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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01794
  • pan locus tag?: SAUPAN004309000
  • symbol: SAOUHSC_01794
  • pan gene symbol?: gapB
  • synonym:
  • product: glyceraldehyde 3-phosphate dehydrogenase 2

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01794
  • symbol: SAOUHSC_01794
  • product: glyceraldehyde 3-phosphate dehydrogenase 2
  • replicon: chromosome
  • strand: -
  • coordinates: 1691107..1692132
  • length: 1026
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCAACGAATATTGCAATTAATGGTATGGGTAGAATTGGAAGAATGGTATTACGTATT
    GCATTACAAAATAAAAATTTAAATGTAGTAGCGATAAATGCTAGTTATCCACCCGAAACA
    ATTGCACATTTAATCAATTACGATACGACACATGGAAAATATAATCTAAAAGTTGAACCG
    ATTGAAAATGGATTGCAAGTTGGAGATCATAAAATTAAATTGGTTGCTGATCGCAATCCT
    GAAAACTTGCCATGGAAAGAATTAGATATCGATATTGCTATAGATGCAACTGGTAAATTT
    AATCATGGTGATAAAGCCATCGCACATATTAAAGCAGGTGCCAAAAAAGTTTTGTTAACT
    GGTCCTTCAAAAGGTGGACATGTTCAAATGGTAGTTAAAGGCGTAAATGATAACCAATTA
    GATATAGAAGCATTTGACATTTTTAGTAATGCTTCATGTACTACTAATTGCATTGGTCCA
    GTTGCAAAAGTTTTAAATAATCAGTTTGGAATAGTTAATGGTTTAATGACTACTGTTCAC
    GCTATTACAAATGACCAAAAAAATATTGATAATCCACATAAAGATTTAAGACGTGCACGT
    TCATGTAATGAAAGCATTATTCCTACTTCTACTGGTGCGGCGAAAGCTTTAAAAGAAGTA
    TTACCAGAATTAGAAGGTAAATTACACGGCATGGCATTACGTGTACCAACAAAGAATGTA
    TCGCTCGTTGATTTAGTTGTTGATTTAGAAAAAGAAGTAACTGCAGAAGAAGTAAACCAA
    GCTTTTGAAAATGCAGGTTTAGAAGGTATCATAGAAGTCGAACATCAACCACTAGTGTCT
    GTTGATTTTAATACTAATCCAAATTCAGCTATTATTGATGCAAAATCAACAATGGTCATG
    TCAGGGAATAAAGTAAAAGTTATTGCTTGGTATGATAATGAATGGGGTTATTCAAATAGA
    GTTGTAGATGTTGCTGAGCAAATTGGAGCACTTTTGACATCAAAAGAAACTGTAAGTGCA
    AGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1026

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_01794
  • symbol: SAOUHSC_01794
  • description: glyceraldehyde 3-phosphate dehydrogenase 2
  • length: 341
  • theoretical pI: 6.35212
  • theoretical MW: 36979
  • GRAVY: -0.1739

Function[edit | edit source]

  • reaction:
    EC 1.2.1.-?  ExPASy
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis glyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 412.6)
    and 3 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine erythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 317.7)
    glyceraldehyde-3-phosphate dehydrogenase, type II (TIGR01546; EC 1.2.1.59; HMM-score: 18.2)
    Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 16.3)
  • TheSEED  :
    • Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (EC 1.2.1.13)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13)
    and 1 more
    Stress Response Oxidative stress Redox-dependent regulation of nucleus processes  NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13)
  • PFAM:
    GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 217.2)
    and 4 more
    NADP_Rossmann (CL0063) Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain (PF00044; HMM-score: 121.3)
    DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 16)
    KOW (CL0107) Ribosomal_L14e; Ribosomal protein L14 (PF01929; HMM-score: 13.4)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 12.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.67
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.056954
    • TAT(Tat/SPI): 0.000955
    • LIPO(Sec/SPII): 0.002552
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHLINYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNHGDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGPVAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALKEVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQPLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTSKETVSAS

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]