From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02947
  • pan locus tag?: SAUPAN006326000
  • symbol: SAOUHSC_02947
  • pan gene symbol?: cysJ
  • synonym:
  • product: sulfite reductase (NADPH) flavoprotein subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02947
  • symbol: SAOUHSC_02947
  • product: sulfite reductase (NADPH) flavoprotein subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 2710641..2712521
  • length: 1881
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    TTGAAATTAAATACATCTAATAGTCCATTTACAGAAAAACAAGTCACAGAGATTAACAAT
    CTGCTGCAAACATTAACAGAGAGCCAACAACAGTGGCTTAGTGGTTTTTTACTAGCTAAT
    AGTAACGATACGACAAGTGATAGTAATCAACAACAATTAGAGACAGAAGTGTGGCAACAA
    TCACAAATATCAGAAGAACAAGCAACTTCAACAACGTATATGTTACAAAATAAAGAGCCA
    CATATCGAAGCTAATCAGCGGCATGTTACAGTGCTATATGGTTCTGAATCAGGTAATGCC
    ATGCGTTTAGCTGAAATTTTTTCAGAACGTTTAAGTGATATCGGACATCAAGTTGTTTTG
    ATGTCAATGGATGAATATGATACGACAAACATCGCGCAGTTAGAAGATTTATTTATTATT
    ACGTCTACTCATGGTGAAGGAGAACCGCCTGATAATGCATGGGATTTCTTTGAATTTTTA
    GAAGACGATAACGCACCTAATTTAAATCATGTGAGATATTCAGTACTAGCTTTAGGTGAT
    CAAACATATGAATTTTTCTGTCAAGCCGGTAAAGATGTAGATGTTTTACTAGAAAATCTA
    GGCGCTGAGCGTATATGTAAGCGTGTAGATTGTGATATTGATTATGAAGAAGACGCAGAA
    AAGTGGATGGCAGACATCATTAATATTATTGATACCACATCAGAAGGTATTCAAAGTGAA
    TCGGTGATAAGTGAATCAATTAAGTCTGCCAAAGAAAAGAAATATTCTAAATCAAATCCA
    TACCAAGCAGAAGTATTAGCGAATATCAATTTAAATGGTACCGATTCAAATAAAGAAACA
    CGACATATAGAATTTTTACTTGATGATTTTAGTGAATCATATGAACCAGGAGATTGTATA
    GTAGCATTACCGCAAAACGACCCTGAATTGGTTGAAAAACTAATATCCATGTTAGGTTGG
    GATCCGCAATCTCCGGTGCCAATTAATGATCATGGTGATACAGTTCCTATTGTTGAAGCA
    CTAACATCACATTTTGAATTTACTAAATTAACATTGCCATTATTGAAAAATGCAGATATC
    TATTTTGACAATGAAGAATTATCTGAACGTATTCAAGATGAGTCATGGGCGCGTGAATAT
    GTTATAAATCGGGACTTTATAGATTTAATAACAGATTTTCCAACTATAGAATTACAACCT
    GAGAATATGTATCAAATCCTTAGAAAATTACCACCAAGAGAGTATTCGATTTCTAGTAGT
    TTTATGGCAACGCCAGATGAAGTGCATATTACCGTTGGTACGGTTCGTTATCAAGCACAT
    GGACGTGAGAGAAAAGGTGTATGCTCGGTTCATTTTGCTGAGCGAATTAAACCAGGCGAT
    ATAGTACCAATTTATTTGAAGAAAAATCCGAACTTCAAATTTCCGATGAAGCAAGATATA
    CCGGTTATTATGATTGGACCAGGTACTGGAATTGCTCCTTTTAGAGCATATTTACAAGAA
    CGTGAAGAACTTGGTATGACTGGAAAAACATGGTTGTTCTTTGGTGATCAACACCGTAGT
    TCTGACTTTTTATATGAAGAAGAAATAGAAGAATGGCTTGAAAATGGAAACTTAACACGC
    GTAGATTTAGCATTTTCAAGAGACCAAGAACACAAAGAATATGTACAGCATCGTATAATG
    GAAGAAAGTAAACGTTTCAATGAATGGATTGAGCAAGGCGCAGCAATCTATATTTGTGGC
    GATGAAAAATGTATGGCGAAAGATGTCCATCAAGCCATTAAAGATGTATTGGTAAAAGAA
    CGTCATATTTCTCAAGAAGAAGCAGAGTTATTATTGCGACAAATGAAACAACAACAACGC
    TATCAACGTGATGTTTATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1881

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02947
  • symbol: SAOUHSC_02947
  • description: sulfite reductase (NADPH) flavoprotein subunit alpha
  • length: 626
  • theoretical pI: 4.2983
  • theoretical MW: 72212.1
  • GRAVY: -0.584824

⊟Function[edit | edit source]

  • reaction:
    EC 1.8.1.2?  ExPASy
    Assimilatory sulfite reductase (NADPH) H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH
  • TIGRFAM:
    sulfite reductase [NADPH] flavoprotein, alpha-component (TIGR01931; EC 1.8.1.2; HMM-score: 714.4)
    and 6 more
    Metabolism Energy metabolism Other benzoyl-CoA oxygenase/reductase, BoxA protein (TIGR03224; EC 1.14.12.21; HMM-score: 63.9)
    Metabolism Energy metabolism Electron transport flavodoxin (TIGR01753; HMM-score: 57.6)
    Metabolism Energy metabolism Other phenylacetate-CoA oxygenase/reductase, PaaK subunit (TIGR02160; HMM-score: 33.1)
    Metabolism Energy metabolism Electron transport flavodoxin (TIGR01752; HMM-score: 26.5)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfite reductase, subunit B (TIGR02911; EC 1.8.-.-; HMM-score: 17.3)
    putative ribonucleotide reductase-associated flavodoxin (TIGR01754; HMM-score: 16.2)
  • TheSEED  :
    • Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Cysteine Biosynthesis  Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2)
  • PFAM:
    Flavoprotein (CL0042) Flavodoxin_1; Flavodoxin (PF00258; HMM-score: 114.7)
    FAD_Lum_binding (CL0076) FAD_binding_1; FAD binding domain (PF00667; HMM-score: 96.4)
    and 4 more
    NAD_Ferredoxin (CL0091) NAD_binding_1; Oxidoreductase NAD-binding domain (PF00175; HMM-score: 69)
    Flavoprotein (CL0042) Flavodoxin_5; Flavodoxin domain (PF12724; HMM-score: 13)
    no clan defined ANTAR; ANTAR domain (PF03861; HMM-score: 12.1)
    Anti-adapt_IraP; Sigma-S stabilisation anti-adaptor protein (PF10796; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027189
    • TAT(Tat/SPI): 0.010256
    • LIPO(Sec/SPII): 0.001039
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFLEDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_02947&oldid=102213"