From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0127 [new locus tag: SAUSA300_RS00660 ]
  • pan locus tag?: SAUPAN000926000
  • symbol: SAUSA300_0127
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0127 [new locus tag: SAUSA300_RS00660 ]
  • symbol: SAUSA300_0127
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 145877..146284
  • length: 408
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAGCTCAATTATAGGAAAAATAGCAATTTGGATAGGCATCGTAGCTCAAATATATTTT
    AGTGTCGTTTTTGTTAGGATGATATCTATTAATATTGCTGGAGGATCTGATTACGAAACA
    ATTTTTTTATTAGGATTAATATTGGCTCTTTTCACTGTTTTACCAACCATCTTTACTGCG
    ATTTATATGGAAAGTTACTCTGTAATCGGAGGTGCACTTTTTATTGTTTATGCTATTATT
    GCACTGTGTTTATATAATTTCCTTTCGTCAATTTTATGGCTGATTGGTGGTATTTTGCTG
    ATTTGGAATAAATACTCAAAAGATGAATCGACAGACGAAAATGAAAAAGTTGATATTGAA
    AGTACAGAGAATCAATTTGAATCTAAAGATAAAATCACTAAAGAATAA
    60
    120
    180
    240
    300
    360
    408

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0127 [new locus tag: SAUSA300_RS00660 ]
  • symbol: SAUSA300_0127
  • description: hypothetical protein
  • length: 135
  • theoretical pI: 4.18191
  • theoretical MW: 15122.7
  • GRAVY: 0.757037

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01107877: hypothetical protein
  • PFAM:
    no clan defined Reticulon; Reticulon (PF02453; HMM-score: 16.5)
    DUF21; Domain of unknown function DUF21 (PF01595; HMM-score: 14.5)
    and 3 more
    Herpes_LMP1; Herpesvirus latent membrane protein 1 (LMP1) (PF05297; HMM-score: 8.5)
    Cupin (CL0029) Popeye; Popeye protein conserved region (PF04831; HMM-score: 8.2)
    no clan defined DUF973; Protein of unknown function (DUF973) (PF06157; HMM-score: 5.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00521
    • TAT(Tat/SPI): 0.000162
    • LIPO(Sec/SPII): 0.007829
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLLGLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIWNKYSKDESTDENEKVDIESTENQFESKDKITKE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_0127&oldid=94000"