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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02463
  • pan locus tag?: SAUPAN005610000
  • symbol: SAOUHSC_02463
  • pan gene symbol?: hysA
  • synonym:
  • product: hyaluronate lyase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02463
  • symbol: SAOUHSC_02463
  • product: hyaluronate lyase
  • replicon: chromosome
  • strand: +
  • coordinates: 2284877..2287300
  • length: 2424
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACATATAGAATAAAGAAATGGCAAAAATTATCCACCATTACGTTATTAATGGCTGGT
    GTGATTACTTTGAATGGTGGTGAATTCAGAAGTGTTGATAAACATCAAATCGCTGTGGCT
    GATACGAATGTTCAAACGCCAGATTATGAAAAATTGAGGAACACATGGCTGGACGTTAAC
    TATGGTTATGATAAGTATGATGAGAATAATCCAGATATGAAGAAGAAGTTTGATGCTACA
    GAGAAAGAGGCGACGAATTTACTCAAGGAAATGAAAACTGAAAGTGGTAGGAAATACTTG
    TGGTCAGGAGCGGAAACCCTTGAAACTAATTCTTCTCATATGACTCGTACCTATCGTAAT
    ATTGAGAAAATCGCAGAAGCGATGAGGAATCCTAAAACCACTTTAAATACTGACGAAAAT
    AAGAAGAAAGTGAAAGATGCCCTTGAGTGGCTGCATAAAAATGCATATGGAAAAGAACCA
    GATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAAC
    TTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTG
    CTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTC
    GCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGA
    AATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGAT
    ATGATGAAAAAGTCTATAGATTCATTTAATAAAGTCTTCACTTACGTTCAAGATTCTGCC
    ACTGGTAAAGAACGTAATGGATTTTATAAAGATGGCTCTTACATTGATCATCAAGACGTT
    CCATACACTGGTGCTTATGGCGTTGTACTCTTAGAGGGGATTTCTCAAATGATGCCGATG
    ATAAAAGAAACACCTTTTAATGATAAAACCCAAAATGATACAACCTTAAAGTCATGGATT
    GACGACGGATTTATGCCACTCATTTATAAAGGTGAAATGATGGATTTATCACGAGGTAGA
    GCTATCAGTCGTGAAAATGAAACCAGTCACTCAGCATCTGCAACAGTAATGAAATCATTG
    TTGAGATTAAGTGATGCCATGGATGATTCAACAAAAGCTAAGTATAAAAAGATTGTCAAA
    TCTTCAGTAGAGTCAGATTCAAGTTATAAACAAAATGATTATTTAAATTCATATTCGGAC
    ATAGATAAAATGAAGTCTTTAATGACAGATAACAGTATTTCTAAAAACGGATTAACACAA
    CAACTTAAAATATATAATGACATGGATCGTGTCACCTATCATAACAAAGACTTAGACTTT
    GCATTTGGTTTAAGTATGACGTCGAAAAACGTAGCACGCTATGAAAGTATCAACGGAGAG
    AATTTAAAAGGTTGGCACACTGGTGCTGGAATGTCTTATTTATATAACAGCGATGTCAAA
    CACTATCATGATAACTTCTGGGTGACAGCCGATATGAAACGTTTATCAGGTACAACAACT
    TTAGACAATGAAATATTAAAAGATACGGATGATAAAAAGTCGAGTAAAACTTTTGTTGGC
    GGAACAAAAGTTGATGACCAACATGCTAGTATCGGAATGGATTTTGAAAATCAGGACAAA
    ACTTTAACTGCCAAAAAATCATATTTCATATTAAACGATAAAATTGTCTTCTTAGGAACT
    GGCATTAAAAGTACTGATTCATCAAAGAATCCAGTTACAACGATTGAAAATCGCAAAGCG
    AATGGGTATACGTTATATACAGACGATAAACAAACAACCAATTCTGATAATCAGGAAAAC
    AATTCAGTCTTTTTAGAGTCCACAGATACCAAAAAGAACATCGGTTATCATTTTTTAAAC
    AAACCGAAAATAACTGTAAAAAAAGAAAGTCATACTGGTAAGTGGAAAGAAATAAATAAA
    AGTCAAAAGGATACACAAAAAACTGATGAGTATTATGAAGTAACTCAAAAGCATTCTAAT
    TCTGACAATAAATATGGATATGTGTTGTATCCAGGCTTATCTAAAGATGTCTTTAAGACA
    AAAAAAGATGAAGTAACTGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAAT
    GAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAAC
    ACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACT
    TATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAA
    ATTTCAATGACCGGTTACTCTATTACAAACAAAAATACGTCGACTTCTAATGAATCCGGC
    GTGCACTTTGAATTAACTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2424

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02463
  • symbol: SAOUHSC_02463
  • description: hyaluronate lyase
  • length: 807
  • theoretical pI: 7.79135
  • theoretical MW: 91984.3
  • GRAVY: -0.878934

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.2.1?  ExPASy
    Hyaluronate lyase Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
  • TIGRFAM:
    Hypothetical proteins Conserved TIGR02680 family protein (TIGR02680; HMM-score: 12.9)
  • TheSEED  :
    • Hyaluronate lyase precursor (EC 4.2.2.1)
  • PFAM:
    Hy-ly_N (CL0372) Lyase_8_N; Polysaccharide lyase family 8, N terminal alpha-helical domain (PF08124; HMM-score: 300.8)
    and 2 more
    Gal_mutarotase (CL0103) Lyase_8; Polysaccharide lyase family 8, super-sandwich domain (PF02278; HMM-score: 232)
    no clan defined Lyase_8_C; Polysaccharide lyase family 8, C-terminal beta-sandwich domain (PF02884; HMM-score: 37.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 40 aa
    • SP(Sec/SPI): 0.641661
    • TAT(Tat/SPI): 0.005709
    • LIPO(Sec/SPII): 0.167847
    • Cleavage Site: CS pos: 40-41. AVA-DT. Pr: 0.3142
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNVQTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWSGAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEPDKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIKTFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYVQDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDTTLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTKAKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDRVTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWVTADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKKSYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVFLESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSDNKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINNTKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNESGVHFELTK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

A M Farrell, D Taylor, K T Holland
Cloning, nucleotide sequence determination and expression of the Staphylococcus aureus hyaluronate lyase gene.
FEMS Microbiol Lett: 1995, 130(1);81-5
[PubMed:7557301] [WorldCat.org] [DOI] (P p)

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