From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01797
  • pan locus tag?: SAUPAN004313000
  • symbol: SAOUHSC_01797
  • pan gene symbol?: polA
  • synonym:
  • product: DNA polymerase I

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01797
  • symbol: SAOUHSC_01797
  • product: DNA polymerase I
  • replicon: chromosome
  • strand: -
  • coordinates: 1693829..1696459
  • length: 2631
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    GTGAATAAATTAGTATTAATCGATGGTAATAGCTTAAGTTTTAGAGCTTTTTATGCATTA
    CCTTTGTTATCAAACAAAGCAGGCATTCATACCAATGCAGTATATGGTTTTGCAATGTTA
    CTAGAGAAGATTTTAAAAGAAGAAAAGCCAAATCATTTTTTAGTAGCGTTTGATGCAGGT
    AAAACGACTTTCAGACATGAAAAATATAGTGAATATAAAGGTGGACGCCAGAAAACGCCG
    CCTGAACTAAGTGAGCAATTTCCTTATATTCGCCAATTATTAGATGCTTATCATATTAAA
    CGTTATGAATTAGATAACTATGAGGCAGATGATATTATCGGAACTTTAAGTAAAGAAGCA
    GATAAAGCGGGCTTTCAGACAATTATTATTACGGGAGACCGAGATTTAACACAACTTGCA
    ACGGATAATGTAACCATTTATTACACTAAAAAAGGTGTTACTGATGTTGATCATTATACA
    CCTGACTTTATTGCGGAAAAATACAACGGGTTAACACCTAATCAAATTATTGATATGAAA
    GGATTAATGGGAGATACTTCTGATAATATACCAGGTGTTGCAGGTGTTGGAGAGAAAACA
    GCAATAAAATTGCTAAACCAATTTGACACAGTAGAAGGTGTCTATGAACATTTAGATGAA
    ATTTCAGGTAAAAAGTTAAAAGAAAAACTTCAAAATAGCAAAGAAGATGCATTAATGAGT
    AAAGAATTAGCAACGATTAATGTAGATAGTCCGATTGAAGTAAAACTTGAAGATACGTTA
    ATGACTCATCAAGATGAACAACAAGAAAAAATCGAATTGTTTAAGAAGTTAGAATTCAAA
    CAATTGTTGGCTGACATTGATCAATCAGCAAGCGTTGAAGATGCAATAGAAAAGACATTT
    GAAATTGAAACGTCTTTTGATAATATTGATTTTACTTCATTGAAAGAAGCGGCCATCCAT
    TTCGAATTAGACGGTGGTAACTATTTGCGAAATAATATTTTAAAATTCTCTTTATTTACA
    GGTGAGAAACATATTGTAATTAATGCGGATGACATAAATAATTATGTCGAACTAGTTTCA
    TGGTTAGAAAATCCGAATTCGAAAAAAGTCGTATATGATGCTAAAAAAACATATGTAGCA
    TCACATAGATTGGGGATTGATATTCAAAATATTTCTTTCGATATTATGTTGGCCAGTTAT
    ATAATTGATCCATCTCGTACGATTAGTGATGTTCAATCAGTTGTTTCATTGTACGGTCAA
    AGTTTTGTGAAAGACGATGTGAGTATATATGGAAAAGGTAAGAAATTTAAGGTACCTGAA
    GATGATGTTTTAAATCCATATGTTGCTTCTATTACTGATGCAATTTACTTTGCGAAACCA
    AATATGGATAAACAATTAGAAGAATACAATCAGGTAGAACTCTTGGCTGATTTAGAGCTA
    CCGCTAGCTAAGATTTTAAGTGAAATGGAAGAAATTGGTATATTTACAGATGTTCATGAT
    TTAGAAGAAATGGAAAAAGAAATTCAAGAAAAATTAGACGTCTTGATTCGAAATATCCAT
    GATGCAGCTGGTGAAGATTTTAATATAAATTCTCCTAAGCAATTAGGTGTTGTATTGTTT
    GAGACATTACAATTACCTGTTATTAAAAAGACGAAAACAGGATATTCTACTGCTGTAGAT
    GTCTTAGAGCAATTGCAAGGTGAACATCCTATCATTGATTATATTTTAGAATATCGTCAA
    TTATCTAAGTTACAGTCTACTTATGTTGAAGGATTGCAAAAGGTAATTAGTGATGATCAA
    CGTATCCATACACGTTTTAATCAAACTTTAGCGCAAACTGGACGTTTATCAAGTGTAGAT
    CCTAATTTACAAAATATTCCGGTTAGACTTGAAGAAGGGCGTAAAATAAGAAAAGCCTTT
    AAACCAACTTCAAAAGATAGCGTTATATTATCAGCAGATTATTCTCAAATTGAATTGCGT
    GTATTAGCACACATTACACAAGATGAGAGTATGAAAGAAGCATTTATCAACGGCGATGAT
    ATTCATACAGCAACTGCTATGAAAGTATTTGGTGTAGAAGCTGATCAAGTCGATAGTTTA
    ATGCGTCGTCAAGCAAAAGCAGTTAACTTTGGAATTGTTTATGGGATAAGTGATTATGGT
    TTAAGTCAAAGTTTAGGTATTACTCGTAAAAAAGCAAAAGCATTCATTGATGATTATTTA
    GCTAGTTTCCCAGGTGTAAAACAATATATGTCTGATATTGTAAAAGATGCCAAAGCTTTA
    GGTTACGTGGAAACATTGCTACATCGTCGACGCTATATTCCTGATATTACGAGTCGTAAC
    TTTAATTTACGCGGCTTTGCTGAACGTACTGCTATGAATACGCCAATACAGGGCAGTGCT
    GCAGATATCATTAAACTGGCAATGGTTAAATTTGCTCAAAAAATGAAAGAGACAACATAT
    CAAGCTAAACTATTATTACAAGTACACGATGAATTAATTTTTGAAGTACCTAAGTCAGAA
    GTAGATTCATTTAGTGAATTTGTAGAAGAGATAATGGAAAATGCATTGCAATTAGATGTT
    CCATTAAAAGTAGACTCAAGTTATGGTGCAACCTGGTATGATGCAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2631

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01797
  • symbol: SAOUHSC_01797
  • description: DNA polymerase I
  • length: 876
  • theoretical pI: 4.77368
  • theoretical MW: 99194
  • GRAVY: -0.32911

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase I (TIGR00593; EC 2.7.7.7; HMM-score: 1054.3)
    and 1 more
    flap structure-specific endonuclease (TIGR03674; EC 3.1.-.-; HMM-score: 19.4)
  • TheSEED  :
    • DNA polymerase I (EC 2.7.7.7)
    DNA Metabolism DNA repair DNA Repair Base Excision  DNA polymerase I (EC 2.7.7.7)
    and 1 more
    DNA Metabolism DNA replication DNA-replication  DNA polymerase I (EC 2.7.7.7)
  • PFAM:
    no clan defined DNA_pol_A; DNA polymerase family A (PF00476; HMM-score: 520.6)
    and 4 more
    PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 183.2)
    5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 122)
    RNase_H (CL0219) DNA_pol_A_exo1; 3'-5' exonuclease (PF01612; HMM-score: 23.2)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 16.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010601
    • TAT(Tat/SPI): 0.001762
    • LIPO(Sec/SPII): 0.001828
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKILKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRYELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYTPDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHLDEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKKLEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNILKFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNISFDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVASITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEIQEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQGEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQNIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIHTATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYLASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQGSAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENALQLDVPLKVDSSYGATWYDAK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_01797&oldid=101950"