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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01766
  • pan locus tag?: SAUPAN004274000
  • symbol: SAOUHSC_01766
  • pan gene symbol?: folC
  • synonym:
  • product: folylpolyglutamate synthase/dihydrofolate synthase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01766
  • symbol: SAOUHSC_01766
  • product: folylpolyglutamate synthase/dihydrofolate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1664392..1665663
  • length: 1272
  • essential: yes [1] DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATTACCTAGAGAGCTTGTATTGGATACACGAAAGAACTAAATTTGGCATCAAACCA
    GGTGTTAAACGTATGGAATGGATGCTAGCACAATTTAATAATCCTCAAAATAACATTAAG
    GGTATTCATGTAGGTGGCACAAATGGTAAAGGCTCTACAGTTGCTTACCTTAGAACAGCT
    TTAGTTGAAAATGGTTATGAAGTAGGTACATTTACGTCGCCGTTTATTGAAACATTTAAT
    GAACGAATTAGTCTAAATGGTGTGCCAATATCAAATGACGCTATTGTAGAATTAGTATCA
    CGTATTAAACCAGTAAGTGAAATGATGGAACGTGAAACAGATTTAGGTGTTGCAACTGAA
    TTCGAAATAATCACAGCGATGATGTTTTTATATTTTGGTGAAATACATCCTGTTGATTTT
    GTCATTGTTGAGGCTGGATTGGGTATAAAGAACGATTCGACAAATGTCTTTACACCGGTT
    TTATCAATCTTAACTAGTATCGGTCTAGACCATACAGATATTTTAGGTGGTACTTATCTA
    GATATTGCTAGGGATAAAGGCGCGATTATAAAGCCTAACGTTCCAGTGATATATGCTGTT
    AAAAATGAAGATGCATTAAAATATGTTCGTGAACGCGCAATTGAACAACATGCAAAGCCA
    ATTGAATTAGATAGAGAAATTGTTGTTGTATCGCAAAATGATGAATTTACTTACCGTTAT
    AAAGATTATGAATTAGAAACAATCATTTTAAGCATGTTAGGTGAACATCAGAAACAAAAT
    GCTGCATTAGCCATAACAGCTCTTATTGAATTAAATGAACAAGGATTAATTGAATTAGAT
    TTCAATAAGATGATAGACGGTATTGAATCAGTTCGTTGGACTGGACGTATTGAGCAGGTG
    CATGACAAACCTTTAATCATTTTGGATGGCGCACATAATTCAGAGAGTATAGATGCTCTA
    ATTGATACAATTAAACAGTACCATGATAAAGAAAAAGTAGATATTTTGTTCTCAGCAATA
    AACGGAAAACCGATTAACGAGATGGTCAAACATTTAAGTTTAATTGCGCATACGTTTTAT
    GCAACTGAATTTGATTTTCCGAAAGCGTTACGCAAAGAAGAAATTGTAGGTAGTATTGAA
    AATGATGAAATACAATTAGTAGATGACTACGTTGAATTTATAAAAAATTATCAAGGTGAT
    ACATTAGTAATTACCGGTAGTCTGTATTTCATAAGTGAAGTTAAATCAAAATTAGACTTT
    GAAGCATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1272

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_01766
  • symbol: SAOUHSC_01766
  • description: folylpolyglutamate synthase/dihydrofolate synthase
  • length: 423
  • theoretical pI: 4.55771
  • theoretical MW: 47856.3
  • GRAVY: -0.0959811

Function[edit | edit source]

  • reaction:
    EC 6.3.2.17?  ExPASy
    Tetrahydrofolate synthase ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 376.6)
    and 5 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 55)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 41.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 35.7)
    Cell structure Cell envelope Other poly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 19.7)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 15.1)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Dihydrofolate synthase (EC 6.3.2.12)
    and 5 more
    Folylpolyglutamate synthase (EC 6.3.2.17)
    Test - Folate  Dihydrofolate synthase (EC 6.3.2.12)
    Folylpolyglutamate synthase (EC 6.3.2.17)
    Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Colicin V and Bacteriocin Production Cluster  Dihydrofolate synthase (EC 6.3.2.12)
    Folylpolyglutamate synthase (EC 6.3.2.17)
  • PFAM:
    no clan defined Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 42.8)
    and 3 more
    Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 27.5)
    ACT (CL0070) ALS_ss_C; Small subunit of acetolactate synthase (PF10369; HMM-score: 13.8)
    P-loop_NTPase (CL0023) AAA_14; AAA domain (PF13173; HMM-score: 13.5)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.022144
    • TAT(Tat/SPI): 0.000469
    • LIPO(Sec/SPII): 0.001971
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHVGGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRIKPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPVLSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHAKPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGLIELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVEFIKNYQGDTLVITGSLYFISEVKSKLDFEAL

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]