PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0050 [new locus tag: SAUSA300_RS00265 ]
• pan locus tag^?: SAUPAN000803000
• symbol: SAUSA300_0050
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0050 [new locus tag: SAUSA300_RS00265 ]
• symbol: SAUSA300_0050
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 60849..61187
• length: 339
• essential: unknown

⊟Accession numbers

• Gene ID: 3913087 NCBI
• RefSeq: YP_492770 NCBI
• BioCyc: see SAUSA300_RS00265
• MicrobesOnline: 1291565 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
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  301
  
  ATGCATAAAAATGACAATAGTTACGTTTTATCAGCATTGAGTTATTTAAGTATCTTTTTC
  GCACCTGTCATTTTACCTTTATTTGTATGGATACTTGCTGATGAACCAACATCAGAACAT
  GGCAAAAAAGCATTCATTAATCATATTATGACTTGGGTTAGTCTTTTTATAGGTAGATTG
  GCATTTATTTTTTCTAAAGAAGTCTTTGATAAACCTTTGGATCATCAATTATTAATATTT
  AGCATTCTTTTAATTATTACTATTATATTCTTTTTAATTGCTTTAATATTATATATCTTG
  AACATTAACAGAGGAATTAAAATTCTATTAAAAAAATAA
  60
  120
  180
  240
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  339
  

⊟Protein

⊟General

• locus tag: SAUSA300_0050 [new locus tag: SAUSA300_RS00265 ]
• symbol: SAUSA300_0050
• description: hypothetical protein
• length: 112
• theoretical pI: 9.89698
• theoretical MW: 12975.6
• GRAVY: 0.926786

⊟Function

• TIGRFAM:
• TheSEED  :
  • FIG01109476: hypothetical protein
• PFAM:
  no clan defined DUF4870; Domain of unknown function (DUF4870) (PF09685; HMM-score: 22.3)
  and 4 more
  TMEM43; Transmembrane protein 43 (PF07787; HMM-score: 11)
  DUF2070; Predicted membrane protein (DUF2070) (PF09843; HMM-score: 10.5)
  PGG; Domain of unknown function (PF13962; HMM-score: 10.1)
  DUF3169; Protein of unknown function (DUF3169) (PF11368; HMM-score: 7.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 3
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.093889
  • TAT(Tat/SPI): 0.005176
  • LIPO(Sec/SPII): 0.005383
• predicted transmembrane helices (TMHMM): 3

⊟Accession numbers

• GI: 87160214 NCBI
• RefSeq: YP_492770 NCBI
• UniProt: A0A0H2XEJ2 UniProt

⊟Protein sequence

• MHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPTSEHGKKAFINHIMTWVSLFIGRLAFIFSKEVFDKPLDHQLLIFSILLIITIIFFLIALILYILNINRGIKILLKK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications