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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004245000
- symbol?: tgt
- synonym:
- description?: queuine tRNA-ribosyltransferase
- queuine tRNA-ribosyltransferase
- tRNA guanosine(34) transglycosylase Tgt
- tRNA-guanine transglycosylase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4492741..4493880
- synteny block?: BlockID0032730
- occurrence?: in 100% of 33 strains
tgt : tRNA guanosine(34) transglycosylase [1]
Several transfer RNAs require modifications to provide maximal fidelity during protein synthesis. tRNA guanosine(34) transglycosylase exchanges guanosine with the heavily modified nucleobase 7-aminomethyl-7-deazaguanine (preQ1). PreQ1 is subsequently modified by QueA and QueG to generate the final nucleobase queuosine (Q). Staphylococcal tRNAs requiring this modification include: tRNAHGUG, tRNAYGUA, tRNANGUU, tRNADGUC.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1597 (tgt)
08BA02176:
C248_1682 (tgt)
11819-97:
MS7_1654 (tgt)
6850:
RSAU_001500 (tgt)
71193:
ST398NM01_1702
ECT-R 2:
ECTR2_1487 (tgt)
ED133:
SAOV_1636 (tgt)
ED98:
SAAV_1632 (tgt)
HO 5096 0412:
SAEMRSA15_15560 (tgt)
JH1:
SaurJH1_1729 (tgt)
JH9:
SaurJH9_1696 (tgt)
JKD6008:
SAA6008_01608 (tgt)
JKD6159:
SAA6159_01571 (tgt)
LGA251:
SARLGA251_15410 (tgt)
M013:
M013TW_1659
MRSA252:
SAR1719 (tgt)
MSHR1132:
SAMSHR1132_14750
MSSA476:
SAS1575 (tgt)
Mu3:
SAHV_1626 (tgt)
Mu50:
SAV1639 (tgt)
MW2:
MW1589 (tgt)
RF122:
SAB1508c (tgt)
ST398:
SAPIG1702 (tgt)
T0131:
SAT0131_01739
TCH60:
HMPREF0772_11503 (tgt)
TW20:
SATW20_16330 (tgt)
USA300_TCH1516:
USA300HOU_1638 (tgt)
VC40:
SAVC_07440 (tgt)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIIL
N315 MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIIL
NCTC8325 MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIIL
Newman MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIIL
USA300_FPR3757 MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIIL
************************************************************
COL GNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHT
N315 GNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHT
NCTC8325 GNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHT
Newman GNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHT
USA300_FPR3757 GNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHT
************************************************************
COL NGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQR
N315 NGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQR
NCTC8325 NGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQR
Newman NGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQR
USA300_FPR3757 NGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQR
************************************************************
COL PEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMP
N315 PEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMP
NCTC8325 PEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMP
Newman PEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMP
USA300_FPR3757 PEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMP
************************************************************
COL KDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLR
N315 KDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLR
NCTC8325 KDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLR
Newman KDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLR
USA300_FPR3757 KDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLR
************************************************************
COL PLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLD
N315 PLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLD
NCTC8325 PLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLD
Newman PLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLD
USA300_FPR3757 PLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLD
************************************************************
COL FKEEFFEQYGLNVENPKNF
N315 FKEEFFEQYGLNVENPKNF
NCTC8325 FKEEFFEQYGLNVENPKNF
Newman FKEEFFEQYGLNVENPKNF
USA300_FPR3757 FKEEFFEQYGLNVENPKNF
*******************
- ↑ Geoffrey Hutinet, Manal A Swarjo, Valérie de Crécy-Lagard
Deazaguanine derivatives, examples of crosstalk between RNA and DNA modification pathways.
RNA Biol: 2017, 14(9);1175-1184
[PubMed:27937735] [WorldCat.org] [DOI] (I p)Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)Jorge Díaz-Rullo, José Eduardo González-Pastor
tRNA queuosine modification is involved in biofilm formation and virulence in bacteria.
Nucleic Acids Res: 2023, 51(18);9821-9837
[PubMed:37638766] [WorldCat.org] [DOI] (I p)Valérie de Crécy-Lagard, Geoffrey Hutinet, José D D Cediel-Becerra, Yifeng Yuan, Rémi Zallot, Marc G Chevrette, R M Madhushi N Ratnayake, Marshall Jaroch, Samia Quaiyum, Steven Bruner
Biosynthesis and function of 7-deazaguanine derivatives in bacteria and phages.
Microbiol Mol Biol Rev: 2024, 88(1);e0019923
[PubMed:38421302] [WorldCat.org] [DOI] (I p)