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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_1342a
  • pan locus tag?:
  • symbol: SAOUHSC_1342a
  • pan gene symbol?:
  • synonym:
  • product: large-conductance mechanosensitive channel

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_1342a
  • symbol: SAOUHSC_1342a
  • product: large-conductance mechanosensitive channel
  • replicon: chromosome
  • strand: -
  • coordinates: 1284057..1284419
  • length: 363
  • essential: unknown

Accession numbers[edit | edit source]

  • Gene ID: 16830701 NCBI
  • RefSeq: YP_008530241 NCBI
  • BioCyc: G1I0R-1256 BioCyc
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGTTAAAAGAATTCAAAGAGTTCGCCTTAAAAGGTAACGTCTTAGATTTAGCAATTGCT
    GTTGTGATGGGTGCAGCTTTCAACAAGATTATATCTTCATTAGTAGAAAATATCATTATG
    CCATTAATTGGTAAAATTTTCGGATCAGTTGATTTTGCTAAAGAATGGTCATTCTGGGGT
    ATTAAATACGGTTTATTTATCCAATCTGTTATCGACTTTATTATCATCGCGTTTGCTTTA
    TTCATCTTTGTTAAGATTGCAAATACATTAATGAAGAAAGAAGAAGCCGAAGAAGAAGCA
    GTTGTGGAAGAAAATGTTGTGTTATTAACTGAAATCAGAGATTTATTACGTGAGAAAAAA
    TAA
    60
    120
    180
    240
    300
    360
    363

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_1342a
  • symbol: SAOUHSC_1342a
  • description: large-conductance mechanosensitive channel
  • length: 120
  • theoretical pI: 4.73922
  • theoretical MW: 13616.2
  • GRAVY: 0.641667

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions large conductance mechanosensitive channel protein (TIGR00220; HMM-score: 126.6)
  • TheSEED  :
    • Large-conductance mechanosensitive channel
    Potassium metabolism Potassium metabolism - no subcategory Glutathione-regulated potassium-efflux system and associated functions  Large-conductance mechanosensitive channel
    and 1 more
    Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Large-conductance mechanosensitive channel
  • PFAM:
    no clan defined MscL; Large-conductance mechanosensitive channel, MscL (PF01741; HMM-score: 136.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019989
    • TAT(Tat/SPI): 0.002238
    • LIPO(Sec/SPII): 0.012643
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

  • GI: 542123274 NCBI
  • RefSeq: YP_008530241 NCBI
  • UniProt: P68805 UniProt
  • STRING:

Protein sequence[edit | edit source]

  • MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIGKIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVVEENVVLLTEIRDLLREKK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]