COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 27-JUN-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SAP013 [new locus tag: SA_RS00055 ]
• pan locus tag^?:
• symbol: binL
• pan gene symbol^?: —
• synonym:
• product: DNA-invertase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAP013 [new locus tag: SA_RS00055 ]
• symbol: binL
• product: DNA-invertase
• replicon: pN315
• strand: +
• coordinates: 11288..11881
• length: 594
• essential: unknown

⊟Accession numbers

• Gene ID: 1122762 NCBI
• RefSeq: NP_395549 NCBI
• BioCyc: see SA_RS00055
• MicrobesOnline: 143483 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  
  TTGAAAATAGGTTATGCAAGAGTTTCAACTGGATTACAAAATTTAAATTTACAGGAGGAT
  CGATTAAATACATATGGTTGTGAAAAGATATTTAATGACCATATGAGTGGTTCAAAAAGT
  AAAAGACCTGGTTTAGATAAAGCAATTGAATTTGCGAGGTCAGGAGATACGATTGTTGTT
  TGGAGATTAGATAGATTGGGACGTAATATGGAGGATTTAATCACATTAGTTAATGAGCTT
  AACGAACGAGGTGTAAGTTTCCATAGTTTAGAAGAAAATATAACAATGGATAAATCAAGT
  TCAACTGGACAATTATTATTTCATTTATTCGCAGCATTTGCAGAATTTGAACGTAATTTA
  ATTTTAGAGCGTTCTTCAGCTGGCCGAATTGCAGCACGTGCTAGAGGCCGATATGGAGGC
  AGACCAGAAAAATTAAATCAAAAAGATTTAAACTTACTTAAGACACTTTATGATAATGGC
  ACGCCAATTAAGACAATTGCAGAACAGTGGCATGTTTCACGTACAACAATTTATCGTTAC
  CTCAATAAATTGGAGGACAAGGAAGATGAAAAACAAGGAGAAGTATCTAACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  594
  

⊟Protein

⊟General

• locus tag: SAP013 [new locus tag: SA_RS00055 ]
• symbol: BinL
• description: DNA-invertase
• length: 197
• theoretical pI: 8.95113
• theoretical MW: 22526.3
• GRAVY: -0.667513

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 33.3)
  and 3 more
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 19.4)
  RNA polymerase sigma-70 factor, Rhodopirellula/Verrucomicrobium family (TIGR02989; HMM-score: 16.4)
  Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 14.5)
• TheSEED  :
  • Phage DNA invertase
  • Resolvase/integrase Bin
  Phages, Prophages, Transposable elements, Plasmids Transposable elements Tn552  Resolvase/integrase Bin
• PFAM:
  no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 152.8)
  and 28 more
  HTH (CL0123) HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 64)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 27.5)
  HTH_11; HTH domain (PF08279; HMM-score: 27)
  HTH_22; HTH domain (PF13309; HMM-score: 23.5)
  Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 22.8)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 22.4)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 20.5)
  MarR_2; MarR family (PF12802; HMM-score: 20.5)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 20.1)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 19.8)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 19.7)
  MarR; MarR family (PF01047; HMM-score: 18.5)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 16.8)
  HTH_40; Helix-turn-helix domain (PF14493; HMM-score: 16.1)
  HTH_30; PucR C-terminal helix-turn-helix domain (PF13556; HMM-score: 15.9)
  HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 15.7)
  Trp_repressor; Trp repressor protein (PF01371; HMM-score: 15.6)
  UPF0122; Putative helix-turn-helix protein, YlxM / p13 like (PF04297; HMM-score: 15.3)
  no clan defined TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 14.6)
  HTH (CL0123) Terminase_5; Putative ATPase subunit of terminase (gpP-like) (PF06056; HMM-score: 13.7)
  HTH_32; Homeodomain-like domain (PF13565; HMM-score: 13.7)
  HTH_AsnC-type; AsnC-type helix-turn-helix domain (PF13404; HMM-score: 13.6)
  HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 13.1)
  TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 12.9)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 12.5)
  HTH_Tnp_4; Helix-turn-helix of DDE superfamily endonuclease (PF13613; HMM-score: 12.5)
  HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 12.1)
  GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013885
  • TAT(Tat/SPI): 0.000743
  • LIPO(Sec/SPII): 0.012243
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 16119213 NCBI
• RefSeq: NP_395549 NCBI
• UniProt: A0A0H3JPG9 UniProt

⊟Protein sequence

• MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGLDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: binL > tnp > sin

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications