PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0322 [new locus tag: SAUSA300_RS01715 ]
• pan locus tag^?: SAUPAN001852000
• symbol: SAUSA300_0322
• pan gene symbol^?: —
• synonym:
• product: Oye family NADH-dependent flavin oxidoreductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0322 [new locus tag: SAUSA300_RS01715 ]
• symbol: SAUSA300_0322
• product: Oye family NADH-dependent flavin oxidoreductase
• replicon: chromosome
• strand: -
• coordinates: 375629..376828
• length: 1200
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913165 NCBI
• RefSeq: YP_493036 NCBI
• BioCyc: see SAUSA300_RS01715
• MicrobesOnline: 1291837 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTACCGATACAAACCACTTTTACAATCAATCCACTTACCTAATGGCATCAAAATTTCG
  AATCGCTTTGTATTATCACCAATGACCGTAAACACATCAACAAAAGAAGGCTATATTACA
  AAAGAAGACTTGGCTTATGCTGCACGTCGCTCTAATTCAGCTGGAATGCAAGTCACCGGC
  GCTGCTTACATTGAACCTTATGGTCAATTGTTTGAATATGGCTTTAATATTGATCATGAT
  GCTTGTATCCCTGGATTAACTAACATGGCATCTACGATGAAACAACATGGTAGCCTCGCT
  ATTATCCAGCTAGCGCATGCTGGTCGTTTTTCAAATCAAGCCATTTTAAATTTTGGAAAG
  GTGTATGGACCAAGCCCTATGACATTGCATTCACCGATTGAACACGATGTTATCGCCATG
  TCTCATGAGAAAATCAATAGCATCATTCAGCAGTATCGCGATGCTACATTACGCGCGATT
  AAAGCAGGTTTTGATGGTGTGGAAATTTCGATTGCACAACGTTTGCTGATTCAAACATTT
  TTCTCAACTTTTTCGAATAAACGTACAGACCATTACGGTGCCGACTCATTAAAAAATAGT
  GCAAGATTATGTTTAGAAGTTATGCGTGCGGTACAAGAAGTGATTGATAAAGAAGCACCT
  GACAATTTTATTTTAGGATTTCGAGCAACGCCAGAAGAAACCAGAGGCAGTGATTTAGGA
  TATACCATTGACGAGTTCAACCAATTAATCAATTGGGTCATGGACGTTTCAGACATCCAA
  TACTTAGCAATCGCTAGCTGGGGTCGACATATTTATCAAAATACGTCTCGTACACCAGGT
  GATCATTTCGGTCGACCAGTTAACCAAATTGTCTATGAACATTTAGCAGGTCGTATCCCT
  TTAATTGCAAGTGGCGGGATTAATTCTCCAGAGTCTGCTTTAGATGCTTTACAACATGCA
  GATATGGTCGGCATGTCTTCGCCTTTTGTCACAGAACCTGACTTTGTGCATAAACTTGCT
  GAACAGCGACCACATGATATCAATCTTGAATTTTCTATGGCAGATTTAGAAGATTTAGCC
  ATTCCACATGCTGCTTTTAAAGATATTGTTAAAATGATGGATTATGGGGAAGGTCTAAAG
  AAGCATACCCGTGACGCTTTACGTCAATTGGAACAGAATTATCATGACTCATCGTCTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0322 [new locus tag: SAUSA300_RS01715 ]
• symbol: SAUSA300_0322
• description: Oye family NADH-dependent flavin oxidoreductase
• length: 399
• theoretical pI: 6.36372
• theoretical MW: 44623.2
• GRAVY: -0.293233

⊟Function

• reaction:
  EC 1.-.-.-?  ExPASy
• TIGRFAM:
  mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 143.3)
  and 3 more
  mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 100.7)
  3-isopropylmalate dehydratase, small subunit (TIGR02087; HMM-score: 11.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 11.6)
• TheSEED  :
  • Probable NADH-dependent flavin oxidoreductase in cluster with COG2110
• PFAM:
  TIM_barrel (CL0036) Oxidored_FMN; NADH:flavin oxidoreductase / NADH oxidase family (PF00724; HMM-score: 183.3)
  and 4 more
  DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 16.9)
  His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 16.2)
  DHDPS; Dihydrodipicolinate synthetase family (PF00701; HMM-score: 12.8)
  Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.057426
  • TAT(Tat/SPI): 0.002729
  • LIPO(Sec/SPII): 0.004003
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161663 NCBI
• RefSeq: YP_493036 NCBI
• UniProt: A0A0H2XJ96 UniProt

⊟Protein sequence

• MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDLAYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAIKAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNSARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSDIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0321 < SAUSA300_0322

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications