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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003535000
- symbol?: rbgA
- synonym:
- description?: ribosomal biogenesis GTPase
- ribosomal biogenesis GTPase
- ribosome biogenesis GTPase YlqF
- GTPase YlqF, putative
- 50S ribosomal subunit maturation GTPase RbgA
- GTPase family
- GTPase protein
- GTPase YlqF
- GTP-binding protein
- putative GTPase protein
- putative GTP-binding protein
- ribosome biogenesis GTP-binding protein YlqF
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3874639..3875523
- synteny block?: BlockID0026120
- occurrence?: in 100% of 33 strains
rbgA (ylqF): 45S ribosome unlocking protein and ribosome-associated GTPase RbgA [1]
A crystal structure is available: 6G0Z
Ribosome-associated GTPases including Era, RsgA, RbgA, HflX, ObgE, EngA and EngB are widely-conserved, stringent response alarmone-responsive ribosomal assembly cofactors
• RbgA acts to "unlock" the 45S subunit, a stable intermediate to the 50S large ribosome subunit, exposing the EngA binding site. Alarmone binding prevents display of EngA binding site resulting in immature ribosomes.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1201 (rbgA)
08BA02176:
C248_1274
11819-97:
MS7_1201 (ylqF)
6850:
RSAU_001125
71193:
ST398NM01_1243
ECT-R 2:
ECTR2_1099
ED133:
SAOV_1246
ED98:
SAAV_1217 (rbgA)
HO 5096 0412:
SAEMRSA15_10760
JH1:
SaurJH1_1327 (rbgA)
JH9:
SaurJH9_1302 (rbgA)
JKD6008:
SAA6008_01198 (rbgA)
JKD6159:
SAA6159_01098 (rbgA)
LGA251:
SARLGA251_11540
M013:
M013TW_1181
MRSA252:
SAR1219 (rbgA)
MSHR1132:
SAMSHR1132_10860
MSSA476:
SAS1177 (rbgA)
Mu3:
SAHV_1233 (rbgA)
Mu50:
SAV1243 (rbgA)
MW2:
MW1126 (rbgA)
RF122:
SAB1107 (rbgA)
ST398:
SAPIG1243
T0131:
SAT0131_01293
TCH60:
HMPREF0772_11988 (ylqF)
TW20:
SATW20_12370
USA300_TCH1516:
USA300HOU_1174 (rbgA)
VC40:
SAVC_05380 (rbgA)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMIDEVINQKPRVVILNKK
N315 MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMIDEVINQKPRVVILNKK
NCTC8325 MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMIDEVINQKPRVVILNKK
Newman MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMIDEVINQKPRVVILNKK
USA300_FPR3757 MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMIDEVINQKPRVVILNKK
************************************************************
COL DMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIKATAEKFEREKAKGLKPRA
N315 DMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIKATAEKFEREKAKGLKPRA
NCTC8325 DMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIKATAEKFEREKAKGLKPRA
Newman DMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIKATAEKFEREKAKGLKPRA
USA300_FPR3757 DMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIKATAEKFEREKAKGLKPRA
************************************************************
COL IRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQWIKVGNALQLLDTPGILWPKF
N315 IRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQWIKVSNALQLLDTPGILWPKF
NCTC8325 IRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQWIKVGNALQLLDTPGILWPKF
Newman IRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQWIKVGNALQLLDTPGILWPKF
USA300_FPR3757 IRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQWIKVGNALQLLDTPGILWPKF
*******************************************.****************
COL EDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDLARLKSHYNIEVPEDAEIIAWFD
N315 EDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDLARLKSHYNIEVPEDAEIIAWFD
NCTC8325 EDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDLARLKSHYNIEVPEDAEIIAWFD
Newman EDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDLARLKSHYNIEVPEDAEIIAWFD
USA300_FPR3757 EDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDLARLKSHYNIEVPEDAEIIAWFD
************************************************************
COL AIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNYCFDIFKDMTEELANDANN
N315 AIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNYCFDIFKDMTEELANDANN
NCTC8325 AIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNYCFDIFKDMTEELANDANN
Newman AIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNYCFDIFKDMTEELANDANN
USA300_FPR3757 AIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNYCFDIFKDMTEELANDANN
******************************************************
- ↑ Patrick Pausch, Wieland Steinchen, Maximiliane Wieland, Thomas Klaus, Sven-Andreas Freibert, Florian Altegoer, Daniel N Wilson, Gert Bange
Structural basis for (p)ppGpp-mediated inhibition of the GTPase RbgA.
J Biol Chem: 2018, 293(51);19699-19709
[PubMed:30366986] [WorldCat.org] [DOI] (I p)Amal Seffouh, Nikhil Jain, Dushyant Jahagirdar, Kaustuv Basu, Aida Razi, Xiaodan Ni, Alba Guarné, Robert A Britton, Joaquin Ortega
Structural consequences of the interaction of RbgA with a 50S ribosomal subunit assembly intermediate.
Nucleic Acids Res: 2019, 47(19);10414-10425
[PubMed:31665744] [WorldCat.org] [DOI] (I p)Daniel J Bennison, Jose A Nakamoto, Timothy D Craggs, Pohl Milón, John B Rafferty, Rebecca M Corrigan
The Stringent Response Inhibits 70S Ribosome Formation in Staphylococcus aureus by Impeding GTPase-Ribosome Interactions.
mBio: 2021, 12(6);e0267921
[PubMed:34749534] [WorldCat.org] [DOI] (I p)N Upendra, S Krishnaveni
Conformational exploration of RbgA using molecular dynamics: Possible implications in ribosome maturation and GTPase activity in different nucleotide bound states.
J Mol Graph Model: 2022, 111;108087
[PubMed:34864321] [WorldCat.org] [DOI] (I p)Amal Seffouh, Chirstian Trahan, Tanzila Wasi, Nikhil Jain, Kaustuv Basu, Robert A Britton, Marlene Oeffinger, Joaquin Ortega
RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.
Nucleic Acids Res: 2022, 50(19);10801-10816
[PubMed:35141754] [WorldCat.org] [DOI] (I p)Dominic Arpin, Armando Palacios, Kaustuv Basu, Joaquin Ortega
The binding of RbgA to a critical 50S assembly intermediate facilitates YphC function in bacterial ribosomal assembly.
Nucleic Acids Res: 2024;
[PubMed:39658043] [WorldCat.org] [DOI] (I a)