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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004209000
- symbol?: —
- synonym:
- description?: peptidase U32
- peptidase U32
- U32 family peptidase
- peptidase, U32 family
- peptidase U32 family protein
- peptidase, U32 family small subunit (C1)
- peptidase family U32 family protein
- protease
- proteinase
- putative peptidase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4461816..4462739
- synteny block?: BlockID0032370
- occurrence?: in 100% of 33 strains
trhP2 (yrrN) : tRNA uridine(34) hydroxylation protein P2 [1]
Several transfer RNAs require modifications to provide maximal fidelity during protein synthesis. TrhP2 is a prephanate-dependent hydroxylation protein that, in combination with TrhP1, hydroxylates uridines at tRNA position 34 generating 5-hydroxyuridine ("ho5U") via a poorly-defined mechanism. Staphylococci may alternatively hydroxylate tRNA uridine(34) using an oxygen-dependent enzyme under aerobic conditions. TrhP1-TrhP2 is the first step of a two-step pathway resulting in sequential hydroxylation (TrhP1-TrhP2) and methoxylation (TrmR) steps targeting uridine(34). Staphylococcal tRNAs requiring this modification include: tRNAGUCC, tRNAVUAC, tRNAPUGG, tRNAAUGC, tRNAL3UAG, tRNATUGU, tRNASUGA.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1571
08BA02176:
C248_1655
11819-97:
MS7_1629
6850:
RSAU_001475
71193:
ST398NM01_1677
ECT-R 2:
ECTR2_1462
ED133:
SAOV_1611
ED98:
SAAV_1605
HO 5096 0412:
SAEMRSA15_15310
JH1:
SaurJH1_1704
JH9:
SaurJH9_1670
JKD6008:
SAA6008_01583
JKD6159:
SAA6159_01546
LGA251:
SARLGA251_15160
M013:
M013TW_1632
MRSA252:
SAR1692
MSHR1132:
SAMSHR1132_14500
MSSA476:
SAS1549
Mu3:
SAHV_1600
Mu50:
SAV1613
MW2:
MW1563
RF122:
SAB1484c
ST398:
SAPIG1677
T0131:
SAT0131_01710 (tuf)
TCH60:
HMPREF0772_11528
TW20:
SATW20_16080
USA300_TCH1516:
USA300HOU_1613
VC40:
SAVC_07315
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQMTEAVALAHKNDKKVY
N315 MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQMTEAVALAHKNDKKVY
NCTC8325 MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQMTEAVALAHKNDKKVY
Newman MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQMTEAVALAHKNDKKVY
USA300_FPR3757 MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQMTEAVALAHKNDKKVY
************************************************************
COL AAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVKAQENPIPLHWNAETLVTN
N315 AAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVKAQENPIPLHWNAETLVTN
NCTC8325 AAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVKAQENPIPLHWNAETLVTN
Newman AAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVKAQENPIPLHWNAETLVTN
USA300_FPR3757 AAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVKAQENPIPLHWNAETLVTN
************************************************************
COL HFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQVQGMTCMFQSKRMLLGNYYT
N315 HFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQVQGMTCMFQSKRMLLGNYYT
NCTC8325 HFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQVQGMTCMFQSKRMLLGNYYT
Newman HFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQVQGMTCMFQSKRMLLGNYYT
USA300_FPR3757 HFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQVQGMTCMFQSKRMLLGNYYT
************************************************************
COL FQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTHIMSPNDICLIEELAPFFEAGID
N315 FQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTHIMSPNDICLIEELAPFFEAGID
NCTC8325 FQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTHIMSPNDICLIEELAPFFEAGID
Newman FQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTHIMSPNDICLIEELAPFFEAGID
USA300_FPR3757 FQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTHIMSPNDICLIEELAPFFEAGID
************************************************************
COL SFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYEDEKFMLMDPIEEIQPDHRPFDEGF
N315 SFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYEDEKFMLIDPIEEIQPDHRPFDEGF
NCTC8325 SFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYEDEKFMLMDPIEEIQPDHRPFDEGF
Newman SFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYEDEKFMLMDPIEEIQPDHRPFDEGF
USA300_FPR3757 SFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYEDEKFMLMDPIEEIQPDHRPFDEGF
******************************************:*****************
COL LYKQTVY
N315 LYKQTVY
NCTC8325 LYKQTVY
Newman LYKQTVY
USA300_FPR3757 LYKQTVY
*******
- ↑ Yusuke Sakai, Satoshi Kimura, Tsutomu Suzuki
Dual pathways of tRNA hydroxylation ensure efficient translation by expanding decoding capability.
Nat Commun: 2019, 10(1);2858
[PubMed:31253794] [WorldCat.org] [DOI] (I e)Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)Charles T Lauhon
Identification and Characterization of Genes Required for 5-Hydroxyuridine Synthesis in Bacillus subtilis and Escherichia coli tRNA.
J Bacteriol: 2019, 201(20);
[PubMed:31358606] [WorldCat.org] [DOI] (I e)