From AureoWiki
Jump to navigation Jump to search

Summary[edit | edit source]

  • pan ID?: SAUPAN002518000
  • symbol?: tagD
  • synonym:
  • description?: glycerol-3-phosphate cytidylyltransferase

      descriptions from strain specific annotations:

    • glycerol-3-phosphate cytidylyltransferase
    • glycerol-3-phosphate cytidylyltransferase, putative
  • strand?: +
  • coordinates?: 3030164..3030562
  • synteny block?: BlockID0017540
  • occurrence?: in 100% of 34 strains

tarD (tagD) : glycerol-3-phosphate cytidylyltransferase [1]

• A crystal structure is available : 2B7L

TarD provides a pool of CDP-activated glycerol-3-phosphate for TarB to use for UDP-disaccharide glycerophosphate priming. Wall teichoic acids in S. aureus use ribitol exclusively as their polyol extension monomer, therefore WTA enzymes in S. aureus are assigned the "tar" nomenclature. In contrast, S. epidermidis uses glycerol as its polyol extension monomer so orthologous enzymes in S. epidermidis are assigned the "tag" nomenclature. The utilization of glycerol phosphate by TarB and TarD for polyol priming further blurs the line between the "Tar" and the "Tag" nomenclature but because the polymerizing polyol will be ribitol, the enzymes retain the "Tar" nomenclature.

Orthologs[edit | edit source]

    COL:
    SACOL0698 (tagD)
    N315:
    SA0597 (tagD)
    NCTC8325:
    Newman:
    NWMN_0611 (tagD)
    USA300_FPR3757:
    04-02981:
    SA2981_0617
    08BA02176:
    C248_0723 (tagD)
    11819-97:
    MS7_0692 (tagD)
    6850:
    RSAU_000597 (tagD)
    71193:
    ST398NM01_0720
    ECT-R 2:
    ECTR2_593 (tagD)
    ED133:
    SAOV_0676 (tagD)
    ED98:
    SAAV_0604 (tagD)
    HO 5096 0412:
    SAEMRSA15_05670 (tagD)
    JH1:
    SaurJH1_0679
    JH9:
    SaurJH9_0664
    JKD6008:
    SAA6008_00658 (tagD)
    JKD6159:
    SAA6159_00596 (tagD)
    JSNZ:
    JSNZ_000608 (tagD)
    LGA251:
    SARLGA251_05750 (tagD)
    M013:
    M013TW_0629
    MRSA252:
    SAR0651 (tagD)
    MSHR1132:
    SAMSHR1132_05870
    MSSA476:
    SAS0607
    Mu3:
    SAHV_0638 (tagD)
    Mu50:
    SAV0641 (tagD)
    MW2:
    MW0603 (tagD)
    RF122:
    SAB0591 (tagD)
    ST398:
    SAPIG0720 (tagD)
    T0131:
    SAT0131_00710 (pcrA)
    TCH60:
    HMPREF0772_12546 (tagD)
    TW20:
    SATW20_07160 (tagD)
    USA300_TCH1516:
    USA300HOU_0661 (tagD)
    VC40:
    SAVC_02840

Genome Viewer[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757

Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.307)


    COL             MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIKHKKSYYDYEQRKMMLE
    N315            MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIKHKKSYYDYEQRKMMLE
    NCTC8325        MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIKHKKSYYDYEQRKMMLE
    Newman          MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIKHKKSYYDYEQRKMMLE
    USA300_FPR3757  MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIKHKKSYYDYEQRKMMLE
                    ************************************************************

    COL             SIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFLKDKCEVIYLKRTEGISTT
    N315            SIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFLKDKCEVIYLKRTEGISTT
    NCTC8325        SIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFLKDKCEVIYLKRTEGISTT
    Newman          SIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFLKDKCEVIYLKRTEGISTT
    USA300_FPR3757  SIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFLKDKCEVIYLKRTEGISTT
                    ************************************************************

    COL             KIKQELYGKDAK
    N315            KIKQELYGKDAK
    NCTC8325        KIKQELYGKDAK
    Newman          KIKQELYGKDAK
    USA300_FPR3757  KIKQELYGKDAK
                    ************

  1. David S Badurina, Michela Zolli-Juran, Eric D Brown
    CTP:glycerol 3-phosphate cytidylyltransferase (TarD) from Staphylococcus aureus catalyzes the cytidylyl transfer via an ordered Bi-Bi reaction mechanism with micromolar K(m) values.
    Biochim Biophys Acta: 2003, 1646(1-2);196-206
    [PubMed:12637027] [WorldCat.org] [DOI] (P p)
    Desiree H Fong, Veronica C-N Yim, Michael A D'Elia, Eric D Brown, Albert M Berghuis
    Crystal structure of CTP:glycerol-3-phosphate cytidylyltransferase from Staphylococcus aureus: examination of structural basis for kinetic mechanism.
    Biochim Biophys Acta: 2006, 1764(1);63-9
    [PubMed:16344011] [WorldCat.org] [DOI] (P p)
    Ziliang Qian, Yanbin Yin, Yong Zhang, Lingyi Lu, Yixue Li, Ying Jiang
    Genomic characterization of ribitol teichoic acid synthesis in Staphylococcus aureus: genes, genomic organization and gene duplication.
    BMC Genomics: 2006, 7;74
    [PubMed:16595020] [WorldCat.org] [DOI] (I e)