PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0227 [new locus tag: SAUSA300_RS01205 ]
• pan locus tag^?: SAUPAN001094000
• symbol: fadD
• pan gene symbol^?: fadD
• synonym:
• product: acyl-CoA dehydrogenase FadD

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0227 [new locus tag: SAUSA300_RS01205 ]
• symbol: fadD
• product: acyl-CoA dehydrogenase FadD
• replicon: chromosome
• strand: -
• coordinates: 272496..273707
• length: 1212
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913734 NCBI
• RefSeq: YP_492941 NCBI
• BioCyc: see SAUSA300_RS01205
• MicrobesOnline: 1291742 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1201
  
  ATGACATTTGAAAAAGAAACGGTCTTAAAAACATTATTTCCTGAAGATGTACTTAGTATT
  GCTAAAGGTTTAACAGACGGTGAAGTCGAATTTTTACAACAAGTAGATTCATTGCTAGAA
  AGTAAGTACCGTGAAAATATTAATCAACATTGGATAGACGCTACTGTACCCGAGGACTAT
  TTTAAAGATCTGGGAGAATTAAATTATTTTAACAATCCATTACTTTACAAGGATCGTCCA
  AACGCCAAAATGCCTAGTCAACTATTTCAGTTTTTCATGTCTTACCTACTCGCGCGATTT
  GATATTTCCTTAGCTACCCTACTCGGTGTTCACCAAGGTTTAGGGCATAACACTTTCTAT
  TTCGGAGGTAGCAAAGAACAAATTGCGAAATATGTACCTAAATTACAGTCACATGAACTG
  CGTACATGCTTTGCTTTAACTGAACCAGAACACGGTTCGGACGTTGCGGGAGGTCTTGAA
  ACAGTCGCTGAACGCCAAGGCGATACTTGGGTTATCAATGGTGAAAAGAAATGGATTGGT
  GGTGCACATGTATCTGATGTCATTCCAGTATTCGCAGTAAATAAAGAAACTGGCAAACCC
  CATTGCTTTGTAGTCAGACCAGAACAAGATGGCGTCGATATTGAAGTCATTGATAATAAA
  ATCGCACTTCGCATTGTTCCTAACGCCCTAATTAAATTAACTAATGTCAAAGTAGATGAA
  GCGGATCGCTTACAAAACATAACAAGCTTTAAAGATATTGCCAAAATTCTTTATTCAACG
  AGAGCAGGCGTTGCTTATATGGCTACAGGTGGTATGGCTGGCGCTTTACGTGCCACATTA
  GATTATGTCACTGAGCGTAAGCAATTCGGCAAACCAATTAGTAAATATCAGTTAATACAA
  GAAAAGCTAGCAATGATGCAAGGTAATTTAGCTCAAGCAATGGCAACATGTGCTCAATTA
  GCTAATATGCAAGCACATGGTGAATATGACGAGGTTGCAACTTCAACGGCGAAGATGATG
  AATGCCTTACGTTTGCGTGAGACAGTAGCTATGGGCCGCGGTATTACAGGTGGTAATGGC
  ATACTAGCTGACGATTATGATATTGCACGTTTCTTCTCTGATGCAGAAGCGATTTACACG
  TACGAAGGTACACATGAAATTAATGCCTTAGTAATTGGACGCGCTTTGACTGGAGATTCT
  GCTTTCGTATAA
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  1212
  

⊟Protein

⊟General

• locus tag: SAUSA300_0227 [new locus tag: SAUSA300_RS01205 ]
• symbol: FadD
• description: acyl-CoA dehydrogenase FadD
• length: 403
• theoretical pI: 5.17502
• theoretical MW: 44728.7
• GRAVY: -0.168983

⊟Function

• reaction:
  EC 1.3.8.6?  ExPASy
  Glutaryl-CoA dehydrogenase (ETF) Glutaryl-CoA + electron-transfer flavoprotein = crotonyl-CoA + CO₂ + reduced electron-transfer flavoprotein
  EC 1.3.99.7?  ExPASy
  Transferred entry: 1.3.8.6
• TIGRFAM:
  cyclohexanecarboxyl-CoA dehydrogenase (TIGR03207; EC 1.1.1.-; HMM-score: 73)
  pimeloyl-CoA dehydrogenase, small subunit (TIGR03203; EC 1.3.1.62; HMM-score: 59.4)
  and 2 more
  pimeloyl-CoA dehydrogenase, large subunit (TIGR03204; EC 1.3.1.62; HMM-score: 55.5)
  Metabolism Central intermediary metabolism Sulfur metabolism sulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 35)
• TheSEED  :
  • Acyl-CoA dehydrogenase, long-chain specific (EC 1.3.8.8)
• PFAM:
  Acyl-CoA_dh (CL0087) Acyl-CoA_dh_1; Acyl-CoA dehydrogenase, C-terminal domain (PF00441; HMM-score: 93.9)
  and 4 more
  no clan defined Acyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 69.6)
  Acyl-CoA_dh (CL0087) Acyl-CoA_dh_2; Acyl-CoA dehydrogenase, C-terminal domain (PF08028; HMM-score: 31.4)
  AcylCoA_ox_dh_N (CL0544) Acyl-CoA_dh_N; Acyl-CoA dehydrogenase, N-terminal domain (PF02771; HMM-score: 30.6)
  no clan defined DUF3726; Protein of unknown function (DUF3726) (PF12525; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: FAD
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003255
  • TAT(Tat/SPI): 0.000279
  • LIPO(Sec/SPII): 0.000562
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161145 NCBI
• RefSeq: YP_492941 NCBI
• UniProt: A0A0H2XHZ4 UniProt

⊟Protein sequence

• MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYRENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDISLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLETVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVIDNKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGALRATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVATSTAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVIGRALTGDSAFV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: fadD < fadE < SAUSA300_0229

⊟Regulation

• regulator: CcpA regulon

  CcpA

  (TF)

  important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications