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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02371
  • pan locus tag?: SAUPAN005426000
  • symbol: SAOUHSC_02371
  • pan gene symbol?: coaA
  • synonym:
  • product: pantothenate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02371
  • symbol: SAOUHSC_02371
  • product: pantothenate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 2193931..2194734
  • length: 804
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAAGTTGGCATTGACGCTGGCGGTACACTAATTAAAATCGTTCAAGAGCAAGATAAC
    CAACGTACTTTTAAAACTGAATTAACTAAAAATATTGATCAAGTTGTGGAATGGTTAAAC
    CAACAGCAAATTGAAAAATTATGCTTAACTGGAGGTAATGCAGGTGTCATTGCTGAAAAC
    ATCAACATTCCTGCACAAATTTTTGTAGAGTTTGATGCTGCATCTCAAGGACTAGGTATT
    TTGTTAAAAGAGCAAGGCCATGACTTAGCAGATTATATTTTTGCAAATGTTGGTACCGGT
    ACGTCACTACATTATTTCGACGGCCAATCACAACGTCGTGTAGGCGGTATCGGTACAGGT
    GGAGGCATGATTCAAGGACTCGGTTATTTACTAAGTCAAATCACTGATTATAAACAGTTA
    ACTGACATGGCACAACACGGTGATCGTAACACTATCGATTTAAAGGTTAGACATATTTAT
    AAAGATACCGAACCACCCATTCCCGGTGATTTAACAGCAGCTAATTTTGGTCATGTACTA
    CATCATTTAGATGCTGATTTTACACCTAGCAATAAATTAGCAGCCGTTATTGGTGTCGTT
    GGCGAAGTTGTTACGACAATGGCCATTACTGTTGCACGTGAATTTAAAACTGAAAATATC
    GTTTATATCGGTTCTTCATTTCATAATAACGCTTTATTACGCAAAGTTGTGGAAGATTAT
    ACTGTGTTACGCGGTTGCAAACCGTACTATGTTGAAAACGGCGCATTTTCAGGTGCTATT
    GGTGCATTATATTTAGAAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    804

⊟Protein[edit | edit source]

Protein Data Bank: 5ELZ
Protein Data Bank: 5JIC

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02371
  • symbol: SAOUHSC_02371
  • description: pantothenate kinase
  • length: 267
  • theoretical pI: 5.55856
  • theoretical MW: 29096.8
  • GRAVY: -0.0988764

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.33?  ExPASy
    Pantothenate kinase ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A pantothenate kinase (TIGR00555; EC 2.7.1.33; HMM-score: 314.4)
    and 2 more
    Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 14.5)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 12.5)
  • TheSEED  :
    • Pantothenate kinase type II, eukaryotic (EC 2.7.1.33)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Pantothenate kinase type II, eukaryotic (EC 2.7.1.33)
  • PFAM:
    Actin_ATPase (CL0108) Fumble; Fumble (PF03630; HMM-score: 86.6)
    and 1 more
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018811
    • TAT(Tat/SPI): 0.001751
    • LIPO(Sec/SPII): 0.000446
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQIEKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTSLHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIYKDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTENIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Anthony E Choudhry, Tracy L Mandichak, John P Broskey, Richard W Egolf, Cynthia Kinsland, Tadhg P Begley, Mark A Seefeld, Thomas W Ku, James R Brown, Magdalena Zalacain, Kapila Ratnam
Inhibitors of pantothenate kinase: novel antibiotics for staphylococcal infections.
Antimicrob Agents Chemother: 2003, 47(6);2051-5
[PubMed:12760898] [WorldCat.org] [DOI] (P p)Roberta Leonardi, Shigeru Chohnan, Yong-Mei Zhang, Kristopher G Virga, Richard E Lee, Charles O Rock, Suzanne Jackowski
A pantothenate kinase from Staphylococcus aureus refractory to feedback regulation by coenzyme A.
J Biol Chem: 2005, 280(5);3314-22
[PubMed:15548531] [WorldCat.org] [DOI] (P p)

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