From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0189 [new locus tag: SAUSA300_RS00990 ]
  • pan locus tag?: SAUPAN001029000
  • symbol: entB
  • pan gene symbol?: —
  • synonym:
  • product: isochorismatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0189 [new locus tag: SAUSA300_RS00990 ]
  • symbol: entB
  • product: isochorismatase
  • replicon: chromosome
  • strand: -
  • coordinates: 218795..219292
  • length: 498
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGATTATGCCCCATATACAGCTGAAAATGTCGTTCAAAACGCTAATAAATTAATAGAT
    GTTTTTAGAAAAAACAATGGCTTTATCGCTTTTGTTCGCGTGAATTTCTATGATGGTAAA
    GATGTATTGCAACCAAATGCAATGATCTCATTACCACCAAAAGAAGGCGACGACTACAGT
    CGTTTCCATCATTTATTAGACAAGAGAGATGACGATTTTGTCATAGACAAACGACATTTT
    AGTGCATTTGTAGGAACAGATTTGGACTTACAATTGCGACGTCGAGGAATTGATACGATT
    GTTCTTGGTGGTGTCGCAACGCATATTGGCGTAGATACGACAGCGCGAGATGCCTATCAA
    TTAAACTACAATCAGTTTTTTGTTACAGATATGATGAGTGCACAAAACGAAACGCTACAT
    CAATTTCCAATAGATAATGTATTCCCATTGATGGGACAAACAATAACTACAAACGACTTT
    CTAAATATATTGAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    498

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0189 [new locus tag: SAUSA300_RS00990 ]
  • symbol: EntB
  • description: isochorismatase
  • length: 165
  • theoretical pI: 4.91313
  • theoretical MW: 18895.1
  • GRAVY: -0.297576

⊟Function[edit | edit source]

  • reaction:
    EC 3.3.2.1?  ExPASy
    Isochorismatase Isochorismate + H2O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
  • TIGRFAM:
    pyrimidine utilization protein B (TIGR03614; HMM-score: 54.4)
  • TheSEED  :
    • Nicotinamidase/isochorismatase family protein
    Cofactors, Vitamins, Prosthetic Groups, Pigments NAD and NADP NAD and NADP cofactor biosynthesis global  Nicotinamidase/isochorismatase family protein
  • PFAM:
    no clan defined Isochorismatase; Isochorismatase family (PF00857; HMM-score: 116.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002446
    • TAT(Tat/SPI): 0.000157
    • LIPO(Sec/SPII): 0.000435
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDYAPYTAENVVQNANKLIDVFRKNNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSAFVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLHQFPIDNVFPLMGQTITTNDFLNILN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_0189&oldid=99261"