COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 27-JUN-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SAP003 [new locus tag: SA_RS00010 ]
• pan locus tag^?:
• symbol: SAP003
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAP003 [new locus tag: SA_RS00010 ]
• symbol: SAP003
• product: hypothetical protein
• replicon: pN315
• strand: -
• coordinates: 2388..3080
• length: 693
• essential: unknown

⊟Accession numbers

• Gene ID: 1122742 NCBI
• RefSeq: NP_395539 NCBI
• BioCyc: see SA_RS00010
• MicrobesOnline: 143473 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  661
  
  ATGAAAATTATAAAATTATTTTCAATAATTTCCTTGTTCCTACTGGTATTTAGCGCGAAT
  TTTTCTAATGCTTACGCAAATGAAGAACAAAAAAGTTCTTTACTAGAAAATCAAAAAGAA
  AAATTGAAAAGCAAAAACATCGAAGTATCACAAATGACCAAGAAAGAAATAAGTTTTCTA
  GAACAATTGCAAAATAAAAATGAAGATATGCAAAAATTCGAAAAGCAAATGAAAAAAGAA
  GGATTTAAAAAAATAAAGCTAGAAGACAAAAATTCTAAATTAAAATATCTAGCTACAGAA
  GCAAATGATAAAAATGCATATGGCTACATAGCTAATAACGCTTATAAAAATGAAAAAACA
  AAAGAAATTGTAGTTTCTGAAACAATATATGATAACTACAACAAAAAAATCACAAAATTC
  ATTGCCGAAAAAAGAAGTATGACTGAAAACAAAGAGGGAAAAATACTTATTAACAAAAAT
  TACGTTGAATATAAGGCTGAAAAAGTTGATAAAAACAGACCGGTAACATATGCAGGATTC
  AAATGGAACGGCAAAGCATTCGCTTGTAGTATGGCTGGACTTTATGCATGTGCTCACTAC
  TGTGGAGTATGGGCAATCGTAAATCCAATAGCCGGCGGTGTATGTGAAGCAGTTTGCGGA
  ACTGCATTTGCAGCTGGATGCTCTGTAGGTTGA
  60
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  693
  

⊟Protein

⊟General

• locus tag: SAP003 [new locus tag: SA_RS00010 ]
• symbol: SAP003
• description: hypothetical protein
• length: 230
• theoretical pI: 9.64595
• theoretical MW: 26072
• GRAVY: -0.484348

⊟Function

• TIGRFAM:
  putative immunity protein/bacteriocin (TIGR04450; HMM-score: 270.5)
  and 3 more
  Cellular processes Cellular processes Toxin production and resistance halocin C8-like bacteriocin domain (TIGR04449; HMM-score: 8.8)
  SH3 domain protein (TIGR04211; HMM-score: 4.4)
  integral membrane protein TIGR01906 (TIGR01906; HMM-score: 4.1)
• TheSEED  :
  • hypothetical protein
• PFAM:
  no clan defined DUF2730; Protein of unknown function (DUF2730) (PF10805; HMM-score: 15.6)
  and 5 more
  Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 8)
  ATP_synthase (CL0255) ATP-synt_8; ATP synthase protein 8 (PF00895; HMM-score: 7.3)
  no clan defined IncA; IncA protein (PF04156; HMM-score: 6.7)
  DUF2206; Predicted membrane protein (DUF2206) (PF09971; HMM-score: 6.7)
  LMBR1; LMBR1-like membrane protein (PF04791; HMM-score: 5.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.87
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helix: 1
• LocateP: N-terminally anchored (with CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: FSNAYANE
• SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.989144
  • TAT(Tat/SPI): 0.000746
  • LIPO(Sec/SPII): 0.008779
  • Cleavage Site: CS pos: 26-27. AYA-NE. Pr: 0.9556
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 16119203 NCBI
• RefSeq: NP_395539 NCBI
• UniProt: A0A0H3JPG2 UniProt

⊟Protein sequence

• MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSKNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTAFAAGCSVG

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications