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKEDEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSDGTTILGADDKAGLSAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDADFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKLGQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASELGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGIPSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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