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⊟Summary[edit | edit source]
- pan ID?: SAUPAN005339000
- symbol?: mazF
- synonym:
- description?: mRNA interferase MazF
- mRNA interferase MazF
- PemK family protein
- PemK family DNA-binding protein
- toxin mazF, putative
- type II toxin-antitoxin system PemK/MazF family toxin
- Programmed cell death toxin YdcE
- Toxin MazF
- chpK
- endoribonuclease EndoA
- PemK family transcriptional regulator
- PemK-like protein
- transcriptional modulator of MazE/toxin MazF
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 5515021..5515383
- synteny block?: BlockID0041360
- occurrence?: in 100% of 33 strains
mazF: type II addiction module mRNA interferase toxin [1]
There is a crystal structure: 2MF2
Under normal conditions, MazE sequesters MazF rendering it inert. Under stress conditions, TrfA-augmented ClpCP proteolyzes MazE, MazF binds to UACAU sequences in mRNA transcripts and cleaves them resulting in de novo translation shutdown.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_2009 (ydcE)
08BA02176:
C248_2080
11819-97:
MS7_2085 (mazF)
6850:
RSAU_001908
71193:
ST398NM01_2109
ECT-R 2:
ECTR2_1924
ED133:
SAOV_2110c
ED98:
SAAV_2121
HO 5096 0412:
SAEMRSA15_19780
JH1:
SaurJH1_2143
JH9:
SaurJH9_2105
JKD6008:
SAA6008_02107 (mazF)
JKD6159:
SAA6159_01983 (mazF)
LGA251:
SARLGA251_18710
M013:
M013TW_2027
MRSA252:
SAR2156
MSHR1132:
SAMSHR1132_18930
MSSA476:
SAS1973
Mu3:
SAHV_2053
Mu50:
SAV2068
MW2:
MW1992
RF122:
SAB1953c
ST398:
SAPIG2109
T0131:
SAT0131_02225
TCH60:
HMPREF0772_11127
TW20:
SATW20_22080
USA300_TCH1516:
USA300HOU_2063 (pemK)
VC40:
SAVC_09225
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVE
N315 MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVE
NCTC8325 MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVE
Newman MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVE
USA300_FPR3757 MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVE
************************************************************
COL IEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN
N315 IEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN
NCTC8325 IEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN
Newman IEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN
USA300_FPR3757 IEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN
************************************************************
- ↑ Dongzhu Ma, Jonathan B Mandell, Niles P Donegan, Ambrose L Cheung, Wanyan Ma, Scott Rothenberger, Robert M Q Shanks, Anthony R Richardson, Kenneth L Urish
The Toxin-Antitoxin MazEF Drives Staphylococcus aureus Biofilm Formation, Antibiotic Tolerance, and Chronic Infection.
mBio: 2019, 10(6);
[PubMed:31772059] [WorldCat.org] [DOI] (I e)Christopher F Schuster, Ralph Bertram
Toxin-Antitoxin Systems of Staphylococcus aureus.
Toxins (Basel): 2016, 8(5);
[PubMed:27164142] [WorldCat.org] [DOI] (I e)Valentina Zorzini, Sarah Haesaerts, Ambrose Cheung, Remy Loris, Nico A J van Nuland
1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal MazF mRNA interferase.
Biomol NMR Assign: 2011, 5(2);157-60
[PubMed:21213075] [WorldCat.org] [DOI] (I p)Niles P Donegan, Earl T Thompson, Zhibiao Fu, Ambrose L Cheung
Proteolytic regulation of toxin-antitoxin systems by ClpPC in Staphylococcus aureus.
J Bacteriol: 2010, 192(5);1416-22
[PubMed:20038589] [WorldCat.org] [DOI] (I p)