PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0142 [new locus tag: SAUSA300_RS00745 ]
• pan locus tag^?: SAUPAN000954000
• symbol: phnE
• pan gene symbol^?: phnE1
• synonym:
• product: phosphonate ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0142 [new locus tag: SAUSA300_RS00745 ]
• symbol: phnE
• product: phosphonate ABC transporter permease
• replicon: chromosome
• strand: -
• coordinates: 161223..162038
• length: 816
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912815 NCBI
• RefSeq: YP_492859 NCBI
• BioCyc: see SAUSA300_RS00745
• MicrobesOnline: 1291657 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACACAGGAAATAGCAAAATATAATGTTCACACAAAAGCACACAAACGAAAATTGATT
  AAAAGATGGCTTATTGCAATTGTCGTCTTAGCTATTATCATCTGGGCATTTGCAGGTGTA
  CCAAGTTTAGAACTTAAAAGTAAATCATTAGAAATCTTAAAATCCATATTCAGCGGATTA
  TTCCATCCTGATATCAGCTATATCTATATACCAGATGGCGAAGACTTATTACGTGGTTTA
  CTTGAAACCTTTGCGATAGCCGTTGTAGGTACTTTCATCGCCGCAATTATCTGTATTCCA
  TTAGCATTTCTAGGTGCAAATAATATGGTAAAGCTACGCCCAGTTTCAGGTGTTAGCAAA
  TTTATTTTAAGTGTTATACGTGTCTTCCCAGAAATTGTAATGGCACTTATATTTATCAAA
  GCTGTTGGCCCAGGTTCATTTTCAGGTGTATTAGCTTTAGGTATCCATTCCGTAGGTATG
  CTTGGGAAACTTTTAGCTGAAGATATTGAAGGTCTAGATTTCAGTGCTGTAGAATCATTA
  AAGGCCAGTGGTGCGAATAAGATTAAAACACTCGTATTTGCAGTCATACCACAAATTATG
  CCTGCCTTTCTATCACTCATACTTTATCGCTTTGAACTAAACTTACGTTCAGCTTCTATA
  CTGGGGCTAATTGGGGCTGGTGGTATCGGGACACCACTCATATTTGCCATTCAAACACGT
  TCTTGGGACCGTGTAGGTATTATATTAATCGGTTTAGTACTAATGGTCGCAATTGTCGAT
  TTAATTTCCGGTTCAATCCGAAAACGTATTGTTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0142 [new locus tag: SAUSA300_RS00745 ]
• symbol: PhnE
• description: phosphonate ABC transporter permease
• length: 271
• theoretical pI: 10.4719
• theoretical MW: 29286.2
• GRAVY: 0.926568

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 267.8)
  and 6 more
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 24.7)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 21.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 19.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 14.5)
  Metabolism Transport and binding proteins Other energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 14.5)
  ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 9.9)
• TheSEED  :
  • ABC transporter, permease protein (cluster 12, methionine/phosphonates)
  Membrane Transport ABC transporters ABC transporter alkylphosphonate (TC 3.A.1.9.1)  Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1)
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 46.7)
  and 3 more
  no clan defined DUF4179; Domain of unknown function (DUF4179) (PF13786; HMM-score: 9.4)
  DUF5345; Family of unknown function (DUF5345) (PF17280; HMM-score: 9.2)
  SNARE; SNARE domain (PF05739; HMM-score: 8.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.236472
  • TAT(Tat/SPI): 0.00327
  • LIPO(Sec/SPII): 0.013786
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 87160389 NCBI
• RefSeq: YP_492859 NCBI
• UniProt: A0A0H2XEZ7 UniProt

⊟Protein sequence

• MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLELKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLAFLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGMLGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSASILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: phnE < phnE < phnC < SAUSA300_0145

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications