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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

Summary[edit | edit source]

  • pan ID?: SAUPAN004098000
  • symbol?:
  • synonym:
  • description?: hypothetical protein
  • strand?: -
  • coordinates?: 4378492..4378875
  • synteny block?: BlockID0031280
  • occurrence?: in 26% of 34 strains

virB3 : ICE6013 translocase accessory protein VirB3 [1]

Some staphylococci have acquired a Type IV secretion system encoded on an integrative conjugative element (ICE6013), which encodes a minimal conjugative transfer function that may have adapted to double as a small molecule translocation system. Translocase accessory proteins associate with translocase ATPases at the inner leaflet of the cell membrane and are essential for the formation of the translocon channel. Their exact role is unclear but may mediate protein-protein interactions, substrate recognition or proper ATPase orientation. Although these orthologues have not been structurally-confirmed, the entry to the translocon channel typically consists of a hexameric ring of VirB3-VirB4 heterodimers.

Orthologs[edit | edit source]

    COL:
    N315:
    NCTC8325:
    Newman:
    USA300_FPR3757:
    04-02981:
    08BA02176:
    11819-97:
    6850:
    71193:
    ECT-R 2:
    ED133:
    ED98:
    HO 5096 0412:
    SAEMRSA15_12310
    JH1:
    JH9:
    JKD6008:
    SAA6008_02637
    JKD6159:
    JSNZ:
    LGA251:
    M013:
    MRSA252:
    SAR1294
    MSHR1132:
    SAMSHR1132_15290
    MSSA476:
    Mu3:
    Mu50:
    MW2:
    RF122:
    ST398:
    T0131:
    SAT0131_02812
    TCH60:
    TW20:
    SATW20_27190
    USA300_TCH1516:
    USA300HOU_0632
    VC40:

Genome Viewer[edit | edit source]

COL
USA300_FPR3757

Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.307)


    COL             MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWFILFLLFWYLLQPVIMS
    USA300_FPR3757  MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWFILFLLFWYLLQPVIMS
                    ************************************************************

    COL             IGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVFNVQLRKVKLSYDEEVEYH
    USA300_FPR3757  IGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVFNVQLRKVKLSYDEEVEYH
                    ************************************************************

    COL             DKKITFK
    USA300_FPR3757  DKKITFK
                    *******

  1. Zita Nagy, Mónika Szabó, Michael Chandler, Ferenc Olasz
    Analysis of the N-terminal DNA binding domain of the IS30 transposase.
    Mol Microbiol: 2004, 54(2);478-88
    [PubMed:15469518] [WorldCat.org] [DOI] (P p)
    Davida S Smyth, D Ashley Robinson
    Integrative and sequence characteristics of a novel genetic element, ICE6013, in Staphylococcus aureus.
    J Bacteriol: 2009, 191(19);5964-75
    [PubMed:19648240] [WorldCat.org] [DOI] (I p)
    Minny Bhatty, Jenny A Laverde Gomez, Peter J Christie
    The expanding bacterial type IV secretion lexicon.
    Res Microbiol: 2013, 164(6);620-39
    [PubMed:23542405] [WorldCat.org] [DOI] (I p)
    Emily A Sansevere, Xiao Luo, Joo Youn Park, Sunghyun Yoon, Keun Seok Seo, D Ashley Robinson
    Transposase-Mediated Excision, Conjugative Transfer, and Diversity of ICE6013 Elements in Staphylococcus aureus.
    J Bacteriol: 2017, 199(8);
    [PubMed:28138100] [WorldCat.org] [DOI] (I e)