PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0151 [new locus tag: SAUSA300_RS00800 ]
• pan locus tag^?: SAUPAN000972000
• symbol: adhE
• pan gene symbol^?: adhE
• synonym:
• product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0151 [new locus tag: SAUSA300_RS00800 ]
• symbol: adhE
• product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 171207..173816
• length: 2610
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913550 NCBI
• RefSeq: YP_492866 NCBI
• BioCyc: see SAUSA300_RS00800
• MicrobesOnline: 1291666 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTTAACTATACCTGAAAAAGAAAATCGTGGATCGAAAGAACAAGAAGTGGCAATTATG
  ATTGATGCTCTAGCTGACAAAGGGAAAAAAGCATTAGAAGCATTATCTAAAAAGTCACAA
  GAAGAAATTGATCATATTGTTCATCAAATGAGCTTAGCAGCTGTTGATCAACATATGGTG
  CTAGCAAAATTAGCACATGAAGAAACTGGAAGAGGTATATACGAAGATAAAGCGATTAAA
  AATTTATACGCTTCTGAATATATATGGAATTCAATAAAAGACAATAAGACAGTAGGGATT
  ATTGGTGAAGATAAAGAAAAAGGATTAACGTATGTAGCGGAACCAATTGGTGTTATTTGT
  GGTGTTACGCCAACAACAAATCCTACGTCGACAACTATTTTTAAAGCGATGATTGCAATT
  AAGACAGGAAATCCAATCATTTTTGCATTCCATCCAAGTGCACAAGAATCGTCGAAGCGT
  GCAGCAGAAGTTGTATTAGAAGCGGCAATGAAGGCAGGTGCACCTAAAGATATTATTCAG
  TGGATTGAAGTGCCTTCTATCGAAGCAACAAAACAATTAATGAATCACAAAGGTATTGCA
  TTAGTTCTAGCAACAGGTGGTTCGGGCATGGTTAAGTCTGCATATTCAACTGGCAAACCG
  GCATTAGGTGTGGGACCAGGTAACGTGCCGTCTTACATTGAAAAAACAGCACACATTAAA
  CGTGCAGTAAATGATATCATTGGTTCAAAAACATTTGATAATGGTATGATTTGTGCTTCT
  GAACAAGTTGTAGTCATTGATAAAGAAATTTATAAAGATGTTACTAATGAATTTAAAGCA
  CATCAAGCATACTTTGTTAAAAAAGATGAATTACAACGCTTAGAAAATGCAATTATGAAT
  GAACAAAAAACAGGTATTAAGCCTGATATTGTCGGTAAATCTGCAGTTGAAATAGCTGAA
  TTAGCAGGTATACCTGTCCCCGAAAATACAAAACTTATCATAGCCGAAATTAGCGGTGTA
  GGTTCAGACTATCCGTTATCTCGTGAAAAATTATCTCCAGTATTAGCCTTAGTAAAAGCC
  CAATCTACAAAACAAGCATTTCAAATTTGTGAAGACACACTACATTTTGGTGGATTAGGA
  CACACAGCCGTTATCCATACAGAAGATGAAACATTACAAAAAGATTTTGGACTAAGAATG
  AAAGCTTGTCGTGTACTTGTAAATACACCATCAGCGGTTGGAGGTATTGGTGATATGTAT
  AACGAATTGATTCCGTCTTTAACATTAGGTTGTGGTTCCTACGGTAGAAACTCAATTTCA
  CATAATGTTAGTGCGACAGATTTATTAAACATTAAAACGATTGCTAAACGACGTAATAAT
  ACTCAAATTTTCAAGGTGCCTGCTCAAATTTATTTTGAAGAAAATGCAATCATGAGTCTA
  ACAACAATGGACAAGATTGAAAAAGTGATGATTGTCTGTGACCCTGGTATGGTAGAATTC
  GGTTATACAAAAACAGTTGAGAATGTATTAAGACAAAAAACGGAACAGCCTCAAATTAAA
  ATATTTAGCGAAGTCGAACCGAACCCATCAACTAATACAGTATATAAAGGTCTGGAAATG
  ATGGTTGATTTCCAACCGGATACAATCATTGCACTTGGTGGTGGTTCAGCGATGGATGCT
  GCAAAAGCAATGTGGATGTTCTTTGAACACCCTGAGACATCATTCTTCGGTGCTAAACAA
  AAGTTCCTAGACATCGGTAAACGTACTTATAAAATAGGCATGCCTGAAAATGCGACGTTC
  ATTTGTATCCCTACGACATCAGGTACAGGTTCAGAAGTAACACCATTTGCAGTTATCACA
  GATAGTGAAACAAATGTAAAATATCCGTTGGCTGATTTTGCTTTAACACCTGACGTTGCA
  ATTATTGACCCTCAATTTGTGATGAGTGTGCCAAAAAGCGTTACAGCAGATACAGGAATG
  GATGTACTAACGCATGCAATGGAATCATATGTATCTGTAATGGCTTCAGACTACACAAGA
  GGTTTGAGTCTACAAGCGATTAAATTGACGTTCGAATATTTAAAATCATCTGTTGAAAAG
  GGTGATAAAGTTTCAAGAGAGAAAATGCATAACGCATCAACTTTGGCTGGTATGGCATTT
  GCAAATGCATTCTTAGGCATTGCACACTCAATTGCGCATAAAATTGGTGGCGAATATGGT
  ATTCCGCATGGTAGAGCGAATGCGATATTACTACCGCATATTATCCGTTATAATGCCAAA
  GACCCGCAAAAACATGCATTATTCCCTAAATATGAGTTCTTCAGAGCAGATACAGATTAT
  GCAGATATTGCCAAATTCTTAGGATTAAAAGGTAATACGACAGAAGCACTCGTAGAATCA
  TTAGCTAAAGCTGTCTACGAATTAGGTCAATCAGTCGGAATTGAAATGAATTTGAAATCA
  CAAGGTGTGTCTGAAGAAGAATTAAATGAGTCAATTGATAGAATGGCAGAGCTCGCATTT
  GAAGATCAATGTACAACTGCTAATCCTAAAGAAGCACTAATCAGTGAAATCAAAGATATC
  ATTCAAACATCATATGATTATAAGCAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0151 [new locus tag: SAUSA300_RS00800 ]
• symbol: AdhE
• description: bifunctional acetaldehyde-CoA/alcohol dehydrogenase
• length: 869
• theoretical pI: 5.84639
• theoretical MW: 94944.4
• GRAVY: -0.149252

