From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02671
  • pan locus tag?: SAUPAN005915000
  • symbol: SAOUHSC_02671
  • pan gene symbol?: narK
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02671
  • symbol: SAOUHSC_02671
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2455468..2456637
  • length: 1170
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTACAAAACAAAAGGTGGCTTTCAACTTACATTACAAACATTAAGTTTAGTGGTTGGG
    TTTATGGCTTGGAGTATAATTGCGCCATTAATGCCCTTTATTAAACAAGATGTCAATGTT
    ACTGAAGGTCAAATATCAATCATTTTAGCGATACCAGTTATTTTGGGATCGGTGCTCCGT
    GTGCCATTTGGTTATTTAACAAACATTGTTGGCGCTAAATGGGTATTCTTTACTAGTTTT
    ATCGTATTGTTATTCCCGATATTTTTCTTAAGCCAAGCACAAACACCGGGTATGTTAATG
    GCTTCAGGATTTTTCCTTGGTGTAGGTGGTGCAATTTTCTCAGTTGGTGTTACATCAGTT
    CCTAAATATTTCCCTAAAGAAAAAGTAGGTCTAGCAAATGGTATTTATGGTATGGGAAAT
    ATCGGTACAGCAGTTTCTTCATTTTTAGCACCACCGATAGCGGGTATTATTGGTTGGCAA
    ACAACAGTTAGAAGTTACTTAATTATTATCGCTTTATTTGCATTAATTATGTTCATTTTT
    GGTGACACACAAGAACGTAAAATTAAAGTACCATTAATGGCTCAAATGAAAACATTATCT
    AAAAACTACAAATTATATTACTTAAGTTATTGGTATTTTATTACTTTTGGTGCTTTTGTA
    GCATTTGGTATTTTCTTACCTAACTACTTAGTAAATCATTTTGGAATTGACAAAGTAGAT
    GCTGGTATTCGATCAGGTGTATTCATTGCGCTGGCAACATTCTTAAGACCAATAGGTGGC
    ATTTTAGGTGATAAATTTAATGCAGTTAAAGTATTGATGATTGATTTTGTTGTTATGATT
    ATCGGTGCCATTATTTTAGGTATTTCAGACCATATCGCATTATTCACTGTAGGCTGTTTA
    ACAATAAGTATTTGTGCAGGTATTGGTAACGGCTTAATCTTCAAATTAGTACCATCATAC
    TTCTTAAATGAAGCGGGATCCGCAAATGGTATCGTATCAATGATGGGTGGTTTAGGAGGA
    TTCTTCCCACCACTAGTAATCACGTACGTAGCTAATTTAACAGGATCAAGTCATTTAGCA
    TTTATTTTCTTAGCGGTATTTGGATGTATTGCATTATTTACCATGCGTCATTTATACCAA
    AAAGAATATGGCTCATTGAAAAACGGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1170

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02671
  • symbol: SAOUHSC_02671
  • description: hypothetical protein
  • length: 389
  • theoretical pI: 9.92595
  • theoretical MW: 42148.2
  • GRAVY: 0.921851

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 197.7)
    and 9 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 58.2)
    Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 58)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 50.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 44.7)
    multidrug resistance protein (TIGR00880; HMM-score: 36.3)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
    oxalate/formate antiporter (TIGR04259; HMM-score: 21.7)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 15.3)
  • TheSEED  :
    • Nitrate/nitrite transporter
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitrate and nitrite ammonification  Nitrate/nitrite transporter
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 150.3)
    and 3 more
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 19)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 11.5)
    MFS (CL0015) MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 8.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.070673
    • TAT(Tat/SPI): 0.001376
    • LIPO(Sec/SPII): 0.020064
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQTTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, NreC* (activation) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise 
    NreC*(TF)important in Nitrate and nitrite respiration; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_02671&oldid=98922"