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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004250000
- symbol?: obgE
- synonym:
- description?: GTPase ObgE
- GTPase ObgE
- Spo0B-related GTP-binding protein
- GTPase CgtA
- GTP-binding protein CgtA
- GTP-binding protein Obg
- GTP-binding protein Obg/CgtA
- obg family GTPase CgtA
- Spo0B-associated GTP-binding protein
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4497050..4498342
- synteny block?: BlockID0032780
- occurrence?: in 100% of 33 strains
obgE (cgtA): 70S ribosome anti-association factor and ribosome-associated GTPase ObgE [1]
Ribosome-associated GTPases including Era, RsgA, RbgA, HflX, ObgE, EngA and EngB are widely-conserved, stringent response alarmone-responsive ribosomal assembly cofactors
• ObgE displays transient 50S subunit binding and may act as a 70S anti-association factor checkpoint. Alarmone binding increases 50S affinity resulting in sequestration of large ribosome subunits and functional ribosome depletion.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1602 (obg)
08BA02176:
C248_1687 (obg)
11819-97:
MS7_1659 (cgtA)
6850:
RSAU_001505 (obgE)
71193:
ST398NM01_1707
ECT-R 2:
ECTR2_1492
ED133:
SAOV_1641
ED98:
SAAV_1637 (obgE)
HO 5096 0412:
SAEMRSA15_15610 (obg)
JH1:
SaurJH1_1734 (obgE)
JH9:
SaurJH9_1701 (obgE)
JKD6008:
SAA6008_01613 (obgE)
JKD6159:
SAA6159_01576 (obgE)
LGA251:
SARLGA251_15460 (obg)
M013:
M013TW_1664
MRSA252:
SAR1724 (obgE)
MSHR1132:
SAMSHR1132_14800
MSSA476:
SAS1580 (obgE)
Mu3:
SAHV_1631 (obgE)
Mu50:
SAV1644 (obgE)
MW2:
MW1594 (obgE)
RF122:
SAB1513c (obgE)
ST398:
SAPIG1707
T0131:
SAT0131_01744 (atoB)
TCH60:
HMPREF0772_11498 (cgtA)
TW20:
SATW20_16380 (obg)
USA300_TCH1516:
USA300HOU_1643 (obgE)
VC40:
SAVC_07465 (obgE)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASVVFEVDEGLRTLLDFRY
N315 MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASVVFEVDEGLRTLLDFRY
NCTC8325 MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASVVFEVDEGLRTLLDFRY
Newman MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASVVFEVDEGLRTLLDFRY
USA300_FPR3757 MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASVVFEVDEGLRTLLDFRY
************************************************************
COL QRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDEVLADLVEDGQRAVVAKGG
N315 QRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDEVLADLVEDGQRAVVAKGG
NCTC8325 QRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDEVLADLVEDGQRAVVAKGG
Newman QRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDEVLADLVEDGQRAVVAKGG
USA300_FPR3757 QRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDEVLADLVEDGQRAVVAKGG
************************************************************
COL RGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLADVGLVGFPSVGKSTLLSIVSK
N315 RGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLADVGLVGFPSVGKSTLLSIVSK
NCTC8325 RGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLADVGLVGFPSVGKSTLLSIVSK
Newman RGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLADVGLVGFPSVGKSTLLSIVSK
USA300_FPR3757 RGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLADVGLVGFPSVGKSTLLSIVSK
************************************************************
COL AKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIEGASDGVGLGHQFLRHVERTKVI
N315 AKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIEGASDGVGLGHQFLRHVERTKVI
NCTC8325 AKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIEGASDGVGLGHQFLRHVERTKVI
Newman AKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIEGASDGVGLGHQFLRHVERTKVI
USA300_FPR3757 AKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIEGASDGVGLGHQFLRHVERTKVI
************************************************************
COL VHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRPQIVVANKMDLPESQDNLNLFKEEI
N315 VHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRPQIVVANKMDLPESQDNLNLFKEEI
NCTC8325 VHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRPQIVVANKMDLPESQDNLNLFKEEI
Newman VHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRPQIVVANKMDLPESQDNLNLFKEEI
USA300_FPR3757 VHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRPQIVVANKMDLPESQDNLNLFKEEI
************************************************************
COL GEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDVDITVEEEESVGINRVLYKHTPSQDKF
N315 GEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDVDFTVEEEESVGINRVLYKHTPSQDKF
NCTC8325 GEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDVDFTVEEEESVGINRVLYKHTPSQDKF
Newman GEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDVDFTVEEEESVGINRVLYKHTPSQDKF
USA300_FPR3757 GEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDVDFTVEEEESVGINRVLYKHTPSQDKF
***********************************:************************
COL TISRDDDGAYVVSGNAIERMFKMTDFNSDPAVRRFARQMRSMGIDDALRERGCKNGDIVR
N315 TISRDDDGAYVVSGNAIERMFKMTDFNSDPAVRRFARQMRSMGIDDALRERGCKNGDIVR
NCTC8325 TISRDDDGAYVVSGNAIERMFKMTDFNSDPAVRRFARQMRSMGIDDALRERGCKNGDIVR
Newman TISRDDDGAYVVSGNAIERMFKMTDFNSDPAVRRFARQMRSMGIDDALRERGCKNGDIVR
USA300_FPR3757 TISRDDDGAYVVSGNAIERMFKMTDFNSDPAVRRFARQMRSMGIDDALRERGCKNGDIVR
************************************************************
COL ILGGEFEFVE
N315 ILGGEFEFVE
NCTC8325 ILGGEFEFVE
Newman ILGGEFEFVE
USA300_FPR3757 ILGGEFEFVE
**********
- ↑ Boya Feng, Chandra Sekhar Mandava, Qiang Guo, Jie Wang, Wei Cao, Ningning Li, Yixiao Zhang, Yanqing Zhang, Zhixin Wang, Jiawei Wu, Suparna Sanyal, Jianlin Lei, Ning Gao
Structural and functional insights into the mode of action of a universally conserved Obg GTPase.
PLoS Biol: 2014, 12(5);e1001866
[PubMed:24844575] [WorldCat.org] [DOI] (I e)Rebecca M Corrigan, Lauren E Bellows, Alison Wood, Angelika Gründling
ppGpp negatively impacts ribosome assembly affecting growth and antimicrobial tolerance in Gram-positive bacteria.
Proc Natl Acad Sci U S A: 2016, 113(12);E1710-9
[PubMed:26951678] [WorldCat.org] [DOI] (I p)