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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02681
  • pan locus tag?: SAUPAN005928000
  • symbol: SAOUHSC_02681
  • pan gene symbol?: narG
  • synonym:
  • product: nitrate reductase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02681
  • symbol: SAOUHSC_02681
  • product: nitrate reductase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 2463891..2467580
  • length: 3690
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGAAAATTTGGATTGAATTTCTTTAAGCCAACAGAAAAATTTAATGGGAATTGGTCG
    ATCCTAGAAAGTAAAAGTAGAGAATGGGAAAAAATGTACAGAGAACGTTGGAGCCACGAT
    AAAGAAGTAAGAACAACACATGGTGTTAACTGTACAGGCTCATGTTCTTGGAAAGTATTT
    GTGAAAAATGGTGTGATTACCTGGGAAAATCAACAAACTGACTATCCAAGTTGTGGTCCG
    GATATGCCTGAATATGAACCGAGAGGATGTCCACGAGGTGCGTCATTCTCTTGGTATGAA
    TACAGTCCGCTTCGAATCAAATATCCATATATTCGTGGAAAACTCTGGGATTTATGGACT
    GAAGCATTAGAAGAAAACAATGGTAATCGCGTTGCTGCATGGGCGTCTATTGTTGAAAAT
    GAAGACAAAGCCAAACAATATAAGCAAGCCCGAGGTATGGGAGGGCACGTGCGTTCAAAT
    TGGAAAGACGTTACAGAGATAATCGCAGCACAATTACTGTATACAATAAAAAAATATGGT
    CCAGATCGAATCGCAGGATTTACACCTATTCCAGCGATGTCAATGATTAGTTATGCAGCA
    GGTGCTCGATTCATCAATTTGCTTGGTGGTGAAATGCTTAGTTTTTATGACTGGTATGCA
    GATTTACCACCTGCCTCTCCACAAATTTGGGGAGAGCAAACAGATGTGCCTGAATCAAGT
    GACTGGTATAACGCATCATACATTATTATGTGGGGCTCTAATGTACCTTTAACACGTACT
    CCGGATGCACATTTTATGACTGAAGTCCGCTATAAAGGTACAAAAGTCATTTCAGTAGCA
    CCAGATTACGCAGAAAATGTGAAATTTGCAGATAACTGGCTAGCACCGAATCCTGGTTCA
    GATGCTGCAATTGCACAAGCAATGACGCATGTTATTTTACAAGAACATTATGTTAATCAA
    CCTAATGAACGCTTTATAAATTACGCTAAACAATATACAGATATGCCGTTTCTTATCATG
    CTGGATGAAGATGAAAATGGATATAAAGCGGGTCGATTTTTAAGAGCGAGTGACTTAGGT
    CAAACAACAGAGCAAGGCGAATGGAAGCCAGTTATTCATGATGCAATCAGCGATAGTTTA
    GTAGTACCTAATGGCACAATGGGTCAACGTTGGGAAGAAGGTAAGAAGTGGAACTTAAAA
    CTAGAAACAGAAGATGGTTCTAAAATTAACCCTACATTATCAATGACAGAAGGTGGATAC
    GAATTAGAAACAATTCAATTCCCATACTTTGATAGTGATGGAGATGGGATATTCAATCGT
    CCAATTCCAACTCGACAAGTCACTTTAGCAAATGGTGACAAAGTCCGTATTGCTACAATT
    TTTGACTTAATGGCAAGTCAATATGGCGTGCGTCGTTTTGATCATAAATTAGAATCAAAA
    GGATACGACGATGCAGAATCAAAATATACACCTGCTTGGCAAGAAGCCATTTCAGGCGTA
    AAACAAAGTGTTGTCATTCAAGTAGCGAAAGAATTTGCGCAAAACGCTATCGATACTGAA
    GGGCGTTCAATGATTATCATGGGTGCGGGTATTAACCATTGGTTTAACTCAGATACGATT
    TATCGTTCAATCTTAAACTTAGTTATGTTATGTGGCTGTCAAGGTGTGAATGGTGGCGGT
    TGGGCTCACTATGTGGGACAAGAAAAATGTCGTCCGATTGAAGGATGGAGTACTGTCGCA
    TTTGCGAAAGACTGGCAAGGACCACCACGTTTGCAAAACGGAACAAGTTGGTTCTATTTT
    GCAACAGACCAATGGAAATATGAAGAGTCAAATGTAGATCGTTTAAAATCTCCATTAGCT
    AAAACAGAGGAGTTAAAGCATCAACATCCAGCTGATTATAATGTTTTAGCAGCTAGACTT
    GGTTGGTTACCATCATATCCACAATTTAATAAAAATAGTTTGTTGTTTGCAGAAGAAGCT
    AAAGATGAAGGTATAGATTCAAATGAAGCGATTTTGCAACGTGCAATTGATGAAGTGAAA
    TCAAAACAAACACAATTTGCAATAGAAGATCCTGATTTGAAAAAGAATCATCCAAAATCA
    TTATTTATATGGCGTTCAAACTTAATTTCAAGTTCTGCAAAAGGTCAAGAATACTTTATG
    AAGCATTTACTTGGCACAAAATCAGGGTTATTAGCTACACCAAATGAAGATGAAAAGCCA
    GAAGAAATTACGTGGCGTGAAGAAACAACAGGTAAGTTAGATTTAGTGGTCTCTTTAGAC
    TTCAGAATGACGGCAACACCGTTATATTCTGACATTGTTTTGCCGGCAGCGACTTGGTAT
    GAAAAACATGATTTATCATCAACGGATATGCATCCATATGTACATCCTTTCAATCCTGCC
    ATTGATCCGTTATGGGAATCGCGTTCAGACTGGGATATTTATAAAACGTTGGCAAAAGCA
    TTTTCAGAAATGGCAAAAGACTATTTACCTGGAACGTTTAAAGATGTTGTGACAACTCCA
    CTTAGTCATGATACAAAGCAAGAAATTTCAACACCATACGGCGTAGTGAAAGATTGGTCG
    AAGGGTGAAATTGAAGCGGTACCTGGACGTACAATGCCTAACTTTGCAATTGTAGAACGC
    GACTACACTAAAATTTACGACAAATATGTCACGCTTGGTCCTGTACTTGAAAAAGGGAAA
    GTTGGAGCACATGGTGTAAGTTTCGGTGTCAGTGAACAATATGAAGAATTAAAAAGTATG
    TTAGGTACGTGGAGTGATACAAATGATGATTCTGTGAGAGCGAATCGTCCACGTATTGAT
    ACAGCACGTAATGTAGCTGATGCAATACTAAGTATTTCATCTGCTACGAATGGTAAATTA
    TCACAAAAATCATATGAAGATCTCGAAGAACAAACTGGAATGCCGTTAAAAGATATTTCT
    AGCGAACGCGCTGCTGAGAAAATTTCGTTTTTAAATATAACTTCACAACCACGAGAAGTA
    ATACCGACAGCAGTATTCCCAGGTTCAAATAAACAAGGTCGACGATATTCACCATTTACA
    ACGAATATAGAACGTCTAGTACCTTTTAGAACATTAACAGGACGTCAAAGTTATTATGTG
    GATCACGAAGTTTTCCAACAATTTGGGGAGAGCTTACCAGTATATAAACCGACATTGCCG
    CCAATGGTATTTGGGAATAGAGATAAGAAAATTAAAGGTGGTACAGATGCTTTGGTACTG
    CGTTATTTAACGCCTCATGGAAAATGGAATATACACTCAATGTATCAAGATAATAAGCAT
    ATGTTGACACTATTTAGAGGTGGTCCAACGGTTTGGATATCAAATGAAGATGCTGAAAAA
    CACGATATCCAAGATAATGATTGGCTAGAAGTGTATAACCGTAATGGTGTTGTAACGGCA
    AGAGCAGTTATTTCGCATCGTATGCCTAAAGGTACAATGTTTATGTATCATGCACAAGAT
    AAACATATTCAAACGCCTGGGTCAGAAATTACAGATACACGTGGTGGTTCACACAACGCG
    CCGACTAGAATCCATTTGAAACCAACACAACTAGTCGGAGGATACGCACAAATTAGTTAT
    CACTTTAATTATTATGGACCAATTGGGAACCAAAGGGATTTATATGTAGCAGTTAGAAAG
    ATGAAGGAGGTTAATTGGCTTGAAGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
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    1260
    1320
    1380
    1440
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    1560
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    1680
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    1920
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    2040
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    2160
    2220
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    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3690

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02681
  • symbol: SAOUHSC_02681
  • description: nitrate reductase subunit alpha
  • length: 1229
  • theoretical pI: 6.19853
  • theoretical MW: 139828
  • GRAVY: -0.592596

⊟Function[edit | edit source]

  • reaction:
    EC 1.7.99.4?  ExPASy
    Nitrate reductase Nitrite + acceptor = nitrate + reduced acceptor
  • TIGRFAM:
    Metabolism Energy metabolism Anaerobic nitrate reductase, alpha subunit (TIGR01580; EC 1.7.99.4; HMM-score: 1838.2)
    and 13 more
    DMSO reductase family type II enzyme, molybdopterin subunit (TIGR03479; HMM-score: 591.5)
    anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family (TIGR02166; HMM-score: 180.3)
    formate dehydrogenase, alpha subunit (TIGR01591; EC 1.2.1.2; HMM-score: 132.8)
    molybdopterin guanine dinucleotide-containing S/N-oxide reductases (TIGR00509; HMM-score: 102.5)
    trimethylamine-N-oxide reductase TorA (TIGR02164; EC 1.7.2.3; HMM-score: 78.6)
    Metabolism Energy metabolism Anaerobic formate dehydrogenase-N alpha subunit (TIGR01553; EC 1.1.5.6; HMM-score: 46.7)
    Metabolism Energy metabolism Electron transport formate dehydrogenase-N alpha subunit (TIGR01553; EC 1.1.5.6; HMM-score: 46.7)