⊟Function

• reaction:
  EC 1.1.1.1?  ExPASy
  Alcohol dehydrogenase A primary alcohol + NAD⁺ = an aldehyde + NADH A secondary alcohol + NAD⁺ = a ketone + NADH
• TIGRFAM:
  acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 514.9)
  and 13 more
  Metabolism Energy metabolism Sugars lactaldehyde reductase (TIGR02638; EC 1.1.1.77; HMM-score: 280)
  phosphonate metabolism-associated iron-containing alcohol dehydrogenase (TIGR03405; HMM-score: 192.2)
  NDMA-dependent methanol dehydrogenase (TIGR04266; EC 1.1.99.37; HMM-score: 109.3)
  Metabolism Amino acid biosynthesis Glutamate family glutamate-5-semialdehyde dehydrogenase (TIGR00407; EC 1.2.1.41; HMM-score: 55.2)
  Cellular processes Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 55.2)
  Metabolism Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 54.5)
  5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 39.6)
  Metabolism Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 37.5)
  Metabolism Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 33.8)
  putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 25.3)
  Metabolism Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 24.6)
  Metabolism Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 24.1)
  Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 14.9)
• TheSEED  :
  • Acetaldehyde dehydrogenase (EC 1.2.1.10)
  • Alcohol dehydrogenase (EC 1.1.1.1)
  Carbohydrates Central carbohydrate metabolism Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate  Acetaldehyde dehydrogenase (EC 1.2.1.10)
  and 5 more
  Carbohydrates Fermentation Fermentations: Lactate  Acetaldehyde dehydrogenase (EC 1.2.1.10)
  Carbohydrates Fermentation Fermentations: Lactate  Alcohol dehydrogenase (EC 1.1.1.1)
  Carbohydrates Fermentation Fermentations: Mixed acid  Acetaldehyde dehydrogenase (EC 1.2.1.10)
  Carbohydrates Fermentation Fermentations: Mixed acid  Alcohol dehydrogenase (EC 1.1.1.1)
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Alcohol dehydrogenase (EC 1.1.1.1)
• PFAM:
  DHQS (CL0224) Fe-ADH; Iron-containing alcohol dehydrogenase (PF00465; HMM-score: 377.4)
  and 3 more
  ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 94.5)
  DHQS (CL0224) Fe-ADH_2; Iron-containing alcohol dehydrogenase (PF13685; HMM-score: 53.6)
  HTH (CL0123) HTH_40; Helix-turn-helix domain (PF14493; HMM-score: 7.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002759
  • TAT(Tat/SPI): 0.000416
  • LIPO(Sec/SPII): 0.000451
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160829 NCBI
• RefSeq: YP_492866 NCBI
• UniProt: A0A0H2XG10 UniProt

⊟Protein sequence

• MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIDHIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIGEDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKRAAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTGKPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTNEFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLIIAEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETLQKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLNIKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVENVLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWMFFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSETNVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGLSLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYGIPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALVESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISEIKDIIQTSYDYKQ

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: Rex (repression) regulon

  Rex

  (TF)

  important in Energy metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications