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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02435
  • pan locus tag?: SAUPAN005571000
  • symbol: SAOUHSC_02435
  • pan gene symbol?: sfnaA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02435
  • symbol: SAOUHSC_02435
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2260710..2261903
  • length: 1194
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACAAAATATTTTTTTAGCAGTTCTTTTCTACTATTTCTAGGTAATTGGATTGGACAA
    ATAGGGCTAAATTGGTTTGTACTTACCACTTATCATAACGCAGTTTATCTGGGGATTGTC
    AATTTTTGCAGACTTGTACCAATATTATTACTAAGTGTGTGGGCAGGGGCAATTGCCGAT
    AAATATGATAAAGGGCGATTGCTGAGAATTACAATTTCATCATCATTTTTAGTAACTGCA
    ATTTTATGTGTGCTCACGTATAGTTTCACTGCAATTCCAATTAGCGTCATTATTATATAT
    GCGACATTAAGAGGGATTTTAAGTGCGGTTGAAACACCTTTAAGACAAGCAATCTTACCA
    GATTTATCAGATAAAATATCTACTACACAAGCTGTATCATTTCATTCATTCATCATTAAT
    ATTTGTCGTTCAATAGGGCCTGCCATTGCTGGTGTCATATTAGCAGTCTATCATGCGCCA
    ACAACATTTCTTGCACAGGCAATTTGTTATTTTATCGCAGTTTTATTATGCTTACCATTA
    CATTTTAAAGTAACTAAAATACCTGAAGATGCATCAAGATACATGCCGTTAAAAGTTATT
    ATAGATTACTTCAAATTACATATGGAAGGTCGACAAATATTTATAACATCATTATTGATT
    ATGGCGACAGGTTTTTCATATACGACACTTTTACCAGTTTTGACAAACAAAGTATTTCCG
    GGGAAATCTGAAATATTTGGTATCGCTATGACGATGTGTGCCATTGGTGGTATTATTGCA
    ACGCTAGTTTTACCTAAAGTACTTAAATATATTGGTATGGTAAATATGTATTATTTAAGT
    TCATTTTTATTTGGCATTGCTTTGTTAGGTGTGGTATTTCACAATATTGTCATCATGTTC
    ATTTGTATTACATTGATTGGGTTATTTAGTCAATGGGCACGTACGACAAATCGCGTTTAT
    TTTCAAAATAATGTTAAAGATTATGAACGTGGTAAAGTACTGAGTATTATTATGATGGAT
    AGAGGTATGATTCCATTGGGAAGTCTATTAATGAGTATATGTGCAGATGTGTTTGGCATT
    GTTAGAACTTTTTCAATAATGGGAATAAGTACTATATGCATTACAATGGTATTCTATTTT
    ATAAATAGAAAGTTGAAGTTAAAGTTGGAGGAAAGTAATCATGGTATATCTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1194

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02435
  • symbol: SAOUHSC_02435
  • description: hypothetical protein
  • length: 397
  • theoretical pI: 9.65741
  • theoretical MW: 44337.1
  • GRAVY: 0.86801

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 123.6)
    and 1 more
    multidrug resistance protein (TIGR00880; HMM-score: 31.4)
  • TheSEED  :
    • Macrolide-efflux protein
  • PFAM:
    MFS (CL0015) MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 141.6)
    and 2 more
    MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 100.5)
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009055
    • TAT(Tat/SPI): 0.00056
    • LIPO(Sec/SPII): 0.01214
  • predicted transmembrane helices (TMHMM): 11

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCRLVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYATLRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAPTTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSLLIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNMYYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVLSIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEESNHGIS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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