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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003936000
- symbol?: engA
- synonym:
- description?: GTP-binding protein EngA
- GTP-binding protein EngA
- ribosome biogenesis GTPase Der
- GTP-binding protein Der
- pseudogene
- putative GTPase
- ribosome-associated GTPase EngA
- small GTP-binding domain protein
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4253923..4255235
- synteny block?: BlockID0029820
- occurrence?: in 100% of 33 strains
engA (yphC, der): 45S ribosome maturation protein and ribosome-associated GTPase EngA [1]
Ribosome-associated GTPases including Era, RsgA, RbgA, HflX, ObgE, EngA and EngB are widely-conserved, stringent response alarmone-responsive ribosomal assembly cofactors
• EngA contains two GTPase binding sites and catalyzes major conformational changes and cofactor binding events during 50S subunit maturation. Alarmone binding inhibits these conformational changes.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1431 (engA)
08BA02176:
C248_1514
11819-97:
MS7_1429 (engA)
6850:
RSAU_001345 (engA)
71193:
ST398NM01_1473
ECT-R 2:
ECTR2_1327
ED133:
SAOV_1481
ED98:
SAAV_1462 (engA)
HO 5096 0412:
SAEMRSA15_13350
JH1:
SaurJH1_1562 (engA)
JH9:
SaurJH9_1532 (engA)
JKD6008:
SAA6008_01443 (engA)
JKD6159:
SAA6159_01339 (engA)
LGA251:
SARLGA251_13870
M013:
M013TW_1422
MRSA252:
SAR1484 (engA)
MSHR1132:
SAMSHR1132_13140
MSSA476:
SAS1416 (engA)
Mu3:
SAHV_1463 (engA)
Mu50:
SAV1475 (engA)
MW2:
MW1364 (engA)
RF122:
SAB1337c
ST398:
SAPIG1473 (engA)
T0131:
SAT0131_01563
TCH60:
HMPREF0772_11731 (engA)
TW20:
SATW20_14720
USA300_TCH1516:
USA300HOU_1412 (engA)
VC40:
SAVC_06625
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYSSGEWLTHDFNIIDTGG
N315 MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYSSGEWLTHDFNIIDTGG
NCTC8325 MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYSSGEWLTHDFNIIDTGG
Newman MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYSSGEWLTHDFNIIDTGG
USA300_FPR3757 MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYSSGEWLTHDFNIIDTGG
************************************************************
COL IEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEMVAQILYKSKKPVVLAVNK
N315 IEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEMVAQILYKSKKPVVLAVNK
NCTC8325 IEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEMVAQILYKSKKPVVLAVNK
Newman IEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEMVAQILYKSKKPVVLAVNK
USA300_FPR3757 IEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEMVAQILYKSKKPVVLAVNK
*********************** ************************************
COL VDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVVSHFGEEEEDPYDEDTIRLSI
N315 VDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVVSHFGEEEEDPYDEDTIRLSI
NCTC8325 VDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVVSHFGEEEEDPYDEDTIRLSI
Newman VDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVVSHFGEEEEDPYDEDTIRLSI
USA300_FPR3757 VDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVVSHFGEEEEDPYDEDTIRLSI
************************************************************
COL IGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEYSYDGQDYVLIDTAGMRKKGKVY
N315 IGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEYSYDGQDYVLIDTAGMRKKGKVY
NCTC8325 IGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEYSYDGQDYVLIDTAGMRKKGKVY
Newman IGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEYSYDGQDYVLIDTAGMRKKGKVY
USA300_FPR3757 IGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEYSYDGQDYVLIDTAGMRKKGKVY
************************************************************
COL ESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDKRVAGYAHEQGKAVVIVVNKWDTVE
N315 ESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDKRVAGYAHEQGKAVVIVVNKWDTVE
NCTC8325 ESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDKRVAGYAHEQGKAVVIVVNKWDTVE
Newman ESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDKRVAGYAHEQGKAVVIVVNKWDTVE
USA300_FPR3757 ESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDKRVAGYAHEQGKAVVIVVNKWDTVE
************************************************************
COL KDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERTRLRTLFPYINEASENHKKRVQSSTLN
N315 KDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERTRLRTLFPYINEASENHKKRVQSSTLN
NCTC8325 KDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERTRLRTLFPYINEASENHKKRVQSSTLN
Newman KDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERTRLRTLFPYINEASENHKKRVQSSTLN
USA300_FPR3757 KDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERTRLRTLFPYINEASENHKKRVQSSTLN
************************************************************
COL EVVTDAISMNPTPTDKGRRLNVFYATQVAIEPPTFVVFVNDVELMHFSYKRYLENQIRAA
N315 EVVTDAISMNPTPTDKGRRLNVFYATQVAIEPPTFVVFVNDVELMHFSYKRYLENQIRAA
NCTC8325 EVVTDAISMNPTPTDKGRRLNVFYATQVAIEPPTFVVFVNDVELMHFSYKRYLENQIRAA
Newman EVVTDAISMNPTPTDKGRRLNVFYATQVAIEPPTFVVFVNDVELMHFSYKRYLENQIRAA
USA300_FPR3757 EVVTGAISMNPTPTDKGRRLNVFYATQVAIEPPTFVVFVNDVELMHFSYKRYLENQIRAA
****.*******************************************************
COL FGFEGTPIHIIARKRN
N315 FGFEGTPIHIIARKRN
NCTC8325 FGFEGTPIHIIARKRN
Newman FGFEGTPIHIIARKRN
USA300_FPR3757 FGFEGTPIHIIARKRN
****************
- ↑ Dominic Arpin, Armando Palacios, Kaustuv Basu, Joaquin Ortega
The binding of RbgA to a critical 50S assembly intermediate facilitates YphC function in bacterial ribosomal assembly.
Nucleic Acids Res: 2024;
[PubMed:39658043] [WorldCat.org] [DOI] (I a)Xiaoxiao Zhang, Kaige Yan, Yixiao Zhang, Ningning Li, Chengying Ma, Zhifei Li, Yanqing Zhang, Boya Feng, Jing Liu, Yadong Sun, Yanji Xu, Jianlin Lei, Ning Gao
Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly.
Nucleic Acids Res: 2014, 42(21);13430-9
[PubMed:25389271] [WorldCat.org] [DOI] (I p)