    Metabolism Energy metabolism Electron transport arsenite oxidase, large subunit (TIGR02693; EC 1.20.9.1; HMM-score: 28.3)
    Metabolism Central intermediary metabolism Nitrogen metabolism periplasmic nitrate reductase, large subunit (TIGR01706; EC 1.7.99.4; HMM-score: 24.6)
    Metabolism Energy metabolism Electron transport periplasmic nitrate reductase, large subunit (TIGR01706; EC 1.7.99.4; HMM-score: 24.6)
    Metabolism Energy metabolism Aerobic periplasmic nitrate reductase, large subunit (TIGR01706; EC 1.7.99.4; HMM-score: 24.6)
    oxidoreductase alpha (molybdopterin) subunit (TIGR01701; HMM-score: 19.7)
    Metabolism Energy metabolism Electron transport NADH dehydrogenase (quinone), G subunit (TIGR01973; EC 1.6.99.5; HMM-score: 13.9)
  • TheSEED  :
    • Respiratory nitrate reductase (quinol), alpha subunit (EC 1.7.5.1)
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitrate and nitrite ammonification  Respiratory nitrate reductase alpha chain (EC 1.7.99.4)
  • PFAM:
    no clan defined Molybdopterin; Molybdopterin oxidoreductase (PF00384; HMM-score: 539.6)
    and 3 more
    AcetylDC-like (CL0332) Molydop_binding; Molydopterin dinucleotide binding domain (PF01568; HMM-score: 75.9)
    4Fe-4S (CL0344) Nitr_red_alph_N; Respiratory nitrate reductase alpha N-terminal (PF14710; HMM-score: 38.9)
    Molybdop_Fe4S4; Molybdopterin oxidoreductase Fe4S4 domain (PF04879; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mo-bis(molybdopterin guanine dinucleotide), [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.069378
    • TAT(Tat/SPI): 0.00154
    • LIPO(Sec/SPII): 0.003017
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVRTTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYSPLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSNWKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDWYADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKVISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTDMPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWEEGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLANGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQVAKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVGQEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEELKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSKQTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKPEEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFNPAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVVKDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQYEELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQTGMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFRTLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGKWNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISHRMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNYYGPIGNQRDLYVAVRKMKEVNWLED

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, NreC* (activation) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise 
    NreC*(TF)important in Nitrate and nitrite respiration; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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