From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

Summary[edit | edit source]

  • pan ID?: SAUPAN004099000
  • symbol?:
  • synonym:
  • description?: hypothetical protein
  • strand?: -
  • coordinates?: 4378887..4379147
  • synteny block?: BlockID0031290
  • occurrence?: in 26% of 34 strains

virB2 : ICE6013 probable pilin subunit VirB2 [1]

Some staphylococci have acquired a Type IV secretion system encoded on an integrative conjugative element (ICE6013), which encodes a minimal conjugative transfer function that may have adapted to double as a small molecule translocation system. Pilin subunits are secreted through the VirB8 channel and self-assemble into a helical mating channel comprising the true extracellular component of the conjugative pilus. Typical VirB2 subunits are synthesized as prepilin subunits requiring proteolytic processing and macrocyclization before they are functional. This orthologue lacks the proteolytic processing signal, is the size of mature pilin and lacks a clear nearby VirB10-like pilin cyclase. However, it is structurally similar to pilin and located between virB8 and virB3 where virB2 prepilin coding sequences are typically found.

Orthologs[edit | edit source]

    COL:
    N315:
    NCTC8325:
    Newman:
    USA300_FPR3757:
    04-02981:
    08BA02176:
    11819-97:
    6850:
    71193:
    ECT-R 2:
    ED133:
    ED98:
    HO 5096 0412:
    SAEMRSA15_12300
    JH1:
    JH9:
    JKD6008:
    SAA6008_02638
    JKD6159:
    JSNZ:
    LGA251:
    M013:
    MRSA252:
    SAR1295
    MSHR1132:
    SAMSHR1132_15280
    MSSA476:
    Mu3:
    Mu50:
    MW2:
    RF122:
    ST398:
    T0131:
    SAT0131_02813
    TCH60:
    TW20:
    SATW20_27200
    USA300_TCH1516:
    USA300HOU_0633
    VC40:

Genome Viewer[edit | edit source]

COL
USA300_FPR3757

Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.307)


    COL             MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKWASGKYGHMVILFVVGG
    USA300_FPR3757  MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKWASGKYGHMVILFVVGG
                    ************************************************************

    COL             FLFLVSKGPEQVFNAIAGVWKMIFGG
    USA300_FPR3757  FLFLVSKGPEQVFNAIAGVWKMIFGG
                    **************************

  1. Erh-Min Lai, Ralf Eisenbrandt, Markus Kalkum, Erich Lanka, Clarence I Kado
    Biogenesis of T pili in Agrobacterium tumefaciens requires precise VirB2 propilin cleavage and cyclization.
    J Bacteriol: 2002, 184(1);327-30
    [PubMed:11741876] [WorldCat.org] [DOI] (P p)
    Davida S Smyth, D Ashley Robinson
    Integrative and sequence characteristics of a novel genetic element, ICE6013, in Staphylococcus aureus.
    J Bacteriol: 2009, 191(19);5964-75
    [PubMed:19648240] [WorldCat.org] [DOI] (I p)
    Minny Bhatty, Jenny A Laverde Gomez, Peter J Christie
    The expanding bacterial type IV secretion lexicon.
    Res Microbiol: 2013, 164(6);620-39
    [PubMed:23542405] [WorldCat.org] [DOI] (I p)
    Emily A Sansevere, Xiao Luo, Joo Youn Park, Sunghyun Yoon, Keun Seok Seo, D Ashley Robinson
    Transposase-Mediated Excision, Conjugative Transfer, and Diversity of ICE6013 Elements in Staphylococcus aureus.
    J Bacteriol: 2017, 199(8);
    [PubMed:28138100] [WorldCat.org] [DOI] (I e)
    Tamilarasi Shanmugasundarasamy, Deenadayalan Karaiyagowder Govindarajan, Kumaravel Kandaswamy
    A review on pilus assembly mechanisms in Gram-positive and Gram-negative bacteria.
    Cell Surf: 2022, 8;100077
    [PubMed:35493982] [WorldCat.org] [DOI] (I e)