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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0234 [new locus tag: SAUSA300_RS01240 ]
  • pan locus tag?: SAUPAN001105000
  • symbol: SAUSA300_0234
  • pan gene symbol?: hmp
  • synonym:
  • product: putative flavohemoprotein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0234 [new locus tag: SAUSA300_RS01240 ]
  • symbol: SAUSA300_0234
  • product: putative flavohemoprotein
  • replicon: chromosome
  • strand: -
  • coordinates: 281209..282354
  • length: 1146
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCTTACAGAACAAGAGAAAGACATTATCAAACAAACGGTGCCTTTACTTAAAGAGAAA
    GGGACAGAAATTACGTCAATCTTTTATCCAAAAATGTTTAAAGCGCATCCTGAACTTTTA
    AACATGTTTAATCAAACGAACCAAAAACGAGGCATGCAATCTTCAGCATTAGCACAAGCT
    GTAATGGCCGCAGCGGTTAATATCGATAACTTAAGTGTTATTAAACCAGTCATTATGCCA
    GTCGCATATAAACACTGCGCACTACAAGTTTATGCTGAACATTATCCAATTGTGGGGAAA
    AATTTATTAAAAGCCATTCAAGACGTGACAGGATTAGAAGAAAATGACCCTGTCATTCAA
    GCTTGGGCAAAAGCATATGGCGTTATTGCGGATGTGTTCATCCAAATTGAAAAAGAAATT
    TATGATCAAATGATGTGGATTGGTTTTAAACCATTCAAAATTACCAATATTAAACAAGAA
    TCTGAAGACATTAAATCATTTACAGTTGAAACTGAAGAATATGACTTTAGTGAATTTACA
    CCAGGCCAATACATCACAGTTGATGTTTCTAGTGATAAACTTCCATATAGAGCTAAACGT
    CACTATTCTATCGTATCAGGTGAAAAAAACCATTTAACTTTTGGCGTTAAACGTGATGTC
    ACAACAGAACATGAAGGCGAAGTTTCAACAATTTTACACGATGAGATTAAAGAAGGCGAT
    ATGATTAATTTAGCTGCGCCTGTAGGTGGATTCGTATTAGAGAATACGACTGAACCACAA
    CTTTTCTTAGGTTCAGGTATTGGTGTTACACCTTTAGTAGCTATGTATGAAGCTGCCTCT
    GCCAAAGGTTTAGATACACAGATGGTTCAAGTTGCTGAAAATGAACAACATTTACCTTTC
    AAAGACAACTTCAACAGTATCGCAAGCCATCATGACAACGCTAAATTATATACACACTTA
    AAAGATAAACAAGGCTATATTGGTGCTGAAGAATTACAAGTATTTTTAGCAAATAAACCT
    GAAATTTATATCTGTGGTGGTACAAAATTCTTACAATCTATGATCGAAGCACTTAAATCT
    TTAAATTACGATATGGATCGCGTACACTACGAAACATTTATTCCAAGACTAAGCGTTGCA
    GTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1146

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0234 [new locus tag: SAUSA300_RS01240 ]
  • symbol: SAUSA300_0234
  • description: putative flavohemoprotein
  • length: 381
  • theoretical pI: 5.16615
  • theoretical MW: 42906.8
  • GRAVY: -0.212861

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Other phenylacetate-CoA oxygenase/reductase, PaaK subunit (TIGR02160; HMM-score: 56.5)
    and 1 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit (TIGR01941; EC 1.6.5.-; HMM-score: 26.5)
  • TheSEED  :
    • Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)
    Stress Response Oxidative stress Glutaredoxins  Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)
    and 2 more
    Stress Response Stress Response - no subcategory Bacterial hemoglobins  Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)
    Stress Response Stress Response - no subcategory Flavohaemoglobin  Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)
  • PFAM:
    FAD_Lum_binding (CL0076) FAD_binding_6; Oxidoreductase FAD-binding domain (PF00970; HMM-score: 37.3)
    Globin (CL0090) Globin; Globin (PF00042; HMM-score: 32.1)
    and 1 more
    NAD_Ferredoxin (CL0091) NAD_binding_1; Oxidoreductase NAD-binding domain (PF00175; HMM-score: 22.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00738
    • TAT(Tat/SPI): 0.001641
    • LIPO(Sec/SPII): 0.000377
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFNQTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNLLKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQESEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKRDVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMYEAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQVFLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • data available for N315

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]